7xh9 - Revision history

OCA at 17:53, 29 November 2023

OCA — Wed, 29 Nov 2023 17:53:51 GMT

←Older revision		Revision as of 17:53, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7xh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XH9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7xh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XH9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xh9 OCA], [https://pdbe.org/7xh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xh9 RCSB], [https://www.ebi.ac.uk/pdbsum/7xh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xh9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xh9 OCA], [https://pdbe.org/7xh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xh9 RCSB], [https://www.ebi.ac.uk/pdbsum/7xh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xh9 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:24, 15 February 2023

OCA — Wed, 15 Feb 2023 10:24:20 GMT

←Older revision		Revision as of 10:24, 15 February 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7xh9 is ~~ON HOLD~~	+	==Crystal structure of a dimeric interlocked parallel G-quadruplex==
		+	<StructureSection load='7xh9' size='340' side='right'caption='[[7xh9]], [[Resolution\|resolution]] 1.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XH9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xh9 OCA], [https://pdbe.org/7xh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xh9 RCSB], [https://www.ebi.ac.uk/pdbsum/7xh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xh9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K(+) solution. Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures of 93del and its variants. While confirming the overall dimeric interlocked G-quadruplex folding topology previously determined by NMR, our results reveal important detailed structural information, particularly the formation of a water-mediated AGGGG pentad. These insights allow us to better understand the formation of various structural elements in G-quadruplexes and should be useful for designing and manipulating G-quadruplex scaffolds with desired properties.

-	~~Authors~~: ~~Ngo, K.H~~., Liew~~, C.W.~~, Lattmann, S., Winnerdy, F.R., ~~Phan, A~~.T.	+	Crystal structures of an HIV-1 integrase aptamer: Formation of a water-mediated AGGGG pentad in an interlocked G-quadruplex.,Ngo KH, Liew CW, Lattmann S, Winnerdy FR, Phan AT Biochem Biophys Res Commun. 2022 Jul 12;613:153-158. doi: , 10.1016/j.bbrc.2022.04.020. Epub 2022 Apr 10. PMID:35561583<ref>PMID:35561583</ref>

-	~~Description: Crystal structure of~~ a ~~dimeric interlocked parallel G~~-~~quadruplex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Lattmann, S]]	+	<div class="pdbe-citations 7xh9" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Phan, A.T~~]]	+	== References ==
-	[[Category: ~~Winnerdy, F.R~~]]	+	<references/>
-	[[Category: ~~Ngo, K.H~~]]	+	__TOC__
-	[[Category: ~~Liew, C.W~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Lattmann S]]
		+	[[Category: Liew CW]]
		+	[[Category: Ngo KH]]
		+	[[Category: Phan AT]]
		+	[[Category: Winnerdy FR]]

OCA: Protected "7xh9" [edit=sysop:move=sysop]

OCA — Wed, 13 Apr 2022 08:44:09 GMT

Protected "7xh9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:44, 13 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xh9 is ON HOLD Authors: Ngo, K.H., Liew, C.W., Lattmann, S., Winnerdy, F.R., Phan, A.T. Description: Crystal structure of a dimeric interlocked pa...

OCA — Wed, 13 Apr 2022 08:44:08 GMT

New page: '''Unreleased structure''' The entry 7xh9 is ON HOLD Authors: Ngo, K.H., Liew, C.W., Lattmann, S., Winnerdy, F.R., Phan, A.T. Description: Crystal structure of a dimeric interlocked pa...

New page

'''Unreleased structure'''

The entry 7xh9 is ON HOLD

Authors: Ngo, K.H., Liew, C.W., Lattmann, S., Winnerdy, F.R., Phan, A.T.

Description: Crystal structure of a dimeric interlocked parallel G-quadruplex
[[Category: Unreleased Structures]]
[[Category: Lattmann, S]]
[[Category: Phan, A.T]]
[[Category: Winnerdy, F.R]]
[[Category: Ngo, K.H]]
[[Category: Liew, C.W]]