7xxy - Revision history

OCA at 18:03, 29 November 2023

OCA — Wed, 29 Nov 2023 18:03:15 GMT

←Older revision		Revision as of 18:03, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7xxy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XXY FirstGlance]. <br>		<table><tr><td colspan='2'>[[7xxy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XXY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.92Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xxy OCA], [https://pdbe.org/7xxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xxy RCSB], [https://www.ebi.ac.uk/pdbsum/7xxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xxy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xxy OCA], [https://pdbe.org/7xxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xxy RCSB], [https://www.ebi.ac.uk/pdbsum/7xxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xxy ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:28, 12 April 2023

OCA — Wed, 12 Apr 2023 20:28:23 GMT

←Older revision		Revision as of 20:28, 12 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7xxy is ~~ON HOLD until Paper~~ Publication	+	==Macaca mulatta galectin-10/Charcot-Leyden crystal protein with lactose==
		+	<StructureSection load='7xxy' size='340' side='right'caption='[[7xxy]], [[Resolution\|resolution]] 1.92Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7xxy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XXY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xxy OCA], [https://pdbe.org/7xxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xxy RCSB], [https://www.ebi.ac.uk/pdbsum/7xxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xxy ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Charcot-Leyden crystals (CLCs) are the hallmark of many eosinophilic-based diseases, such as asthma. Here, we report that reduced glutathione (GSH) disrupts CLCs and inhibits crystallization of human galectin-10 (Gal-10). GSH has no effect on CLCs from monkeys ( Macaca fascicularis or M. mulatta), even though monkey Gal-10s contain Cys29 and Cys32. Interestingly, human Gal-10 contains another cysteine residue (Cys57). Because GSH cannot disrupt CLCs formed by the human Gal-10 variant C57A or inhibit its crystallization, the effects of GSH on human Gal-10 or CLCs most likely occur by chemical modification of Cys57. We further report the crystal structures of Gal-10 from M. fascicularis and M. mulatta, along with their ability to bind to lactose and inhibit erythrocyte agglutination. Structural comparison with human Gal-10 shows that Cys57 and Gln75 within the ligand binding site are responsible for the loss of lactose binding. Pull-down experiments and mass spectrometry show that human Gal-10 interacts with tubulin alpha-1B, with GSH, GTP and Mg (2+) stabilizing this interaction and colchicine inhibiting it. Overall, this study enhances our understanding of Gal-10 function and CLC formation and suggests that GSH may be used as a pharmaceutical agent to ameliorate CLC-induced diseases.

-	~~Authors: Su~~, J.Y.	+	Glutathione disrupts galectin-10 Charcot-Leyden crystal formation to possibly ameliorate eosinophil-based diseases such as asthma.,Na H, Sayed H, Ayala GJ, Wang X, Liu Y, Yu J, Liu T, Mayo KH, Su J Acta Biochim Biophys Sin (Shanghai). 2023 Mar 25. doi: 10.3724/abbs.2023050. PMID:36988350<ref>PMID:36988350</ref>

-	~~Description: Macaca mulatta galectin-10~~/~~Charcot~~-~~Leyden crystal protein with lactose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Su~~, J.Y~~]]	+	<div class="pdbe-citations 7xxy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Macaca mulatta]]
		+	[[Category: Su JY]]

OCA at 03:57, 13 July 2022

OCA — Wed, 13 Jul 2022 03:57:22 GMT

←Older revision		Revision as of 03:57, 13 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7xxy is ON HOLD	+	The entry 7xxy is ON HOLD until Paper Publication

	Authors: Su, J.Y.		Authors: Su, J.Y.

OCA: Protected "7xxy" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2022 04:04:39 GMT

Protected "7xxy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:04, 8 June 2022

OCA: New page: '''Unreleased structure''' The entry 7xxy is ON HOLD Authors: Su, J.Y. Description: Macaca mulatta galectin-10/Charcot-Leyden crystal protein with lactose [[Category: Unreleased Struct...

OCA — Wed, 08 Jun 2022 04:04:38 GMT

New page: '''Unreleased structure''' The entry 7xxy is ON HOLD Authors: Su, J.Y. Description: Macaca mulatta galectin-10/Charcot-Leyden crystal protein with lactose [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 7xxy is ON HOLD

Authors: Su, J.Y.

Description: Macaca mulatta galectin-10/Charcot-Leyden crystal protein with lactose
[[Category: Unreleased Structures]]
[[Category: Su, J.Y]]