7wcg - Revision history

OCA at 11:21, 14 June 2023

OCA — Wed, 14 Jun 2023 11:21:58 GMT

←Older revision		Revision as of 11:21, 14 June 2023
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	==Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature==		==Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature==
-	<StructureSection load='7wcg' size='340' side='right'caption='[[7wcg~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7wcg' size='340' side='right'caption='[[7wcg]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7wcg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wcg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcg OCA], [https://pdbe.org/7wcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcg RCSB], [https://www.ebi.ac.uk/pdbsum/7wcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcg OCA], [https://pdbe.org/7wcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcg RCSB], [https://www.ebi.ac.uk/pdbsum/7wcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2]] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>	+	[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lee, W~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: ~~Park, C~~]]	+	[[Category: Lee W]]
-	[[Category: ~~Yang, M J~~]]	+	[[Category: Park C]]
-	[[Category: ~~Dna binding protein]]~~	+	[[Category: Yang MJ]]
-	~~[[Category: Hyperthermophilic]]~~	+
-	~~[[Category: Ob-fold]]~~	+
-	~~[[Category: Structure at high-temperature~~]]	+

OCA at 05:23, 15 June 2022

OCA — Wed, 15 Jun 2022 05:23:56 GMT

←Older revision		Revision as of 05:23, 15 June 2022
Line 1:		Line 1:

	==Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature==		==Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature==
-	<StructureSection load='7wcg' size='340' side='right'caption='[[7wcg]]' scene=''>	+	<StructureSection load='7wcg' size='340' side='right'caption='[[7wcg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wcg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcg OCA], [https://pdbe.org/7wcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcg RCSB], [https://www.ebi.ac.uk/pdbsum/7wcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcg OCA], [https://pdbe.org/7wcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcg RCSB], [https://www.ebi.ac.uk/pdbsum/7wcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2]] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins from Sulfolobus solfataricus (S. solfataricus), an extremophile, are active even at high temperatures. The single-stranded DNA (ssDNA) binding protein of S. solfataricus (SsoSSB) is overexpressed to protect ssDNA during DNA metabolism. Although SsoSSB has the potential to be applied in various areas, its structural and ssDNA binding properties at high temperatures have not been studied. We present the solution structure, backbone dynamics, and ssDNA binding properties of SsoSSB at 50 degrees C. The overall structure is consistent with the structures previously studied at room temperature. However, the loop between the first two beta sheets, which is flexible and is expected to undergo conformational change upon ssDNA binding, shows a difference from the ssDNA bound structure. The ssDNA binding ability was maintained at high temperature, but different interactions were observed depending on the temperature. Backbone dynamics at high temperature showed that the rigidity of the structured region was well maintained. The investigation of an N-terminal deletion mutant revealed that it is important for maintaining thermostability, structure, and ssDNA binding ability. The structural and dynamic properties of SsoSSB observed at high temperature can provide information on the behavior of proteins in thermophiles at the molecular level and guide the development of new experimental techniques.
		+
		+	NMR Structure and Biophysical Characterization of Thermophilic Single-Stranded DNA Binding Protein from Sulfolobus Solfataricus.,Yang MJ, Kim J, Lee Y, Lee W, Park CJ Int J Mol Sci. 2022 Mar 13;23(6). pii: ijms23063099. doi: 10.3390/ijms23063099. PMID:35328522<ref>PMID:35328522</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wcg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lee W]]	+	[[Category: Lee, W]]
-	[[Category: Park C]]	+	[[Category: Park, C]]
-	[[Category: Yang MJ]]	+	[[Category: Yang, M J]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Hyperthermophilic]]
		+	[[Category: Ob-fold]]
		+	[[Category: Structure at high-temperature]]

OCA at 05:31, 8 June 2022

OCA — Wed, 08 Jun 2022 05:31:38 GMT

←Older revision		Revision as of 05:31, 8 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wcg ~~is ON HOLD until Paper Publication~~	+	==Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature==
-		+	<StructureSection load='7wcg' size='340' side='right'caption='[[7wcg]]' scene=''>
-	~~Authors~~: ~~Yang~~, M.J., ~~Park~~, C., ~~Lee~~, W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCG FirstGlance]. <br>
-	~~Description: Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcg OCA], [https://pdbe.org/7wcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcg RCSB], [https://www.ebi.ac.uk/pdbsum/7wcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Lee, W]]	+	__TOC__
-	[[Category: Park, C]]	+	</StructureSection>
-	[[Category: Yang~~, M.J~~]]	+	[[Category: Large Structures]]
		+	[[Category: Lee W]]
		+	[[Category: Park C]]
		+	[[Category: Yang MJ]]

OCA at 05:41, 27 January 2022

OCA — Thu, 27 Jan 2022 05:41:34 GMT

←Older revision		Revision as of 05:41, 27 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wcg is ON HOLD	+	The entry 7wcg is ON HOLD until Paper Publication

	Authors: Yang, M.J., Park, C., Lee, W.		Authors: Yang, M.J., Park, C., Lee, W.

OCA: Protected "7wcg" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2021 12:38:39 GMT

Protected "7wcg" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:38, 29 December 2021

OCA: New page: '''Unreleased structure''' The entry 7wcg is ON HOLD Authors: Yang, M.J., Park, C., Lee, W. Description: Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at hig...

OCA — Wed, 29 Dec 2021 12:38:37 GMT

New page: '''Unreleased structure''' The entry 7wcg is ON HOLD Authors: Yang, M.J., Park, C., Lee, W. Description: Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at hig...

New page

'''Unreleased structure'''

The entry 7wcg is ON HOLD

Authors: Yang, M.J., Park, C., Lee, W.

Description: Single-Stranded DNA binding protein of Sulfolobus Solfataricus structure at high-temperature
[[Category: Unreleased Structures]]
[[Category: Lee, W]]
[[Category: Park, C]]
[[Category: Yang, M.J]]