OCA at 10:40, 6 April 2022

OCA — Wed, 06 Apr 2022 10:40:16 GMT

←Older revision		Revision as of 10:40, 6 April 2022
Line 1:		Line 1:

-	==~~Structural basis~~ of ~~dynamic~~ P5CS ~~filaments~~==	+	==GPR domain closed form of Drosophila P5CS filament with glutamate, ATP, and NADPH==
-	<StructureSection load='7wxg' size='340' side='right'caption='[[7wxg]]' scene=''>	+	<StructureSection load='7wxg' size='340' side='right'caption='[[7wxg]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wxg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WXG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wxg OCA], [https://pdbe.org/7wxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wxg RCSB], [https://www.ebi.ac.uk/pdbsum/7wxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wxg ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wxg OCA], [https://pdbe.org/7wxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wxg RCSB], [https://www.ebi.ac.uk/pdbsum/7wxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wxg ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bifunctional enzyme Delta(1)-pyrroline-5-carboxylate synthase (P5CS) is vital to the synthesis of proline and ornithine, playing an essential role in human health and agriculture. Pathogenic mutations in the P5CS gene (ALDH18A1) lead to neurocutaneous syndrome and skin relaxation connective tissue disease in humans, and P5CS deficiency seriously damages the ability to resist adversity in plants. We have recently found that P5CS forms cytoophidia in vivo and filaments in vitro. However, it is difficult to appreciate the function of P5CS filamentation without precise structures. Using cryo-electron microscopy, here we solve the structures of Drosophila full-length P5CS in three states at resolution from 3.1 to 4.3 A. We observe distinct ligand-binding states and conformational changes for the GK and GPR domains, respectively. Divergent helical filaments are assembled by P5CS tetramers and stabilized by multiple interfaces. Point mutations disturbing those interfaces prevent P5CS filamentation and greatly reduce the enzymatic activity. Our findings reveal that filamentation is crucial for the coordination between the GK and GPR domains, providing a structural basis for the catalytic function of P5CS filaments.
		+
		+	Structural basis of dynamic P5CS filaments.,Zhong J, Guo CJ, Zhou X, Chang CC, Yin B, Zhang T, Hu HH, Lu GM, Liu JL Elife. 2022 Mar 14;11. pii: 76107. doi: 10.7554/eLife.76107. PMID:35286254<ref>PMID:35286254</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wxg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guo CJ]]	+	[[Category: Guo, C J]]
-	[[Category: Liu JL]]	+	[[Category: Liu, J L]]
-	[[Category: Zhong J]]	+	[[Category: Zhong, J]]
-	[[Category: Zhou X]]	+	[[Category: Zhou, X]]
		+	[[Category: Aldh18a1]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Delta-1-pyrroline-5-carboxylate synthase]]
		+	[[Category: Filament]]
		+	[[Category: Glutamate-bound]]
		+	[[Category: Transferase]]

OCA at 10:49, 30 March 2022

OCA — Wed, 30 Mar 2022 10:49:47 GMT

←Older revision		Revision as of 10:49, 30 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wxg is ~~ON HOLD~~	+	==Structural basis of dynamic P5CS filaments==
-		+	<StructureSection load='7wxg' size='340' side='right'caption='[[7wxg]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WXG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wxg OCA], [https://pdbe.org/7wxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wxg RCSB], [https://www.ebi.ac.uk/pdbsum/7wxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wxg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Guo CJ]]
		+	[[Category: Liu JL]]
		+	[[Category: Zhong J]]
		+	[[Category: Zhou X]]

OCA: Protected "7wxg" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2022 06:41:22 GMT

Protected "7wxg" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:41, 23 February 2022

OCA: New page: '''Unreleased structure''' The entry 7wxg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 23 Feb 2022 06:41:21 GMT

New page: '''Unreleased structure''' The entry 7wxg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7wxg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7wxg - Revision history

OCA at 10:40, 6 April 2022

OCA at 10:49, 30 March 2022

OCA: Protected "7wxg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7wxg is ON HOLD Authors: Description: Category: Unreleased Structures