7wzu - Revision history

OCA at 17:46, 29 November 2023

OCA — Wed, 29 Nov 2023 17:46:31 GMT

←Older revision		Revision as of 17:46, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7wzu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[7wzu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WZU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.954Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wzu OCA], [https://pdbe.org/7wzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wzu RCSB], [https://www.ebi.ac.uk/pdbsum/7wzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wzu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wzu OCA], [https://pdbe.org/7wzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wzu RCSB], [https://www.ebi.ac.uk/pdbsum/7wzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wzu ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:54, 25 January 2023

OCA — Wed, 25 Jan 2023 06:54:57 GMT

←Older revision		Revision as of 06:54, 25 January 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7wzu is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of metallo-beta-lactamase IMP-6.==
		+	<StructureSection load='7wzu' size='340' side='right'caption='[[7wzu]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7wzu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WZU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wzu OCA], [https://pdbe.org/7wzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wzu RCSB], [https://www.ebi.ac.uk/pdbsum/7wzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wzu ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K4PWX3_ECOLX K4PWX3_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-negative bacteria producing metallo-beta-lactamases (MBLs) have become a considerable threat to public health. MBLs including the IMP, VIM, and NDM types are Zn(II) enzymes that hydrolyze the beta-lactam ring present in a broad range of antibiotics, such as N-benzylpenicillin, meropenem, and imipenem. Among IMPs, IMP-1 and IMP-6 differ in a single amino acid substitution at position 262, where serine in IMP-1 is replaced by glycine in IMP-6, conferring a change in substrate specificity. To investigate how this mutation influences enzyme function, we examined lactamase inhibition by thiol compounds. Ethyl 3-mercaptopropionate acted as a competitive inhibitor of IMP-1, but a noncompetitive inhibitor of IMP-6. A comparison of the crystal structures previously reported for IMP-1 (PDB code: 5EV6) and IMP-6 (PDB code: 6LVJ) revealed a hydrogen bond between the side chain of Ser262 and Cys221 in IMP-1 but the absence of hydrogen bond in IMP-6, which affects the Zn2 coordination sphere in its active site. We investigated the demetallation rates of IMP-1 and IMP-6 in the presence of chelating agent ethylenediaminetetraacetic acid (EDTA) and found that the demetallation reactions had fast and slow phases with a first-order rate constant (k(fast) = 1.76 h(-1), k(slow) = 0.108 h(-1) for IMP-1, and k(fast) = 14.0 h(-1) and k(slow) = 1.66 h(-1) for IMP-6). The difference in the flexibility of the Zn2 coordination sphere between IMP-1 and IMP-6 may influence the demetallation rate, the catalytic efficiency against beta-lactam antibiotics, and the inhibitory effect of thiol compounds.

-	~~Authors~~:	+	Difference in the Inhibitory Effect of Thiol Compounds and Demetallation Rates from the Zn(II) Active Site of Metallo-beta-lactamases (IMP-1 and IMP-6) Associated with a Single Amino Acid Substitution.,Yamaguchi Y, Kato K, Ichimaru Y, Uenosono Y, Tawara S, Ito R, Matsuse N, Wachino JI, Toma-Fukai S, Jin W, Arakawa Y, Otsuka M, Fujita M, Fukuishi N, Sugiura K, Imai M, Kurosaki H ACS Infect Dis. 2023 Jan 13;9(1):65-78. doi: 10.1021/acsinfecdis.2c00395. Epub , 2022 Dec 15. PMID:36519431<ref>PMID:36519431</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7wzu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Kurosaki H]]
		+	[[Category: Yamaguchi Y]]

OCA at 09:10, 6 April 2022

OCA — Wed, 06 Apr 2022 09:10:44 GMT

←Older revision		Revision as of 09:10, 6 April 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wzu is ON HOLD	+	The entry 7wzu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 05:03, 9 March 2022

OCA — Wed, 09 Mar 2022 05:03:05 GMT

←Older revision		Revision as of 05:03, 9 March 2022
Line 3:		Line 3:
	The entry 7wzu is ON HOLD		The entry 7wzu is ON HOLD

-	Authors: ~~Yamaguchi, Y., Kurosaki, H.~~	+	Authors:

-	Description: ~~Crystal structure of metallo-beta-lactamase IMP-6.~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Kurosaki, H]]
-	[[Category: Yamaguchi, Y]]

OCA: Protected "7wzu" [edit=sysop:move=sysop]

OCA — Wed, 02 Mar 2022 05:14:18 GMT

Protected "7wzu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7wzu is ON HOLD Authors: Yamaguchi, Y., Kurosaki, H. Description: Crystal structure of metallo-beta-lactamase IMP-6. [[Category: Unreleased Structu...

OCA — Wed, 02 Mar 2022 05:14:17 GMT

New page: '''Unreleased structure''' The entry 7wzu is ON HOLD Authors: Yamaguchi, Y., Kurosaki, H. Description: Crystal structure of metallo-beta-lactamase IMP-6. [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 7wzu is ON HOLD

Authors: Yamaguchi, Y., Kurosaki, H.

Description: Crystal structure of metallo-beta-lactamase IMP-6.
[[Category: Unreleased Structures]]
[[Category: Kurosaki, H]]
[[Category: Yamaguchi, Y]]