OCA at 07:28, 8 March 2023

OCA — Wed, 08 Mar 2023 07:28:27 GMT

←Older revision		Revision as of 07:28, 8 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7xkz is ~~ON HOLD until Paper~~ Publication	+	==Solution structure of subunit epsilon of the Mycobacterium abscessus F-ATP synthase==
		+	<StructureSection load='7xkz' size='340' side='right'caption='[[7xkz]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xkz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacteroides_abscessus Mycobacteroides abscessus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XKZ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xkz OCA], [https://pdbe.org/7xkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xkz RCSB], [https://www.ebi.ac.uk/pdbsum/7xkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xkz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0U0YP27_9MYCO A0A0U0YP27_9MYCO] Produces ATP from ADP in the presence of a proton gradient across the membrane.[HAMAP-Rule:MF_00530]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium abscessus (Mab) is a nontuberculous mycobacterium of increasing clinical relevance. The rapidly growing opportunistic pathogen is intrinsically multi-drug-resistant and causes difficult-to-cure lung disease. Adenosine triphosphate, generated by the essential F(1) F(O) ATP synthase, is the major energy currency of the pathogen, bringing this enzyme complex into focus for the discovery of novel antimycobacterial compounds. Coupling of proton translocation through the membrane-embedded F(O) sector and ATP formation in the F(1) headpiece of the bipartite F(1) F(O) ATP synthase occurs via the central stalk subunits gamma and epsilon. Here, we used solution NMR spectroscopy to resolve the first atomic structure of the Mab subunit epsilon (Mabepsilon), showing that it consists of an N-terminal beta-barrel domain (NTD) and a helix-loop-helix motif in its C-terminal domain (CTD). NMR relaxation measurements of Mabepsilon shed light on dynamic epitopes and amino acids relevant for coupling processes within the protein. We describe structural differences between other mycobacterial epsilon subunits and Mabepsilon's lack of ATP binding. Based on the structural insights, we conducted an in silico inhibitor screen. One hit, Ep1MabF1, was shown to inhibit the growth of Mab and bacterial ATP synthesis. NMR titration experiments and docking studies described the binding epitopes of Ep1MabF1 on Mabepsilon. Together, our data demonstrate the potential to develop inhibitors targeting the epsilon subunit of Mab F(1) F(O) ATP synthase to interrupt the coupling process.

-	~~Authors:~~ Shin, J~~., Grueber, G.~~, Harikishore, A., Wong, ~~C.F., Prya, R.~~, Dick, T.	+	Atomic solution structure of Mycobacterium abscessus F-ATP synthase subunit epsilon and identification of Ep1MabF1 as a targeted inhibitor.,Shin J, Harikishore A, Wong CF, Ragunathan P, Dick T, Gruber G FEBS J. 2022 Oct;289(20):6308-6323. doi: 10.1111/febs.16536. Epub 2022 Jun 5. PMID:35612822<ref>PMID:35612822</ref>

-	~~Description: Solution structure of subunit epsilon~~ of the ~~Mycobacterium abscessus F~~-~~ATP synthase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Prya, R~~]]	+	<div class="pdbe-citations 7xkz" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Shin, J~~]]	+	== References ==
-	[[Category: Grueber, G]]	+	<references/>
-	[[Category: Harikishore, A]]	+	__TOC__
-	[[Category: ~~Dick, T~~]]	+	</StructureSection>
-	[[Category: Wong~~, C.F~~]]	+	[[Category: Large Structures]]
		+	[[Category: Mycobacteroides abscessus]]
		+	[[Category: Dick T]]
		+	[[Category: Grueber G]]
		+	[[Category: Harikishore A]]
		+	[[Category: Prya R]]
		+	[[Category: Shin J]]
		+	[[Category: Wong CF]]

OCA at 03:54, 15 June 2022

OCA — Wed, 15 Jun 2022 03:54:05 GMT

←Older revision		Revision as of 03:54, 15 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xkz is ON HOLD	+	The entry 7xkz is ON HOLD until Paper Publication

	Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T.		Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T.
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	Description: Solution structure of subunit epsilon of the Mycobacterium abscessus F-ATP synthase		Description: Solution structure of subunit epsilon of the Mycobacterium abscessus F-ATP synthase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Harikishore, A]]
-	[[Category: Dick, T]]
-	[[Category: Wong, C.F]]
	[[Category: Prya, R]]		[[Category: Prya, R]]
	[[Category: Shin, J]]		[[Category: Shin, J]]
	[[Category: Grueber, G]]		[[Category: Grueber, G]]
		+	[[Category: Harikishore, A]]
		+	[[Category: Dick, T]]
		+	[[Category: Wong, C.F]]

OCA: Protected "7xkz" [edit=sysop:move=sysop]

OCA — Wed, 27 Apr 2022 10:35:47 GMT

Protected "7xkz" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:35, 27 April 2022

OCA: New page: '''Unreleased structure''' The entry 7xkz is ON HOLD Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T. Description: Solution structure of subunit epsilon ...

OCA — Wed, 27 Apr 2022 10:35:46 GMT

New page: '''Unreleased structure''' The entry 7xkz is ON HOLD Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T. Description: Solution structure of subunit epsilon ...

New page

'''Unreleased structure'''

The entry 7xkz is ON HOLD

Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T.

Description: Solution structure of subunit epsilon of the Mycobacterium abscessus F-ATP synthase
[[Category: Unreleased Structures]]
[[Category: Harikishore, A]]
[[Category: Dick, T]]
[[Category: Wong, C.F]]
[[Category: Prya, R]]
[[Category: Shin, J]]
[[Category: Grueber, G]]

7xkz - Revision history

OCA at 07:28, 8 March 2023

OCA at 03:54, 15 June 2022

OCA: Protected "7xkz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7xkz is ON HOLD Authors: Shin, J., Grueber, G., Harikishore, A., Wong, C.F., Prya, R., Dick, T. Description: Solution structure of subunit epsilon ...