OCA at 10:32, 2 August 2023

OCA — Wed, 02 Aug 2023 10:32:59 GMT

←Older revision		Revision as of 10:32, 2 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7y0l is ~~ON HOLD until Paper~~ Publication	+	==SulE-S209A==
		+	<StructureSection load='7y0l' size='340' side='right'caption='[[7y0l]], [[Resolution\|resolution]] 1.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7y0l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hansschlegelia_zhihuaiae Hansschlegelia zhihuaiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y0L FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.29Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MM:METHYL+2-[({[(4-METHOXY-6-METHYL-1,3,5-TRIAZIN-2-YL)AMINO]CARBONYL}AMINO)SULFONYL]BENZOATE'>1MM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y0l OCA], [https://pdbe.org/7y0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y0l RCSB], [https://www.ebi.ac.uk/pdbsum/7y0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y0l ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G9I933_9HYPH G9I933_9HYPH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SulE, an esterase, which detoxifies a variety of sulfonylurea herbicides through de-esterification, provides an attractive approach to remove environmental sulfonylurea herbicides and develop herbicide-tolerant crops. Here, we determined the crystal structures of SulE and an activity improved mutant P44R. Structural analysis revealed that SulE is a dimer with spacious binding pocket accommodating the large sulfonylureas substrate. Particularly, SulE contains a protruding beta hairpin with a lid loop covering the active site of the other subunit of the dimer. The lid loop participates in substrate recognition and binding. P44R mutation altered the lid loop flexibility, resulting in the sulfonylurea heterocyclic ring repositioning to a relative stable conformation thus leading to dramatically increased activity. Our work provides important insights into the molecular mechanism of SulE, and establish a solid foundation for further improving the enzyme activity to various sulfonylurea herbicides through rational design.

-	~~Authors: Liu~~, B., ~~Ran~~, T., He, J.~~, Wang, W~~.	+	Crystal structures of herbicide-detoxifying esterase reveal a lid loop affecting substrate binding and activity.,Liu B, Wang W, Qiu J, Huang X, Qiu S, Bao Y, Xu S, Ruan L, Ran T, He J Nat Commun. 2023 Jul 19;14(1):4343. doi: 10.1038/s41467-023-40103-5. PMID:37468532<ref>PMID:37468532</ref>

-	~~Description: SulE~~-~~S209A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Wang, W~~]]	+	<div class="pdbe-citations 7y0l" style="background-color:#fffaf0;"></div>
-	[[Category: He, J]]	+	== References ==
-	[[Category: Liu, B]]	+	<references/>
-	[[Category: Ran, T]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Hansschlegelia zhihuaiae]]
		+	[[Category: Large Structures]]
		+	[[Category: He J]]
		+	[[Category: Liu B]]
		+	[[Category: Ran T]]
		+	[[Category: Wang W]]

OCA at 03:57, 13 July 2022

OCA — Wed, 13 Jul 2022 03:57:52 GMT

←Older revision		Revision as of 03:57, 13 July 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7y0l is ON HOLD	+	The entry 7y0l is ON HOLD until Paper Publication

	Authors: Liu, B., Ran, T., He, J., Wang, W.		Authors: Liu, B., Ran, T., He, J., Wang, W.

OCA: Protected "7y0l" [edit=sysop:move=sysop]

OCA — Wed, 15 Jun 2022 03:57:08 GMT

Protected "7y0l" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:57, 15 June 2022

OCA: New page: '''Unreleased structure''' The entry 7y0l is ON HOLD Authors: Liu, B., Ran, T., He, J., Wang, W. Description: SulE-S209A Category: Unreleased Structures Category: Wang, W [[Cat...

OCA — Wed, 15 Jun 2022 03:57:07 GMT

New page: '''Unreleased structure''' The entry 7y0l is ON HOLD Authors: Liu, B., Ran, T., He, J., Wang, W. Description: SulE-S209A Category: Unreleased Structures Category: Wang, W [[Cat...

New page

'''Unreleased structure'''

The entry 7y0l is ON HOLD

Authors: Liu, B., Ran, T., He, J., Wang, W.

Description: SulE-S209A
[[Category: Unreleased Structures]]
[[Category: Wang, W]]
[[Category: He, J]]
[[Category: Liu, B]]
[[Category: Ran, T]]

7y0l - Revision history

OCA at 10:32, 2 August 2023

OCA at 03:57, 13 July 2022

OCA: Protected "7y0l" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7y0l is ON HOLD Authors: Liu, B., Ran, T., He, J., Wang, W. Description: SulE-S209A Category: Unreleased Structures Category: Wang, W [[Cat...