OCA at 17:39, 29 November 2023

OCA — Wed, 29 Nov 2023 17:39:57 GMT

←Older revision		Revision as of 17:39, 29 November 2023
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-	==~~n/a~~==	+	==Fungal immunomodulatory protein FIP-nha==
-	<StructureSection load='7wdl' size='340' side='right'caption='[[7wdl]]' scene=''>	+	<StructureSection load='7wdl' size='340' side='right'caption='[[7wdl]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WDL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wdl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_solani Fusarium solani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WDL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wdl OCA], [https://pdbe.org/7wdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wdl RCSB], [https://www.ebi.ac.uk/pdbsum/7wdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wdl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.903Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wdl OCA], [https://pdbe.org/7wdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wdl RCSB], [https://www.ebi.ac.uk/pdbsum/7wdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wdl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C7ZE17_FUSV7 C7ZE17_FUSV7]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fungal immunomodulatory proteins (FIPs) have been investigated for their use as potential natural derived anti-tumor molecules. However, the stability of FIPs is critical for their preparation and storage. In this study, the correlation between thermal stability and protein structural features of rFIP-nha, with significant anti-tumor activity, has been evaluated. For comprehensive analysis, FIP-nha and its homologues FIP-gmi, FIP-fve, and LZ-8 were all recombinantly expressed in E. coli. In solution, rFIP-nha and rFIP-gmi formed tetramers; rFIP-fve and rLZ-8 appeared as dimers. Their melting temperatures were 85.1 degrees C, 77.8 degrees C, 66.5 degrees C, and 64.4 degrees C, respectively. Accordingly, their cytotoxicity was also temperature dependent. To investigate the underlying mechanism of their thermostability, we solved the crystal structure of FIP-nha. Detailed structure analysis, molecular dynamic simulation and mutagenesis studies indicated that a higher thermostability was correlated to higher oligomerization states, larger interface area, and more interactions. The structure property studies indicate that Y12, D61 and Y108 were critical for oligomerization and high thermostability of rFIP-nha, but the dimeric and tetrameric states of rFIP-nha exert similar cytotoxicity on A549 cells. Taken together, these findings reveal that thermostability of FIPs was dependent on their oligomerization state, and correlated with their cytotoxicity.
		+
		+	Linking the thermostability of FIP-nha (Nectria haematococca) to its structural properties.,Liu Y, Bastiaan-Net S, Zhang Y, Hoppenbrouwers T, Xie Y, Wang Y, Wei X, Du G, Zhang H, Imam KMDSU, Wichers H, Li Z Int J Biol Macromol. 2022 Jul 31;213:555-564. doi: , 10.1016/j.ijbiomac.2022.05.136. Epub 2022 May 26. PMID:35644318<ref>PMID:35644318</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wdl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium solani]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~N/a~~]]	+	[[Category: Bastiaan-Net S]]
		+	[[Category: Du G]]
		+	[[Category: Hoppenbrouwers T]]
		+	[[Category: Imam KMSU]]
		+	[[Category: Li Z]]
		+	[[Category: Liu Y]]
		+	[[Category: Wang Y]]
		+	[[Category: Wei X]]
		+	[[Category: Wichers HJ]]
		+	[[Category: Xie Y]]
		+	[[Category: Zhang H]]
		+	[[Category: Zhang Y]]

OCA at 07:28, 29 June 2022

OCA — Wed, 29 Jun 2022 07:28:31 GMT

←Older revision		Revision as of 07:28, 29 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wdl ~~is ON HOLD until Paper Publication~~	+	==n/a==
-		+	<StructureSection load='7wdl' size='340' side='right'caption='[[7wdl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WDL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wdl OCA], [https://pdbe.org/7wdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wdl RCSB], [https://www.ebi.ac.uk/pdbsum/7wdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wdl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: N/a]]

OCA at 05:45, 2 February 2022

OCA — Wed, 02 Feb 2022 05:45:23 GMT

←Older revision		Revision as of 05:45, 2 February 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wdl is ON HOLD	+	The entry 7wdl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7wdl" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2021 12:41:31 GMT

Protected "7wdl" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:41, 29 December 2021

OCA: New page: '''Unreleased structure''' The entry 7wdl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Dec 2021 12:41:29 GMT

New page: '''Unreleased structure''' The entry 7wdl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7wdl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7wdl - Revision history

OCA at 17:39, 29 November 2023

OCA at 07:28, 29 June 2022

OCA at 05:45, 2 February 2022

OCA: Protected "7wdl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7wdl is ON HOLD Authors: Description: Category: Unreleased Structures