7w6z - Revision history

OCA at 18:44, 7 September 2022

OCA — Wed, 07 Sep 2022 18:44:04 GMT

←Older revision		Revision as of 18:44, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/C7R5Z1_KANKD C7R5Z1_KANKD]]		[[https://www.uniprot.org/uniprot/C7R5Z1_KANKD C7R5Z1_KANKD]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Site-2 proteases are a conserved family of intramembrane proteases that cleave transmembrane substrates to regulate signal transduction and maintain proteostasis. Here, we elucidated crystal structures of inhibitor-bound forms of bacterial site-2 proteases including Escherichia coli RseP. Structure-based chemical modification and cross-linking experiments indicated that the RseP domains surrounding the active center undergo conformational changes to expose the substrate-binding site, suggesting that RseP has a gating mechanism to regulate substrate entry. Furthermore, mutational analysis suggests that a conserved electrostatic linkage between the transmembrane and peripheral membrane-associated domains mediates the conformational changes. In vivo cleavage assays also support that the substrate transmembrane helix is unwound by strand addition to the intramembrane beta sheet of RseP and is clamped by a conserved asparagine residue at the active center for efficient cleavage. This mechanism underlying the substrate binding, i.e., unwinding and clamping, appears common across distinct families of intramembrane proteases that cleave transmembrane segments.
		+
		+	Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP.,Imaizumi Y, Takanuki K, Miyake T, Takemoto M, Hirata K, Hirose M, Oi R, Kobayashi T, Miyoshi K, Aruga R, Yokoyama T, Katagiri S, Matsuura H, Iwasaki K, Kato T, Kaneko MK, Kato Y, Tajiri M, Akashi S, Nureki O, Hizukuri Y, Akiyama Y, Nogi T Sci Adv. 2022 Aug 26;8(34):eabp9011. doi: 10.1126/sciadv.abp9011. Epub 2022 Aug, 24. PMID:36001659<ref>PMID:36001659</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w6z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:39, 7 September 2022

OCA — Wed, 07 Sep 2022 16:39:24 GMT

←Older revision		Revision as of 16:39, 7 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w6z is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Kangiella koreensis RseP orthologue in complex with batimastat in space group P21==
-		+	<StructureSection load='7w6z' size='340' side='right'caption='[[7w6z]], [[Resolution\|resolution]] 3.15Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7w6z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kangiella_koreensis_DSM_16069 Kangiella koreensis DSM 16069]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W6Z FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BAT:4-(N-HYDROXYAMINO)-2R-ISOBUTYL-2S-(2-THIENYLTHIOMETHYL)SUCCINYL-L-PHENYLALANINE-N-METHYLAMIDE'>BAT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w6z OCA], [https://pdbe.org/7w6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w6z RCSB], [https://www.ebi.ac.uk/pdbsum/7w6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w6z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/C7R5Z1_KANKD C7R5Z1_KANKD]]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kangiella koreensis DSM 16069]]
		+	[[Category: Large Structures]]
		+	[[Category: Imaizumi Y]]
		+	[[Category: Nogi T]]
		+	[[Category: Takanuki K]]

OCA at 08:43, 12 January 2022

OCA — Wed, 12 Jan 2022 08:43:18 GMT

←Older revision		Revision as of 08:43, 12 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w6z is ON HOLD	+	The entry 7w6z is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7w6z" [edit=sysop:move=sysop]

OCA — Wed, 08 Dec 2021 08:48:41 GMT

Protected "7w6z" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:48, 8 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w6z is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Dec 2021 08:48:40 GMT

New page: '''Unreleased structure''' The entry 7w6z is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7w6z is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]