OCA at 20:39, 12 April 2023

OCA — Wed, 12 Apr 2023 20:39:07 GMT

←Older revision		Revision as of 20:39, 12 April 2023
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-	====	+	==Structure of SARS-CoV-2 spike receptor-binding domain Y453F mutation complexed with American mink ACE2==
-	<StructureSection load='7w8s' size='340' side='right'caption='[[7w8s]]' scene=''>	+	<StructureSection load='7w8s' size='340' side='right'caption='[[7w8s]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7w8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W8S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8s OCA], [https://pdbe.org/7w8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8s RCSB], [https://www.ebi.ac.uk/pdbsum/7w8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8s ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8s OCA], [https://pdbe.org/7w8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8s RCSB], [https://www.ebi.ac.uk/pdbsum/7w8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A8C7BTF2_NEOVI A0A8C7BTF2_NEOVI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted between humans and minks, and some mutations in the spike (S) protein, especially in the receptor-binding domain (RBD), have been identified in mink-derived viruses. Here, we examined binding of the mink angiotensin-converting enzyme 2 (ACE2) receptor to mink-derived and important human-originating variants, and we demonstrated that most of the RBD variants increased the binding affinities to mink ACE2 (mkACE2). Cryo-electron microscopy structures of the mkACE2-RBD Y453F (with a Y-to-F change at position 453) and mkACE2-RBD F486L complexes helped identify the key residues that facilitate changes in mkACE2 binding affinity. Additionally, the data indicated that the Y453F and F486L mutations reduced the binding affinities to some human monoclonal antibodies, and human vaccinated sera efficiently prevented infection of human cells by pseudoviruses expressing Y453F, F486L, or N501T RBD. Our findings provide an important molecular mechanism for the rapid adaptation of SARS-CoV-2 in minks and highlight the potential influence of the main mink-originating variants for humans. IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a broad range of hosts. Mink-derived SARS-CoV-2 can transmit back to humans. There is an urgent need to understand the binding mechanism of mink-derived SARS-CoV-2 variants to mink receptor. In this study, we identified all mutations in the receptor-binding domain (RBD) of spike (S) protein from mink-derived SARS-CoV-2, and we demonstrated the enhanced binding affinity of mink angiotensin-converting enzyme 2 (ACE2) to most of the mink-derived RBD variants as well as important human-originating RBD variants. Cryo-electron microscopy structures revealed that the Y453F and F486L mutations enhanced the binding forces in the interaction interface. In addition, Y453F and F486L mutations reduced the binding affinities to some human monoclonal antibodies, and the SARS-CoV-2 pseudoviruses with Y453F, F486L, or N501T mutations were neutralized by human vaccinated sera. Therefore, our results provide valuable information for understanding the cross-species transmission mechanism of SARS-CoV-2.
		+
		+	Molecular Basis of Mink ACE2 Binding to SARS-CoV-2 and Its Mink-Derived Variants.,Su C, He J, Han P, Bai B, Li D, Cao J, Tian M, Hu Y, Zheng A, Niu S, Chen Q, Rong X, Zhang Y, Li W, Qi J, Zhao X, Yang M, Wang Q, Gao GF J Virol. 2022 Sep 14;96(17):e0081422. doi: 10.1128/jvi.00814-22. Epub 2022 Aug , 24. PMID:36000849<ref>PMID:36000849</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w8s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Gao GF]]
		+	[[Category: Qi JX]]
		+	[[Category: Su C]]

OCA at 06:23, 18 August 2022

OCA — Thu, 18 Aug 2022 06:23:18 GMT

←Older revision		Revision as of 06:23, 18 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w8s ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7w8s' size='340' side='right'caption='[[7w8s]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w8s OCA], [https://pdbe.org/7w8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w8s RCSB], [https://www.ebi.ac.uk/pdbsum/7w8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w8s ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 06:07, 19 January 2022

OCA — Wed, 19 Jan 2022 06:07:33 GMT

←Older revision		Revision as of 06:07, 19 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w8s is ON HOLD	+	The entry 7w8s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7w8s" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:51:18 GMT

Protected "7w8s" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:51, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w8s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Dec 2021 15:51:17 GMT

New page: '''Unreleased structure''' The entry 7w8s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7w8s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7w8s - Revision history

OCA at 20:39, 12 April 2023

OCA at 06:23, 18 August 2022

OCA at 06:07, 19 January 2022

OCA: Protected "7w8s" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7w8s is ON HOLD Authors: Description: Category: Unreleased Structures