7xhx - Revision history

OCA at 17:54, 29 November 2023

OCA — Wed, 29 Nov 2023 17:54:11 GMT

←Older revision		Revision as of 17:54, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7xhx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6lvj 6lvj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHX FirstGlance]. <br>		<table><tr><td colspan='2'>[[7xhx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6lvj 6lvj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhx OCA], [https://pdbe.org/7xhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhx RCSB], [https://www.ebi.ac.uk/pdbsum/7xhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhx OCA], [https://pdbe.org/7xhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhx RCSB], [https://www.ebi.ac.uk/pdbsum/7xhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhx ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 7xhx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7xhx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:17, 15 February 2023

OCA — Wed, 15 Feb 2023 10:17:15 GMT

←Older revision		Revision as of 10:17, 15 February 2023
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-	'''Unreleased structure'''

-	~~The~~ entry 7xhx is ~~ON HOLD until 2024~~-04-11	+	==Crystal structure of metallo-beta-lactamase IMP-6==
		+	<StructureSection load='7xhx' size='340' side='right'caption='[[7xhx]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xhx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6lvj 6lvj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhx OCA], [https://pdbe.org/7xhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhx RCSB], [https://www.ebi.ac.uk/pdbsum/7xhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K4PWX3_ECOLX K4PWX3_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	IMP-type metallo-beta-lactamases confer resistance to carbapenems and a broad spectrum of beta-lactam antibiotics. IMP-6 and IMP-1 differ by only a point mutation: Ser262 in IMP-1 and Gly262 in IMP-6. The kcat/Km values of IMP-1 for imipenem and meropenem are nearly identical; however, for IMP-6, the kcat/Km for meropenem is 7-fold that for imipenem. In clinical practice, this may result in an ineffective therapeutic regimen and, consequently, in treatment failure. Here, we report the crystal structures of IMP-6 and IMP-1 with the same space group and similar cell constants at resolutions of 1.70 and 1.94 A, respectively. The overall structures of IMP-6 and IMP-1 are similar. However, the loop region (residues 60-66), which participates in substrate binding, is more flexible in IMP-6 than in IMP-1. This difference in flexibility determines the substrate specificity of IMP-type metallo-beta-lactamases for imipenem and meropenem. The amino acid at position 262 alters the mobility of His263; this affects the flexibility of the loop via a hydrogen bond with Pro68, which plays the role of a hinge in IMP-type metallo-beta-lactamases. The substitution of Pro68 with a glycine elicited an increase in the Km of IMP-6 for imipenem, whereas the affinity for meropenem remained unchanged.

-	~~Authors: Yamamoto~~, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.	+	Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-beta-lactamases.,Yamamoto K, Tanaka H, Kurisu G, Nakano R, Yano H, Sakai H J Biochem. 2022 Dec 27;173(1):21-30. doi: 10.1093/jb/mvac080. PMID:36174533<ref>PMID:36174533</ref>

-	~~Description: Crystal structure~~ of ~~metallo~~-~~beta~~-~~lactamase IMP-6~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Yamamoto, K~~]]	+	<div class="pdbe-citations 7xhx" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Tanaka, H~~]]	+	== References ==
-	[[Category: ~~Kurisu, G~~]]	+	<references/>
-	[[Category: Sakai, H]]	+	__TOC__
-	[[Category: ~~Nakano, R~~]]	+	</StructureSection>
-	[[Category: Yano, H]]	+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Kurisu G]]
		+	[[Category: Nakano R]]
		+	[[Category: Sakai H]]
		+	[[Category: Tanaka H]]
		+	[[Category: Yamamoto K]]
		+	[[Category: Yano H]]

OCA at 06:58, 22 June 2022

OCA — Wed, 22 Jun 2022 06:58:31 GMT

←Older revision		Revision as of 06:58, 22 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xhx is ON HOLD until ~~Apr 11~~ 2024	+	The entry 7xhx is ON HOLD until 2024-04-11

	Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.		Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.
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	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]
	[[Category: Sakai, H]]		[[Category: Sakai, H]]
-	[[Category: Yano, H]]
	[[Category: Nakano, R]]		[[Category: Nakano, R]]
		+	[[Category: Yano, H]]

OCA at 03:56, 8 June 2022

OCA — Wed, 08 Jun 2022 03:56:08 GMT

←Older revision		Revision as of 03:56, 8 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xhx is ON HOLD	+	The entry 7xhx is ON HOLD until Apr 11 2024

	Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.		Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.

OCA: Protected "7xhx" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2022 01:42:52 GMT

Protected "7xhx" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:42, 21 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xhx is ON HOLD Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H. Description: Crystal structure of metallo-beta-lacta...

OCA — Thu, 21 Apr 2022 01:42:52 GMT

New page: '''Unreleased structure''' The entry 7xhx is ON HOLD Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H. Description: Crystal structure of metallo-beta-lacta...

New page

'''Unreleased structure'''

The entry 7xhx is ON HOLD

Authors: Yamamoto, K., Tanaka, H., Kurisu, G., Nakano, R., Yano, H., Sakai, H.

Description: Crystal structure of metallo-beta-lactamase IMP-6
[[Category: Unreleased Structures]]
[[Category: Yamamoto, K]]
[[Category: Tanaka, H]]
[[Category: Kurisu, G]]
[[Category: Sakai, H]]
[[Category: Yano, H]]
[[Category: Nakano, R]]