OCA at 05:20, 9 August 2023

OCA — Wed, 09 Aug 2023 05:20:49 GMT

←Older revision		Revision as of 05:20, 9 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7xfz is ~~ON HOLD until 2024~~-10-04	+	==CryoEM structure of type IV-A Csf-crRNAsp14-dsDNA ternary complex==
		+	<StructureSection load='7xfz' size='340' side='right'caption='[[7xfz]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xfz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XFZ FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=23G:GUANOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>23G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xfz OCA], [https://pdbe.org/7xfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xfz RCSB], [https://www.ebi.ac.uk/pdbsum/7xfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xfz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types for which the mechanistic details of their function remain unknown. Here, we provide high-resolution functional snapshots of type IV-A Csf complexes before and after target dsDNA binding, either in the absence or presence of CasDinG, revealing the mechanisms underlying Csf(crRNA) complex assembly, "DWN" PAM-dependent dsDNA targeting, R-loop formation, and CasDinG recruitment. Furthermore, we establish that CasDinG, a signature DinG family helicase, harbors ssDNA-stimulated ATPase activity and ATP-dependent 5'-3' DNA helicase activity. In addition, we show that CasDinG unwinds the non-target strand (NTS) and target strand (TS) of target dsDNA from the Csf(crRNA) complex. These molecular details advance our mechanistic understanding of type IV-A CRISPR-Csf function and should enable Csf complexes to be harnessed as genome-engineering tools for biotechnological applications.

-	~~Authors~~:	+	Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment.,Cui N, Zhang JT, Liu Y, Liu Y, Liu XY, Wang C, Huang H, Jia N Mol Cell. 2023 Jul 20;83(14):2493-2508.e5. doi: 10.1016/j.molcel.2023.05.036. , Epub 2023 Jun 20. PMID:37343553<ref>PMID:37343553</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7xfz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Cui N]]
		+	[[Category: Huang HD]]
		+	[[Category: Jia N]]
		+	[[Category: Zhang JT]]

OCA at 04:51, 8 February 2023

OCA — Wed, 08 Feb 2023 04:51:03 GMT

←Older revision		Revision as of 04:51, 8 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xfz is ON HOLD until ~~Paper Publication~~	+	The entry 7xfz is ON HOLD until 2024-10-04

	Authors:		Authors:

OCA at 11:39, 4 May 2022

OCA — Wed, 04 May 2022 11:39:12 GMT

←Older revision		Revision as of 11:39, 4 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xfz is ON HOLD	+	The entry 7xfz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7xfz" [edit=sysop:move=sysop]

OCA — Wed, 13 Apr 2022 08:42:06 GMT

Protected "7xfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:42, 13 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xfz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Apr 2022 08:42:05 GMT

New page: '''Unreleased structure''' The entry 7xfz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7xfz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7xfz - Revision history

OCA at 05:20, 9 August 2023

OCA at 04:51, 8 February 2023

OCA at 11:39, 4 May 2022

OCA: Protected "7xfz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7xfz is ON HOLD Authors: Description: Category: Unreleased Structures