7w81 - Revision history

OCA at 17:37, 29 November 2023

OCA — Wed, 29 Nov 2023 17:37:38 GMT

←Older revision		Revision as of 17:37, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7w81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W81 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7w81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W81 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w81 OCA], [https://pdbe.org/7w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w81 RCSB], [https://www.ebi.ac.uk/pdbsum/7w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w81 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w81 OCA], [https://pdbe.org/7w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w81 RCSB], [https://www.ebi.ac.uk/pdbsum/7w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w81 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 7w81" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7w81" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Haptoglobin receptor\|Haptoglobin receptor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:23, 19 October 2022

OCA — Wed, 19 Oct 2022 19:23:08 GMT

←Older revision		Revision as of 19:23, 19 October 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w81 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the heme-bound form of the linker-NEAT3 region of IsdH from Staphylococcus aureus==
		+	<StructureSection load='7w81' size='340' side='right'caption='[[7w81]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7w81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W81 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w81 OCA], [https://pdbe.org/7w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w81 RCSB], [https://www.ebi.ac.uk/pdbsum/7w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w81 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus is a major cause of deadly nosocomial infections, a severe problem fueled by the steady increase of resistant bacteria. The iron surface determinant (Isd) system is a family of proteins that acquire nutritional iron from the host organism, helping the bacterium to proliferate during infection, and therefore represents a promising antibacterial target. In particular, the surface protein IsdH captures hemoglobin (Hb) and acquires the heme moiety containing the iron atom. Structurally, IsdH comprises three distinctive NEAr-iron Transporter (NEAT) domains connected by linker domains. The objective of this study was to characterize the linker region between NEAT2 and NEAT3 from various biophysical viewpoints and thereby advance our understanding of its role in the molecular mechanism of heme extraction. We demonstrate the linker region contributes to the stability of the bound protein, likely influencing the flexibility and orientation of the NEAT3 domain in its interaction with Hb, but only exerts a modest contribution to the affinity of IsdH for heme. Based on these data, we suggest that the flexible nature of the linker facilitates the precise positioning of NEAT3 to acquire heme. In addition, we also found that residues His45 and His89 of Hb located in the heme transfer route toward IsdH do not play a critical role in the transfer rate-determining step. In conclusion, this study clarifies key elements of the mechanism of heme extraction of human Hb by IsdH, providing key insights into the Isd system and other protein systems containing NEAT domains.

-	~~Authors~~:	+	Structure and role of the linker domain of the iron surface-determinant protein IsdH in heme transportation in Staphylococcus aureus.,Valenciano-Bellido S, Caaveiro JMM, Morante K, Sushko T, Nakakido M, Nagatoishi S, Tsumoto K J Biol Chem. 2022 Jun;298(6):101995. doi: 10.1016/j.jbc.2022.101995. Epub 2022, Apr 29. PMID:35500652<ref>PMID:35500652</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7w81" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
		+	[[Category: Caaveiro JMM]]
		+	[[Category: Tsumoto K]]
		+	[[Category: Vu N]]

OCA at 12:35, 29 December 2021

OCA — Wed, 29 Dec 2021 12:35:49 GMT

←Older revision		Revision as of 12:35, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w81 is ON HOLD	+	The entry 7w81 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7w81" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:49:42 GMT

Protected "7w81" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:49, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w81 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Dec 2021 15:49:41 GMT

New page: '''Unreleased structure''' The entry 7w81 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7w81 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]