OCA at 08:56, 28 December 2022

OCA — Wed, 28 Dec 2022 08:56:36 GMT

←Older revision		Revision as of 08:56, 28 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wbb is ~~ON HOLD~~	+	==Cryo-EM structure of substrate engaged Drg1 hexamer==
		+	<StructureSection load='7wbb' size='340' side='right'caption='[[7wbb]], [[Resolution\|resolution]] 3.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7wbb]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WBB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wbb OCA], [https://pdbe.org/7wbb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wbb RCSB], [https://www.ebi.ac.uk/pdbsum/7wbb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wbb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6A5PRU8_YEASX A0A6A5PRU8_YEASX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The type II AAA + ATPase Drg1 is a ribosome assembly factor, functioning to release Rlp24 from the pre-60S particle just exported from nucleus, and its activity in can be inhibited by a drug molecule diazaborine. However, molecular mechanisms of Drg1-mediated Rlp24 removal and diazaborine-mediated inhibition are not fully understood. Here, we report Drg1 structures in different nucleotide-binding and benzo-diazaborine treated states. Drg1 hexamers transits between two extreme conformations (planar or helical arrangement of protomers). By forming covalent adducts with ATP molecules in both ATPase domain, benzo-diazaborine locks Drg1 hexamers in a symmetric and non-productive conformation to inhibits both inter-protomer and inter-ring communication of Drg1 hexamers. We also obtained a substrate-engaged mutant Drg1 structure, in which conserved pore-loops form a spiral staircase to interact with the polypeptide through a sequence-independent manner. Structure-based mutagenesis data highlight the functional importance of the pore-loop, the D1-D2 linker and the inter-subunit signaling motif of Drg1, which share similar regulatory mechanisms with p97. Our results suggest that Drg1 may function as an unfoldase that threads a substrate protein within the pre-60S particle.

-	~~Authors~~:	+	Structural dynamics of AAA + ATPase Drg1 and mechanism of benzo-diazaborine inhibition.,Ma C, Wu D, Chen Q, Gao N Nat Commun. 2022 Nov 9;13(1):6765. doi: 10.1038/s41467-022-34511-2. PMID:36351914<ref>PMID:36351914</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7wbb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Chen Q]]
		+	[[Category: Gao N]]
		+	[[Category: Ma CY]]
		+	[[Category: Wu DM]]

OCA: Protected "7wbb" [edit=sysop:move=sysop]

OCA — Wed, 22 Dec 2021 14:17:12 GMT

Protected "7wbb" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:17, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7wbb is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Dec 2021 14:17:11 GMT

New page: '''Unreleased structure''' The entry 7wbb is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7wbb is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7wbb - Revision history

OCA at 08:56, 28 December 2022

OCA: Protected "7wbb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7wbb is ON HOLD Authors: Description: Category: Unreleased Structures