7w7c - Revision history

OCA at 17:37, 29 November 2023

OCA — Wed, 29 Nov 2023 17:37:20 GMT

←Older revision		Revision as of 17:37, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7w7c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae_NCTC_13129 Corynebacterium diphtheriae NCTC 13129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W7C FirstGlance]. <br>		<table><tr><td colspan='2'>[[7w7c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae_NCTC_13129 Corynebacterium diphtheriae NCTC 13129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W7C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7c OCA], [https://pdbe.org/7w7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7c RCSB], [https://www.ebi.ac.uk/pdbsum/7w7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7c OCA], [https://pdbe.org/7w7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7c RCSB], [https://www.ebi.ac.uk/pdbsum/7w7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7c ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:48, 11 January 2023

OCA — Wed, 11 Jan 2023 07:48:00 GMT

←Older revision		Revision as of 07:48, 11 January 2023
Line 1:		Line 1:

-	====	+	==Heme exporter in the unliganded form==
-	<StructureSection load='7w7c' size='340' side='right'caption='[[7w7c]]' scene=''>	+	<StructureSection load='7w7c' size='340' side='right'caption='[[7w7c]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7w7c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae_NCTC_13129 Corynebacterium diphtheriae NCTC 13129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W7C FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7c OCA], [https://pdbe.org/7w7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7c RCSB], [https://www.ebi.ac.uk/pdbsum/7w7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7c ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7c OCA], [https://pdbe.org/7w7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7c RCSB], [https://www.ebi.ac.uk/pdbsum/7w7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6NEF2_CORDI Q6NEF2_CORDI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial pathogens acquire heme from the host hemoglobin as an iron nutrient for their virulence and proliferation in blood. Concurrently, they encounter cytotoxic-free heme that escapes the heme-acquisition process. To overcome this toxicity, many gram-positive bacteria employ an ATP-binding cassette heme-dedicated efflux pump, HrtBA in the cytoplasmic membranes. Although genetic analyses have suggested that HrtBA expels heme from the bacterial membranes, the molecular mechanism of heme efflux remains elusive due to the lack of protein studies. Here, we show the biochemical properties and crystal structures of Corynebacterium diphtheriae HrtBA, alone and in complex with heme or an ATP analog, and we reveal how HrtBA extracts heme from the membrane and releases it. HrtBA consists of two cytoplasmic HrtA ATPase subunits and two transmembrane HrtB permease subunits. A heme-binding site is formed in the HrtB dimer and is laterally accessible to heme in the outer leaflet of the membrane. The heme-binding site captures heme from the membrane using a glutamate residue of either subunit as an axial ligand and sequesters the heme within the rearranged transmembrane helix bundle. By ATP-driven HrtA dimerization, the heme-binding site is squeezed to extrude the bound heme. The mechanism sheds light on the detoxification of membrane-bound heme in this bacterium.
		+
		+	Structural basis for heme detoxification by an ATP-binding cassette-type efflux pump in gram-positive pathogenic bacteria.,Nakamura H, Hisano T, Rahman MM, Tosha T, Shirouzu M, Shiro Y Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2123385119. doi: , 10.1073/pnas.2123385119. Epub 2022 Jun 29. PMID:35767641<ref>PMID:35767641</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w7c" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Corynebacterium diphtheriae NCTC 13129]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hisano T]]
		+	[[Category: Nakamura H]]
		+	[[Category: Rahman MM]]
		+	[[Category: Shiro Y]]
		+	[[Category: Shirouzu M]]
		+	[[Category: Tosha T]]

OCA at 09:47, 22 June 2022

OCA — Wed, 22 Jun 2022 09:47:08 GMT

←Older revision		Revision as of 09:47, 22 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w7c ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7w7c' size='340' side='right'caption='[[7w7c]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7c OCA], [https://pdbe.org/7w7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7c RCSB], [https://www.ebi.ac.uk/pdbsum/7w7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7c ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:43, 12 January 2022

OCA — Wed, 12 Jan 2022 08:43:39 GMT

←Older revision		Revision as of 08:43, 12 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w7c is ON HOLD	+	The entry 7w7c is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7w7c" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:48:18 GMT

Protected "7w7c" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:48, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w7c is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Dec 2021 15:48:17 GMT

New page: '''Unreleased structure''' The entry 7w7c is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7w7c is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]