OCA at 05:33, 31 May 2023

OCA — Wed, 31 May 2023 05:33:55 GMT

←Older revision		Revision as of 05:33, 31 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7xmw is ~~ON HOLD~~	+	==Crystal structure of anti-CRISPR protein AcrVIA2==
		+	<StructureSection load='7xmw' size='340' side='right'caption='[[7xmw]], [[Resolution\|resolution]] 2.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xmw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptotrichia_wadei_F0279 Leptotrichia wadei F0279]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XMW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=SE:SELENIUM+ATOM'>SE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xmw OCA], [https://pdbe.org/7xmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xmw RCSB], [https://www.ebi.ac.uk/pdbsum/7xmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xmw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U2Q5N5_LEPWF U2Q5N5_LEPWF]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phages and non-phage derived bacteria have evolved many anti-CRISPR proteins (Acrs) to escape the adaptive immune system of prokaryotes. Thus Acrs can be applied as a regulatory tool for gene edition by CRISPR system. Recently, a non-phage derived AcrVIA2 has been identified as an inhibitor that blocks the editing activity of Cas13a in vitro by binding to Cas13a. Here, we solved the crystal structure of AcrVIA2 at a resolution of 2.59 A and confirmed that AcrVIA2 can bind to Helical-I domain in LshCas13a. Structural analysis show that the V-shaped acidic groove formed by beta3-beta3 hairpin of AcrVIA2 dimer is the key region that mediates the interaction between AcrVIA2 and Helical-I domain. In addition, we also reveal that Asp37 of AcrVIA2 plays an essential role in the functioning of the V-shaped acidic groove, and the functional dimer conformation of AcrVIA2 is stabilized by hydrogen bonds formed between Tyr41 of one monomer with Glu35 and Asp37 of the other monomer. These data expand the current understanding of the diverse interaction mechanisms between Acrs and Cas proteins, and also provide new ideas for the development of CRISPR-Cas13a regulatory tool.

-	~~Authors~~:	+	Structure of AcrVIA2 and its binding mechanism to CRISPR-Cas13a.,Song G, Li X, Wang Z, Dong C, Xie X, Yan X Biochem Biophys Res Commun. 2022 Jul 5;612:84-90. doi: , 10.1016/j.bbrc.2022.04.091. Epub 2022 Apr 23. PMID:35512461<ref>PMID:35512461</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7xmw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Leptotrichia wadei F0279]]
		+	[[Category: Li X]]
		+	[[Category: Song G]]
		+	[[Category: Yan X]]

OCA at 03:58, 8 June 2022

OCA — Wed, 08 Jun 2022 03:58:20 GMT

←Older revision		Revision as of 03:58, 8 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xmw is ON HOLD ~~until sometime in the future~~	+	The entry 7xmw is ON HOLD

	Authors:		Authors:

OCA at 04:55, 12 May 2022

OCA — Thu, 12 May 2022 04:55:39 GMT

←Older revision		Revision as of 04:55, 12 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xmw is ON HOLD	+	The entry 7xmw is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "7xmw" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2022 11:42:17 GMT

Protected "7xmw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:42, 4 May 2022

OCA: New page: '''Unreleased structure''' The entry 7xmw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 May 2022 11:42:16 GMT

New page: '''Unreleased structure''' The entry 7xmw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7xmw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7xmw - Revision history

OCA at 05:33, 31 May 2023

OCA at 03:58, 8 June 2022

OCA at 04:55, 12 May 2022

OCA: Protected "7xmw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7xmw is ON HOLD Authors: Description: Category: Unreleased Structures