7w8n - Revision history

OCA at 17:37, 29 November 2023

OCA — Wed, 29 Nov 2023 17:37:50 GMT

←Older revision		Revision as of 17:37, 29 November 2023
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-	====	+	==Microbial Hormone-sensitive lipase E53 wild type==
-	<StructureSection load='7w8n' size='340' side='right'caption='[[7w8n]]' scene=''>	+	<StructureSection load='7w8n' size='340' side='right'caption='[[7w8n]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7w8n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrobacter_longus Erythrobacter longus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W8N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8n OCA], [https://pdbe.org/7w8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8n RCSB], [https://www.ebi.ac.uk/pdbsum/7w8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8n ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6NA:HEXANOIC+ACID'>6NA</scene>, <scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene>, <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>, <scene name='pdbligand=D8F:(4-nitrophenyl)+hexanoate'>D8F</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8n OCA], [https://pdbe.org/7w8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8n RCSB], [https://www.ebi.ac.uk/pdbsum/7w8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8n ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A074MDU6_ERYLO A0A074MDU6_ERYLO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Esterases are a class of enzymes that split esters into an acid and an alcohol in a chemical reaction with water, having high potential in pharmaceutical, food and biofuel industrial applications. To advance the understanding of esterases, we have identified and characterized E53, an alkalophilic esterase from a marine bacterium Erythrobacter longus. The crystal structures of wild type E53 and three variants were solved successfully using the X-ray diffraction method. Phylogenetic analysis classified E53 as a member of the family IV esterase. The enzyme showed highest activity against p-nitrophenyl butyrate substrate at pH 8.5-9.5 and 40( degrees )C. Based on the structural feature, the catalytic pocket was defined as R1 (catalytic center), R2 (pocket entrance), and R3 (end area of pocket) regions. Nine variants were generated spanning R1-R3 and thorough functional studies were performed. Detailed structural analysis and the results obtained from the mutagenesis study revealed that mutations in the R1 region could regulate the catalytic reaction in both positive and negative directions; expanding the bottleneck in R2 region has improved the enzymatic activity; and R3 region was associated with the determination of the pH pattern of E53. N166A in R3 region showed reduced activity only under alkaline conditions, and structural analysis indicated the role of N166 in stabilizing the loop by forming a hydrogen bond with L193 and G233. In summary, the systematic studies on E53 performed in this work provide structural and functional insights into alkaliphilic esterases and further our knowledge of these enzymes.
		+
		+	Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated From Erythrobacter longus.,Ding Y, Nie L, Yang XC, Li Y, Huo YY, Li Z, Gao Y, Cui HL, Li J, Xu XW Front Microbiol. 2022 Jan 5;12:798194. doi: 10.3389/fmicb.2021.798194., eCollection 2021. PMID:35069500<ref>PMID:35069500</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w8n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Erythrobacter longus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Li J]]
		+	[[Category: Li Z]]
		+	[[Category: Xu X]]
		+	[[Category: Yang X]]

OCA at 07:45, 23 February 2022

OCA — Wed, 23 Feb 2022 07:45:26 GMT

←Older revision		Revision as of 07:45, 23 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w8n ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7w8n' size='340' side='right'caption='[[7w8n]]' scene=''>
-	~~Authors~~: ~~Yang, X~~.~~, Li, Z~~.~~, Xu, X~~.~~, Li, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Microbial Hormone-sensitive lipase E53 wild type~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8n OCA], [https://pdbe.org/7w8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8n RCSB], [https://www.ebi.ac.uk/pdbsum/7w8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8n ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Yang~~, X]]	+	__TOC__
-	[~~[Category~~: Li, Z]]	+	</StructureSection>
-	[[Category: ~~Li, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Xu, X~~]]	+	[[Category: Z-disk]]

OCA at 09:24, 5 January 2022

OCA — Wed, 05 Jan 2022 09:24:09 GMT

←Older revision		Revision as of 09:24, 5 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w8n is ON HOLD	+	The entry 7w8n is ON HOLD until Paper Publication

	Authors: Yang, X., Li, Z., Xu, X., Li, J.		Authors: Yang, X., Li, Z., Xu, X., Li, J.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yang, X]]		[[Category: Yang, X]]
-	[[Category: Li, J]]
	[[Category: Li, Z]]		[[Category: Li, Z]]
		+	[[Category: Li, J]]
	[[Category: Xu, X]]		[[Category: Xu, X]]

OCA: Protected "7w8n" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:51:06 GMT

Protected "7w8n" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:51, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w8n is ON HOLD Authors: Yang, X., Li, Z., Xu, X., Li, J. Description: Microbial Hormone-sensitive lipase E53 wild type [[Category: Unreleased Stru...

OCA — Wed, 15 Dec 2021 15:51:04 GMT

New page: '''Unreleased structure''' The entry 7w8n is ON HOLD Authors: Yang, X., Li, Z., Xu, X., Li, J. Description: Microbial Hormone-sensitive lipase E53 wild type [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 7w8n is ON HOLD

Authors: Yang, X., Li, Z., Xu, X., Li, J.

Description: Microbial Hormone-sensitive lipase E53 wild type
[[Category: Unreleased Structures]]
[[Category: Yang, X]]
[[Category: Li, J]]
[[Category: Li, Z]]
[[Category: Xu, X]]