7wtc - Revision history

OCA at 07:26, 9 November 2022

OCA — Wed, 09 Nov 2022 07:26:16 GMT

←Older revision		Revision as of 07:26, 9 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7wtc is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state==
		+	<StructureSection load='7wtc' size='340' side='right'caption='[[7wtc]], [[Resolution\|resolution]] 4.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7wtc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WTC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wtc OCA], [https://pdbe.org/7wtc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wtc RCSB], [https://www.ebi.ac.uk/pdbsum/7wtc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wtc ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/PYC_HUMAN PYC_HUMAN] Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:[https://omim.org/entry/266150 266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PYC_HUMAN PYC_HUMAN] Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pyruvate carboxylase (PC) catalyzes the two-step carboxylation of pyruvate to produce oxaloacetate, playing a key role in the maintenance of metabolic homeostasis in cells. Given its involvement in multiple diseases, PC has been regarded as a potential therapeutic target for obesity, diabetes, and cancer. Albeit acetyl-CoA has been recognized as the allosteric regulator of PC for over 60 years, the underlying mechanism of how acetyl-CoA induces PC activation remains enigmatic. Herein, by using time-resolved cryo-electron microscopy, we have captured the snapshots of PC transitional states during its catalytic cycle. These structures and the biochemical studies reveal that acetyl-CoA stabilizes PC in a catalytically competent conformation, which triggers a cascade of events, including ATP hydrolysis and the long-distance communication between the two reactive centers. These findings provide an integrated picture for PC catalysis and unveil the unique allosteric mechanism of acetyl-CoA in an essential biochemical reaction in all kingdoms of life.

-	~~Authors: Chai~~, P., Lan, P., Wu, J., Lei, M.	+	Mechanistic insight into allosteric activation of human pyruvate carboxylase by acetyl-CoA.,Chai P, Lan P, Li S, Yao D, Chang C, Cao M, Shen Y, Ge S, Wu J, Lei M, Fan X Mol Cell. 2022 Nov 3;82(21):4116-4130.e6. doi: 10.1016/j.molcel.2022.09.033. Epub, 2022 Oct 24. PMID:36283412<ref>PMID:36283412</ref>

-	~~Description: Cryo-EM structure~~ of ~~human pyruvate carboxylase with acetyl-CoA in~~ the ~~ground state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Wu, J~~]]	+	<div class="pdbe-citations 7wtc" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Lan,~~ P]]	+	== References ==
-	[[Category: ~~Chai,~~ P]]	+	<references/>
-	[[Category: Lei, M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chai P]]
		+	[[Category: Lan P]]
		+	[[Category: Lei M]]
		+	[[Category: Wu J]]

OCA at 05:12, 2 March 2022

OCA — Wed, 02 Mar 2022 05:12:51 GMT

←Older revision		Revision as of 05:12, 2 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wtc is ON HOLD	+	The entry 7wtc is ON HOLD until Paper Publication

	Authors: Chai, P., Lan, P., Wu, J., Lei, M.		Authors: Chai, P., Lan, P., Wu, J., Lei, M.
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	Description: Cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state		Description: Cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Wu, J]]
	[[Category: Lan, P]]		[[Category: Lan, P]]
	[[Category: Chai, P]]		[[Category: Chai, P]]
	[[Category: Lei, M]]		[[Category: Lei, M]]
-	[[Category: Wu, J]]

OCA at 09:18, 16 February 2022

OCA — Wed, 16 Feb 2022 09:18:45 GMT

←Older revision		Revision as of 09:18, 16 February 2022
Line 5:		Line 5:
	Authors: Chai, P., Lan, P., Wu, J., Lei, M.		Authors: Chai, P., Lan, P., Wu, J., Lei, M.

-	Description: ~~cryo~~-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state	+	Description: Cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wu, J]]
	[[Category: Lan, P]]		[[Category: Lan, P]]
	[[Category: Chai, P]]		[[Category: Chai, P]]
	[[Category: Lei, M]]		[[Category: Lei, M]]
		+	[[Category: Wu, J]]

OCA: Protected "7wtc" [edit=sysop:move=sysop]

OCA — Wed, 09 Feb 2022 06:29:49 GMT

Protected "7wtc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:29, 9 February 2022

OCA: New page: '''Unreleased structure''' The entry 7wtc is ON HOLD Authors: Chai, P., Lan, P., Wu, J., Lei, M. Description: cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the gro...

OCA — Wed, 09 Feb 2022 06:29:48 GMT

New page: '''Unreleased structure''' The entry 7wtc is ON HOLD Authors: Chai, P., Lan, P., Wu, J., Lei, M. Description: cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the gro...

New page

'''Unreleased structure'''

The entry 7wtc is ON HOLD

Authors: Chai, P., Lan, P., Wu, J., Lei, M.

Description: cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the ground state
[[Category: Unreleased Structures]]
[[Category: Wu, J]]
[[Category: Lan, P]]
[[Category: Chai, P]]
[[Category: Lei, M]]