7wqi - Revision history

OCA at 17:43, 29 November 2023

OCA — Wed, 29 Nov 2023 17:43:36 GMT

←Older revision		Revision as of 17:43, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7wqi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WQI FirstGlance]. <br>		<table><tr><td colspan='2'>[[7wqi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WQI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=80I:[(3~{S})-3-[[(2~{S})-2-[(4-methoxy-1~{H}-indol-2-yl)carbonylamino]-4-methyl-pentanoyl]amino]-2-oxidanylidene-4-[(3~{R})-2-oxidanylidene-3,4-dihydropyrrol-3-yl]butyl]+dihydrogen+phosphate'>80I</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=80I:[(3~{S})-3-[[(2~{S})-2-[(4-methoxy-1~{H}-indol-2-yl)carbonylamino]-4-methyl-pentanoyl]amino]-2-oxidanylidene-4-[(3~{R})-2-oxidanylidene-3,4-dihydropyrrol-3-yl]butyl]+dihydrogen+phosphate'>80I</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wqi OCA], [https://pdbe.org/7wqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wqi RCSB], [https://www.ebi.ac.uk/pdbsum/7wqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wqi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wqi OCA], [https://pdbe.org/7wqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wqi RCSB], [https://www.ebi.ac.uk/pdbsum/7wqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wqi ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:32, 25 January 2023

OCA — Wed, 25 Jan 2023 06:32:30 GMT

←Older revision		Revision as of 06:32, 25 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7wqi is ~~ON HOLD until 2024~~-01-25	+	==Crystal structure of SARS coronavirus main protease in complex with PF07304814==
-		+	<StructureSection load='7wqi' size='340' side='right'caption='[[7wqi]], [[Resolution\|resolution]] 1.93Å' scene=''>
-	~~Authors~~: ~~Lin~~, C., ~~Zhong~~, F.L., ~~Zhou~~, X.L., ~~Zeng~~, P., ~~Zhang~~, J., Li, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7wqi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WQI FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~SARS coronavirus main protease~~ in ~~complex~~ with ~~PF07304814~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=80I:[(3~{S})-3-[[(2~{S})-2-[(4-methoxy-1~{H}-indol-2-yl)carbonylamino]-4-methyl-pentanoyl]amino]-2-oxidanylidene-4-[(3~{R})-2-oxidanylidene-3,4-dihydropyrrol-3-yl]butyl]+dihydrogen+phosphate'>80I</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wqi OCA], [https://pdbe.org/7wqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wqi RCSB], [https://www.ebi.ac.uk/pdbsum/7wqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wqi ProSAT]</span></td></tr>
-	[[Category: ~~Lin, C~~]]	+	</table>
-	[[Category: Li, J]]	+	== Function ==
-	[[Category: ~~Zhong, F.L~~]]	+	[https://www.uniprot.org/uniprot/R1A_SARS R1A_SARS] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein. Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (PubMed:23035226). May disrupt nuclear pore function by binding and displacing host NUP93 (PubMed:30943371).<ref>PMID:23035226</ref> <ref>PMID:30943371</ref> May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.<ref>PMID:19640993</ref> Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates (PubMed:17692280). Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3 (PubMed:19369340, PubMed:24622840). Prevents also host NF-kappa-B signaling.<ref>PMID:16271890</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> <ref>PMID:24622840</ref> <ref>PMID:24410069</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Alone appears incapable to induce membrane curvature, but together with nsp3 is able to induce paired membranes. Nsp3, nsp4 and nsp6 together are sufficient to form DMV.<ref>PMID:23943763</ref> <ref>PMID:24410069</ref> Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP). May cleave host ATP6V1G1 thereby modifying host vacuoles intracellular pH.[PROSITE-ProRule:PRU00772]<ref>PMID:16226257</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes (PubMed:24991833).<ref>PMID:24991833</ref> <ref>PMID:24410069</ref> Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> May participate in viral replication by acting as a ssRNA-binding protein.<ref>PMID:19153232</ref> Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.<ref>PMID:22635272</ref>
-	[[Category: ~~Zhou, X.L~~]]	+	== References ==
-	[[Category: ~~Zeng, P~~]]	+	<references/>
-	[[Category: ~~Zhang, J~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome-related coronavirus]]
		+	[[Category: Li J]]
		+	[[Category: Lin C]]
		+	[[Category: Zeng P]]
		+	[[Category: Zhang J]]
		+	[[Category: Zhong FL]]
		+	[[Category: Zhou XL]]

OCA at 06:54, 22 June 2022

OCA — Wed, 22 Jun 2022 06:54:16 GMT

←Older revision		Revision as of 06:54, 22 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wqi is ON HOLD until ~~Jan 25~~ 2024	+	The entry 7wqi is ON HOLD until 2024-01-25

	Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J.		Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J.

OCA at 05:01, 9 March 2022

OCA — Wed, 09 Mar 2022 05:01:40 GMT

←Older revision		Revision as of 05:01, 9 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wqi is ON HOLD	+	The entry 7wqi is ON HOLD until Jan 25 2024

	Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J.		Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J.

OCA: Protected "7wqi" [edit=sysop:move=sysop]

OCA — Wed, 02 Feb 2022 05:50:39 GMT

Protected "7wqi" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 2 February 2022

OCA: New page: '''Unreleased structure''' The entry 7wqi is ON HOLD Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J. Description: Crystal structure of SARS coronavirus main prot...

OCA — Wed, 02 Feb 2022 05:50:38 GMT

New page: '''Unreleased structure''' The entry 7wqi is ON HOLD Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J. Description: Crystal structure of SARS coronavirus main prot...

New page

'''Unreleased structure'''

The entry 7wqi is ON HOLD

Authors: Lin, C., Zhong, F.L., Zhou, X.L., Zeng, P., Zhang, J., Li, J.

Description: Crystal structure of SARS coronavirus main protease in complex with PF07304814
[[Category: Unreleased Structures]]
[[Category: Lin, C]]
[[Category: Li, J]]
[[Category: Zhong, F.L]]
[[Category: Zhou, X.L]]
[[Category: Zeng, P]]
[[Category: Zhang, J]]