OCA at 07:26, 9 November 2022

OCA — Wed, 09 Nov 2022 07:26:29 GMT

←Older revision		Revision as of 07:26, 9 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7x26 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==S41 neutralizing antibody Fab(MERS-CoV)==
		+	<StructureSection load='7x26' size='340' side='right'caption='[[7x26]], [[Resolution\|resolution]] 3.69Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7x26]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X26 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x26 OCA], [https://pdbe.org/7x26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x26 RCSB], [https://www.ebi.ac.uk/pdbsum/7x26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x26 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A168DJZ9_MERS A0A168DJZ9_MERS] Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.[HAMAP-Rule:MF_04099] Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neutralizing monoclonal antibodies (mAbs) against highly pathogenic coronaviruses represent promising candidates for clinical intervention. Here, we isolated a potent neutralizing monoclonal antibody, MERS-S41, from a yeast displayed scFv library using the S protein as a bait. To uncover the neutralization mechanism, we determined structures of MERS-S41 Fab in complex with the trimeric spike glycoprotein by cryoelectron microscopy (cryo-EM). We observed four distinct classes of the complex structure, which showed that the MERS-S41 Fab bound to the "up" receptor binding domain (RBD) with full saturation and also bound to an accessible partially lifted "down" RBD, providing a structural basis for understanding how mAbs bind to trimeric spike glycoproteins. Structure analysis of the epitope and cell surface staining assays demonstrated that virus entry is blocked predominantly by direct competition with the host receptor, dipeptidyl peptidase-4 (DPP4).

-	~~Authors~~:	+	Cryoelectron microscopy structures of a human neutralizing antibody bound to MERS-CoV spike glycoprotein.,Zhang S, Jia W, Zeng J, Li M, Wang Z, Zhou H, Zhang L, Wang X Front Microbiol. 2022 Sep 28;13:988298. doi: 10.3389/fmicb.2022.988298., eCollection 2022. PMID:36246239<ref>PMID:36246239</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7x26" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Wang XW]]
		+	[[Category: Zeng JW]]
		+	[[Category: Zhang SY]]

OCA at 09:11, 6 April 2022

OCA — Wed, 06 Apr 2022 09:11:44 GMT

←Older revision		Revision as of 09:11, 6 April 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7x26 is ON HOLD	+	The entry 7x26 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7x26" [edit=sysop:move=sysop]

OCA — Wed, 02 Mar 2022 05:18:03 GMT

Protected "7x26" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7x26 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Mar 2022 05:17:59 GMT

New page: '''Unreleased structure''' The entry 7x26 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7x26 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7x26 - Revision history

OCA at 07:26, 9 November 2022

OCA at 09:11, 6 April 2022

OCA: Protected "7x26" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7x26 is ON HOLD Authors: Description: Category: Unreleased Structures