OCA at 05:36, 5 July 2023

OCA — Wed, 05 Jul 2023 05:36:34 GMT

←Older revision		Revision as of 05:36, 5 July 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7wbv is ~~ON HOLD until 2024~~-06-17	+	==RNA polymerase II elongation complex bound with Elf1 and Spt4/5, stalled at SHL(-4) of the nucleosome==
		+	<StructureSection load='7wbv' size='340' side='right'caption='[[7wbv]], [[Resolution\|resolution]] 4.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7wbv]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii Komagataella phaffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WBV FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wbv OCA], [https://pdbe.org/7wbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wbv RCSB], [https://www.ebi.ac.uk/pdbsum/7wbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wbv ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In transcription-coupled repair (TCR), transcribing RNA polymerase II (RNAPII) stalls at a DNA lesion and recruits TCR proteins to the damaged site. However, the mechanism by which RNAPII recognizes a DNA lesion in the nucleosome remains enigmatic. In the present study, we inserted an apurinic/apyrimidinic DNA lesion analogue, tetrahydrofuran (THF), in the nucleosomal DNA, where RNAPII stalls at the SHL(-4), SHL(-3.5), and SHL(-3) positions, and determined the structures of these complexes by cryo-electron microscopy. In the RNAPII-nucleosome complex stalled at SHL(-3.5), the nucleosome orientation relative to RNAPII is quite different from those in the SHL(-4) and SHL(-3) complexes, which have nucleosome orientations similar to naturally paused RNAPII-nucleosome complexes. Furthermore, we found that an essential TCR protein, Rad26 (CSB), enhances the RNAPII processivity, and consequently augments the DNA damage recognition efficiency of RNAPII in the nucleosome. The cryo-EM structure of the Rad26-RNAPII-nucleosome complex revealed that Rad26 binds to the stalled RNAPII through a novel interface, which is completely different from those previously reported. These structures may provide important information to understand the mechanism by which RNAPII recognizes the nucleosomal DNA lesion and recruits TCR proteins to the stalled RNAPII on the nucleosome.

-	~~Authors~~:	+	Structural Basis of Damaged Nucleotide Recognition by Transcribing RNA Polymerase II in the Nucleosome.,Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H J Mol Biol. 2023 Jul 1;435(13):168130. doi: 10.1016/j.jmb.2023.168130. Epub 2023 , Apr 28. PMID:37120012<ref>PMID:37120012</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7wbv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Komagataella phaffii]]
		+	[[Category: Large Structures]]
		+	[[Category: Ehara H]]
		+	[[Category: Kujirai T]]
		+	[[Category: Kurumizaka H]]
		+	[[Category: Osumi K]]
		+	[[Category: Sekine S]]
		+	[[Category: Takizawa Y]]

OCA at 07:07, 14 December 2022

OCA — Wed, 14 Dec 2022 07:07:24 GMT

←Older revision		Revision as of 07:07, 14 December 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wbv is ON HOLD until ~~Paper Publication~~	+	The entry 7wbv is ON HOLD until 2024-06-17

	Authors:		Authors:

OCA at 05:41, 27 January 2022

OCA — Thu, 27 Jan 2022 05:41:18 GMT

←Older revision		Revision as of 05:41, 27 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wbv is ON HOLD	+	The entry 7wbv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7wbv" [edit=sysop:move=sysop]

OCA — Wed, 22 Dec 2021 14:18:50 GMT

Protected "7wbv" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:18, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7wbv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Dec 2021 14:18:49 GMT

New page: '''Unreleased structure''' The entry 7wbv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7wbv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7wbv - Revision history

OCA at 05:36, 5 July 2023

OCA at 07:07, 14 December 2022

OCA at 05:41, 27 January 2022

OCA: Protected "7wbv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7wbv is ON HOLD Authors: Description: Category: Unreleased Structures