7x8b - Revision history

OCA at 17:49, 29 November 2023

OCA — Wed, 29 Nov 2023 17:49:13 GMT

←Older revision		Revision as of 17:49, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7x8b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8B FirstGlance]. <br>		<table><tr><td colspan='2'>[[7x8b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8b OCA], [https://pdbe.org/7x8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8b RCSB], [https://www.ebi.ac.uk/pdbsum/7x8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8b OCA], [https://pdbe.org/7x8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8b RCSB], [https://www.ebi.ac.uk/pdbsum/7x8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8b ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:20, 18 January 2023

OCA — Wed, 18 Jan 2023 07:20:06 GMT

←Older revision		Revision as of 07:20, 18 January 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7x8b is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of ENL T1 mutant YEATS domain in complex with histone H3 acetylation at K27==
		+	<StructureSection load='7x8b' size='340' side='right'caption='[[7x8b]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7x8b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8B FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8b OCA], [https://pdbe.org/7x8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8b RCSB], [https://www.ebi.ac.uk/pdbsum/7x8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8b ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN] A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with KMT2A/MLL1. The result is a rogue activator protein.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN] Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.<ref>PMID:20159561</ref> <ref>PMID:20471948</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Growing evidence suggests prevalence of transcriptional condensates on chromatin, yet their mechanisms of formation and functional significance remain largely unclear. In human cancer, a series of mutations in the histone acetylation reader ENL create gain-of-function mutants with increased transcriptional activation ability. Here, we show that these mutations, clustered in ENL's structured acetyl-reading YEATS domain, trigger aberrant condensates at native genomic targets through multivalent homotypic and heterotypic interactions. Mechanistically, mutation-induced structural changes in the YEATS domain, ENL's two disordered regions of opposing charges, and the incorporation of extrinsic elongation factors are all required for ENL condensate formation. Extensive mutagenesis establishes condensate formation as a driver of oncogenic gene activation. Furthermore, expression of ENL mutants beyond the endogenous level leads to non-functional condensates. Our findings provide new mechanistic and functional insights into cancer-associated condensates and support condensate dysregulation as an oncogenic mechanism.

-	~~Authors:~~ Li, Y., ~~Peng~~, B., Li, H.	+	Hotspot mutations in the structured ENL YEATS domain link aberrant transcriptional condensates and cancer.,Song L, Yao X, Li H, Peng B, Boka AP, Liu Y, Chen G, Liu Z, Mathias KM, Xia L, Li Q, Mir M, Li Y, Li H, Wan L Mol Cell. 2022 Nov 3;82(21):4080-4098.e12. doi: 10.1016/j.molcel.2022.09.034. , Epub 2022 Oct 21. PMID:36272410<ref>PMID:36272410</ref>

-	~~Description: Crystal structure~~ of ~~ENL T1 mutant YEATS domain in complex with histone H3 acetylation at K27~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Peng, B~~]]	+	<div class="pdbe-citations 7x8b" style="background-color:#fffaf0;"></div>
-	[[Category: Li, H]]	+	== References ==
-	[[Category: Li, Y]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Li H]]
		+	[[Category: Li Y]]
		+	[[Category: Peng B]]

OCA: Protected "7x8b" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2022 10:00:05 GMT

Protected "7x8b" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:00, 23 March 2022

OCA: New page: '''Unreleased structure''' The entry 7x8b is ON HOLD until Paper Publication Authors: Li, Y., Peng, B., Li, H. Description: Crystal structure of ENL T1 mutant YEATS domain in complex w...

OCA — Wed, 23 Mar 2022 09:59:59 GMT

New page: '''Unreleased structure''' The entry 7x8b is ON HOLD until Paper Publication Authors: Li, Y., Peng, B., Li, H. Description: Crystal structure of ENL T1 mutant YEATS domain in complex w...

New page

'''Unreleased structure'''

The entry 7x8b is ON HOLD until Paper Publication

Authors: Li, Y., Peng, B., Li, H.

Description: Crystal structure of ENL T1 mutant YEATS domain in complex with histone H3 acetylation at K27
[[Category: Unreleased Structures]]
[[Category: Peng, B]]
[[Category: Li, H]]
[[Category: Li, Y]]