7xsz - Revision history

OCA at 18:45, 7 September 2022

OCA — Wed, 07 Sep 2022 18:45:21 GMT

←Older revision		Revision as of 18:45, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]		[[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but its mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome, in the presence of transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT. The structures show snapshots of EC progression on DNA, mediating downstream nucleosome disassembly followed by its reassembly upstream of the EC, facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site, and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information.
		+
		+	Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.,Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI Science. 2022 Aug 18:eabp9466. doi: 10.1126/science.abp9466. PMID:35981082<ref>PMID:35981082</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7xsz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:40, 7 September 2022

OCA — Wed, 07 Sep 2022 16:40:21 GMT

←Older revision		Revision as of 16:40, 7 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7xsz is ~~ON HOLD until Paper Publication~~	+	==RNA polymerase II elongation complex transcribing a nucleosome (EC115)==
-		+	<StructureSection load='7xsz' size='340' side='right'caption='[[7xsz]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7xsz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii Komagataella phaffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsz OCA], [https://pdbe.org/7xsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsz RCSB], [https://www.ebi.ac.uk/pdbsum/7xsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Komagataella phaffii]]
		+	[[Category: Large Structures]]
		+	[[Category: Ehara H]]
		+	[[Category: Kujirai T]]
		+	[[Category: Kurumizaka H]]
		+	[[Category: Sekine S]]
		+	[[Category: Shirouzu M]]

OCA at 06:06, 29 June 2022

OCA — Wed, 29 Jun 2022 06:06:35 GMT

←Older revision		Revision as of 06:06, 29 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xsz is ON HOLD	+	The entry 7xsz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7xsz" [edit=sysop:move=sysop]

OCA — Wed, 25 May 2022 06:46:33 GMT

Protected "7xsz" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:46, 25 May 2022

OCA: New page: '''Unreleased structure''' The entry 7xsz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 May 2022 06:46:32 GMT

New page: '''Unreleased structure''' The entry 7xsz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7xsz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]