OCA at 17:35, 29 November 2023

OCA — Wed, 29 Nov 2023 17:35:03 GMT

←Older revision		Revision as of 17:35, 29 November 2023
Line 1:		Line 1:

	==Crystal structure of human Suv3 monomer==		==Crystal structure of human Suv3 monomer==
-	<StructureSection load='7w1r' size='340' side='right'caption='[[7w1r]]' scene=''>	+	<StructureSection load='7w1r' size='340' side='right'caption='[[7w1r]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W1R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7w1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W1R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w1r OCA], [https://pdbe.org/7w1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w1r RCSB], [https://www.ebi.ac.uk/pdbsum/7w1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w1r ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w1r OCA], [https://pdbe.org/7w1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w1r RCSB], [https://www.ebi.ac.uk/pdbsum/7w1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w1r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SUV3_HUMAN SUV3_HUMAN] Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.<ref>PMID:12466530</ref> <ref>PMID:15096047</ref> <ref>PMID:17352692</ref> <ref>PMID:17961633</ref> <ref>PMID:18678873</ref> <ref>PMID:19509288</ref> <ref>PMID:19864255</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human Suv3 is a unique homodimeric helicase that constitutes the major component of the mitochondrial degradosome to work cooperatively with exoribonuclease PNPase for efficient RNA decay. However, the molecular mechanism of how Suv3 is assembled into a homodimer to unwind RNA remains elusive. Here, we show that dimeric Suv3 preferentially binds to and unwinds DNA-DNA, DNA-RNA, and RNA-RNA duplexes with a long 3' overhang (>/=10 nucleotides). The C-terminal tail (CTT)-truncated Suv3 (Suv3DeltaC) becomes a monomeric protein that binds to and unwinds duplex substrates with ~six to sevenfold lower activities relative to dimeric Suv3. Only dimeric Suv3, but not monomeric Suv3DeltaC, binds RNA independently of ATP or ADP, and is capable of interacting with PNPase, indicating that dimeric Suv3 assembly ensures its continuous association with RNA and PNPase during ATP hydrolysis cycles for efficient RNA degradation. We further determined the crystal structure of the apo-form of Suv3DeltaC, and SAXS structures of dimeric Suv3 and PNPase-Suv3 complex, showing that dimeric Suv3 caps on the top of PNPase via interactions with S1 domains, and forms a dumbbell-shaped degradosome complex with PNPase. Overall, this study reveals that Suv3 is assembled into a dimeric helicase by its CTT for efficient and persistent RNA binding and unwinding to facilitate interactions with PNPase, promote RNA degradation, and maintain mitochondrial genome integrity and homeostasis.
		+
		+	Dimeric assembly of human Suv3 helicase promotes its RNA unwinding function in mitochondrial RNA degradosome for RNA decay.,Jain M, Golzarroshan B, Lin CL, Agrawal S, Tang WH, Wu CJ, Yuan HS Protein Sci. 2022 May;31(5):e4312. doi: 10.1002/pro.4312. PMID:35481630<ref>PMID:35481630</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w1r" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jain M]]		[[Category: Jain M]]
	[[Category: Yuan HS]]		[[Category: Yuan HS]]

OCA at 06:21, 12 May 2022

OCA — Thu, 12 May 2022 06:21:35 GMT

←Older revision		Revision as of 06:21, 12 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w1r ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of human Suv3 monomer==
-		+	<StructureSection load='7w1r' size='340' side='right'caption='[[7w1r]]' scene=''>
-	~~Authors~~: ~~Jain~~, M., ~~Yuan~~, H.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W1R FirstGlance]. <br>
-	~~Description: Crystal structure of human Suv3 monomer~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w1r OCA], [https://pdbe.org/7w1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w1r RCSB], [https://www.ebi.ac.uk/pdbsum/7w1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w1r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yuan, H.S~~]]	+	__TOC__
-	[[Category: ~~Jain, M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jain M]]
		+	[[Category: Yuan HS]]

OCA at 14:13, 22 December 2021

OCA — Wed, 22 Dec 2021 14:13:18 GMT

←Older revision		Revision as of 14:13, 22 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w1r is ON HOLD	+	The entry 7w1r is ON HOLD until Paper Publication

	Authors: Jain, M., Yuan, H.S.		Authors: Jain, M., Yuan, H.S.

OCA: Protected "7w1r" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:18:43 GMT

Protected "7w1r" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w1r is ON HOLD Authors: Jain, M., Yuan, H.S. Description: Crystal structure of human Suv3 monomer Category: Unreleased Structures [[Category: ...

OCA — Wed, 01 Dec 2021 05:18:42 GMT

New page: '''Unreleased structure''' The entry 7w1r is ON HOLD Authors: Jain, M., Yuan, H.S. Description: Crystal structure of human Suv3 monomer Category: Unreleased Structures [[Category: ...

New page

'''Unreleased structure'''

The entry 7w1r is ON HOLD

Authors: Jain, M., Yuan, H.S.

Description: Crystal structure of human Suv3 monomer
[[Category: Unreleased Structures]]
[[Category: Yuan, H.S]]
[[Category: Jain, M]]

7w1r - Revision history

OCA at 17:35, 29 November 2023

OCA at 06:21, 12 May 2022

OCA at 14:13, 22 December 2021

OCA: Protected "7w1r" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7w1r is ON HOLD Authors: Jain, M., Yuan, H.S. Description: Crystal structure of human Suv3 monomer Category: Unreleased Structures [[Category: ...