7xsg - Revision history

OCA at 07:23, 3 April 2024

OCA — Wed, 03 Apr 2024 07:23:39 GMT

←Older revision		Revision as of 07:23, 3 April 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7xsg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cecembia_lonarensis_LW9 Cecembia lonarensis LW9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSG FirstGlance]. <br>		<table><tr><td colspan='2'>[[7xsg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cecembia_lonarensis_LW9 Cecembia lonarensis LW9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.609Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsg OCA], [https://pdbe.org/7xsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsg RCSB], [https://www.ebi.ac.uk/pdbsum/7xsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsg OCA], [https://pdbe.org/7xsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsg RCSB], [https://www.ebi.ac.uk/pdbsum/7xsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/K1KV82_9BACT K1KV82_9BACT]		[https://www.uniprot.org/uniprot/K1KV82_9BACT K1KV82_9BACT]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Glucose, a common monosaccharide in nature, is dominated by the d-enantiomer. Meanwhile, the discovery of l-glucose-utilizing bacteria and the elucidation of their metabolic pathways 10 years ago suggests that l-glucose exists naturally. Most carbohydrates exist as glycosides rather than monosaccharides; therefore, we expected that nature also contains l-glucosides. Sequence analysis within glycoside hydrolase family 29 led us to identify two alpha-l-glucosidases, ClAgl29A and ClAgl29B, derived from Cecembia lonarensis LW9. ClAgl29A and ClAgl29B exhibited higher K (m), k (cat), and k (cat)/K (m) values for p-nitrophenyl alpha-l-glucoside than that for p-nitrophenyl alpha-l-fucoside. Structural analysis of ClAgl29B in complex with l-glucose showed that these enzymes have an active-site pocket that preferentially binds alpha-l-glucoside, but excludes alpha-l-fucoside. These results suggest that ClAgl29A and ClAgl29B evolved to hydrolyze alpha-l-glucoside, implying the existence of alpha-l-glucoside in nature. Furthermore, alpha-l-glucosidic linkages (alpha-l-Glc-(1 --> 3)-l-Glc, alpha-l-Glc-(1 --> 2)-l-Glc, and alpha-l-Glc-(1 --> 6)-l-Glc) were synthesized by the transglucosylation activity of ClAgl29A and ClAgl29B. We believe that this study will lead to new research on alpha-l-glucosides, including determining the physiological effects on humans, and the discovery of novel alpha-l-glucoside-related enzymes.
-
-	Discovery of alpha-l-Glucosidase Raises the Possibility of alpha-l-Glucosides in Nature.,Shishiuchi R, Kang H, Tagami T, Ueda Y, Lang W, Kimura A, Okuyama M ACS Omega. 2022 Dec 9;7(50):47411-47423. doi: 10.1021/acsomega.2c06991. , eCollection 2022 Dec 20. PMID:36570207<ref>PMID:36570207</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7xsg" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:21, 18 January 2023

OCA — Wed, 18 Jan 2023 07:21:46 GMT

←Older revision		Revision as of 07:21, 18 January 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7xsg is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of ClAgl29B==
		+	<StructureSection load='7xsg' size='340' side='right'caption='[[7xsg]], [[Resolution\|resolution]] 1.61Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xsg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cecembia_lonarensis_LW9 Cecembia lonarensis LW9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsg OCA], [https://pdbe.org/7xsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsg RCSB], [https://www.ebi.ac.uk/pdbsum/7xsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K1KV82_9BACT K1KV82_9BACT]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glucose, a common monosaccharide in nature, is dominated by the d-enantiomer. Meanwhile, the discovery of l-glucose-utilizing bacteria and the elucidation of their metabolic pathways 10 years ago suggests that l-glucose exists naturally. Most carbohydrates exist as glycosides rather than monosaccharides; therefore, we expected that nature also contains l-glucosides. Sequence analysis within glycoside hydrolase family 29 led us to identify two alpha-l-glucosidases, ClAgl29A and ClAgl29B, derived from Cecembia lonarensis LW9. ClAgl29A and ClAgl29B exhibited higher K (m), k (cat), and k (cat)/K (m) values for p-nitrophenyl alpha-l-glucoside than that for p-nitrophenyl alpha-l-fucoside. Structural analysis of ClAgl29B in complex with l-glucose showed that these enzymes have an active-site pocket that preferentially binds alpha-l-glucoside, but excludes alpha-l-fucoside. These results suggest that ClAgl29A and ClAgl29B evolved to hydrolyze alpha-l-glucoside, implying the existence of alpha-l-glucoside in nature. Furthermore, alpha-l-glucosidic linkages (alpha-l-Glc-(1 --> 3)-l-Glc, alpha-l-Glc-(1 --> 2)-l-Glc, and alpha-l-Glc-(1 --> 6)-l-Glc) were synthesized by the transglucosylation activity of ClAgl29A and ClAgl29B. We believe that this study will lead to new research on alpha-l-glucosides, including determining the physiological effects on humans, and the discovery of novel alpha-l-glucoside-related enzymes.

-	~~Authors~~:	+	Discovery of alpha-l-Glucosidase Raises the Possibility of alpha-l-Glucosides in Nature.,Shishiuchi R, Kang H, Tagami T, Ueda Y, Lang W, Kimura A, Okuyama M ACS Omega. 2022 Dec 9;7(50):47411-47423. doi: 10.1021/acsomega.2c06991. , eCollection 2022 Dec 20. PMID:36570207<ref>PMID:36570207</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7xsg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cecembia lonarensis LW9]]
		+	[[Category: Large Structures]]
		+	[[Category: Kang H]]
		+	[[Category: Okuyama M]]
		+	[[Category: Shishiuchi R]]
		+	[[Category: Tagami T]]

OCA at 03:59, 8 June 2022

OCA — Wed, 08 Jun 2022 03:59:42 GMT

←Older revision		Revision as of 03:59, 8 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xsg is ON HOLD	+	The entry 7xsg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7xsg" [edit=sysop:move=sysop]

OCA — Wed, 25 May 2022 06:45:47 GMT

Protected "7xsg" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 25 May 2022

OCA: New page: '''Unreleased structure''' The entry 7xsg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 May 2022 06:45:46 GMT

New page: '''Unreleased structure''' The entry 7xsg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7xsg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]