OCA at 17:49, 29 November 2023

OCA — Wed, 29 Nov 2023 17:49:15 GMT

←Older revision		Revision as of 17:49, 29 November 2023
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	==Crystal structure of a KTSC family protein from Euryarchaeon Methanolobus vulcani==		==Crystal structure of a KTSC family protein from Euryarchaeon Methanolobus vulcani==
-	<StructureSection load='7x8c' size='340' side='right'caption='[[7x8c]]' scene=''>	+	<StructureSection load='7x8c' size='340' side='right'caption='[[7x8c]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7x8c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanolobus_vulcani Methanolobus vulcani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8C FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8c OCA], [https://pdbe.org/7x8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8c RCSB], [https://www.ebi.ac.uk/pdbsum/7x8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8c ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.73Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8c OCA], [https://pdbe.org/7x8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8c RCSB], [https://www.ebi.ac.uk/pdbsum/7x8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A7Z7AYG0_9EURY A0A7Z7AYG0_9EURY]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lysine (K) tRNA synthetase C-terminal (KTSC) domain containing proteins are widely spread in Bacteria, Archaea and Viruses, but the function of this short domain is unclear. The occurrence of the fusion of KTSC domain to a catalytic domain or domains related to DNA or RNA metabolisms suggests its potential role in DNA or RNA binding. Here, we report the characterization of Mvu8s from Methanolobus vulcani, which consists of a single KTSC domain. Mvu8s binds specifically to ssDNA with an affinity approximately 40- and 10-fold higher than those for dsDNA and ssRNA in vitro, respectively. It shows a slight preference to the G-rich DNA sequence but barely binds the A-stretch. Crystal structure of Mvu8s shows that it forms a homo-tetramer, with each monomer composed of a four-strand antiparallel beta-sheet and a helix-turn-helix in the order of beta1-beta2-beta3-alpha1-alpha2-beta4. Four basic residues (R3, R7, K54 and K58) were found to serve important roles in ssDNA-binding. And, the spiral arrangement of the DNA interfaces in Mvu8s homo-tetramer presumably results in ssDNA wrapping. Our results not only offer clues of the functions of the KTSC domain containing proteins but also expand our knowledge on the non-oligonucleotide-binding (OB) fold single-stranded DNA-binding proteins in Archaea.
		+
		+	Biochemical and structural characterization of a KTSC family single-stranded DNA-binding protein from Euryarchaea.,Tian L, Zhu K, Chen Y, Zheng X, Zhang H, Geng Z, Li W, Ding N, Chen J, Dong Y, Cao P, Gong Y, Zhang Z Int J Biol Macromol. 2022 Sep 1;216:618-628. doi: 10.1016/j.ijbiomac.2022.07.015., Epub 2022 Jul 7. PMID:35809674<ref>PMID:35809674</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7x8c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Methanolobus vulcani]]
	[[Category: Cao P]]		[[Category: Cao P]]
	[[Category: Chen YY]]		[[Category: Chen YY]]

OCA at 06:23, 18 August 2022

OCA — Thu, 18 Aug 2022 06:23:38 GMT

←Older revision		Revision as of 06:23, 18 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7x8c is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of a KTSC family protein from Euryarchaeon Methanolobus vulcani==
-		+	<StructureSection load='7x8c' size='340' side='right'caption='[[7x8c]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X8C FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x8c OCA], [https://pdbe.org/7x8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x8c RCSB], [https://www.ebi.ac.uk/pdbsum/7x8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x8c ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cao P]]
		+	[[Category: Chen YY]]
		+	[[Category: Gong Y]]
		+	[[Category: Zhang ZF]]
		+	[[Category: Zhu KL]]

OCA at 01:40, 21 April 2022

OCA — Thu, 21 Apr 2022 01:40:41 GMT

←Older revision		Revision as of 01:40, 21 April 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7x8c is ON HOLD	+	The entry 7x8c is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7x8c" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2022 10:00:10 GMT

Protected "7x8c" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:00, 23 March 2022

OCA: New page: '''Unreleased structure''' The entry 7x8c is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 23 Mar 2022 10:00:08 GMT

New page: '''Unreleased structure''' The entry 7x8c is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7x8c is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7x8c - Revision history

OCA at 17:49, 29 November 2023

OCA at 06:23, 18 August 2022

OCA at 01:40, 21 April 2022

OCA: Protected "7x8c" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7x8c is ON HOLD Authors: Description: Category: Unreleased Structures