OCA at 10:06, 14 December 2022

OCA — Wed, 14 Dec 2022 10:06:12 GMT

←Older revision		Revision as of 10:06, 14 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7y5g is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of a eukaryotic ZnT8 in the presence of zinc==
		+	<StructureSection load='7y5g' size='340' side='right'caption='[[7y5g]], [[Resolution\|resolution]] 3.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7y5g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_tropicalis Xenopus tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y5G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y5g OCA], [https://pdbe.org/7y5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y5g RCSB], [https://www.ebi.ac.uk/pdbsum/7y5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y5g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ZNT8_XENTR ZNT8_XENTR] Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. Involved in zinc ion homeostasis and cellular distribution (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Zinc transporter 8 (ZnT8) is mainly expressed in pancreatic islet beta cells and is responsible for H(+)-coupled uptake (antiport) of Zn(2+) into the lumen of insulin secretory granules. Structures of human ZnT8 and its prokaryotic homolog YiiP have provided structural basis for constructing a plausible transport cycle for Zn(2+). However, the mechanistic role that protons play in the transport process remains unclear. Here we present a lumen-facing cryo-EM structure of ZnT8 from Xenopus tropicalis (xtZnT8) in the presence of Zn(2+) at a luminal pH (5.5). Compared to a Zn(2+)-bound xtZnT8 structure at a cytosolic pH (7.5), the low-pH structure displays an empty transmembrane Zn(2+)-binding site with a disrupted coordination geometry. Combined with a Zn(2+)-binding assay our data suggest that protons may disrupt Zn(2+) coordination at the transmembrane Zn(2+)-binding site in the lumen-facing state, thus facilitating Zn(2+) release from ZnT8 into the lumen.

-	~~Authors: Zhang~~, S., Fu, C., Luo, Y., ~~Sun~~, Z., Su, Z., Zhou, X.	+	Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn(2+)-releasing mechanism.,Zhang S, Fu C, Luo Y, Xie Q, Xu T, Sun Z, Su Z, Zhou X J Struct Biol. 2022 Dec 1;215(1):107926. doi: 10.1016/j.jsb.2022.107926. PMID:36464198<ref>PMID:36464198</ref>

-	~~Description: Cryo-EM structure of~~ a ~~eukaryotic ZnT8 in~~ the ~~presence~~ of ~~zinc~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Zhou, X~~]]	+	<div class="pdbe-citations 7y5g" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Su, Z~~]]	+	== References ==
-	[[Category: Luo, Y]]	+	<references/>
-	[[Category: ~~Zhang, S~~]]	+	__TOC__
-	[[Category: Sun, Z]]	+	</StructureSection>
-	[[Category: ~~Fu, C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Xenopus tropicalis]]
		+	[[Category: Fu C]]
		+	[[Category: Luo Y]]
		+	[[Category: Su Z]]
		+	[[Category: Sun Z]]
		+	[[Category: Zhang S]]
		+	[[Category: Zhou X]]

OCA at 06:08, 29 June 2022

OCA — Wed, 29 Jun 2022 06:08:08 GMT

←Older revision		Revision as of 06:08, 29 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7y5g is ON HOLD	+	The entry 7y5g is ON HOLD until Paper Publication

	Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X.		Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X.
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	[[Category: Luo, Y]]		[[Category: Luo, Y]]
	[[Category: Zhang, S]]		[[Category: Zhang, S]]
-	[[Category: Fu, C]]
	[[Category: Sun, Z]]		[[Category: Sun, Z]]
		+	[[Category: Fu, C]]

OCA: Protected "7y5g" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2022 07:07:35 GMT

Protected "7y5g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:07, 22 June 2022

OCA: New page: '''Unreleased structure''' The entry 7y5g is ON HOLD Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X. Description: Cryo-EM structure of a eukaryotic ZnT8 in the presence ...

OCA — Wed, 22 Jun 2022 07:07:34 GMT

New page: '''Unreleased structure''' The entry 7y5g is ON HOLD Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X. Description: Cryo-EM structure of a eukaryotic ZnT8 in the presence ...

New page

'''Unreleased structure'''

The entry 7y5g is ON HOLD

Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X.

Description: Cryo-EM structure of a eukaryotic ZnT8 in the presence of zinc
[[Category: Unreleased Structures]]
[[Category: Zhou, X]]
[[Category: Su, Z]]
[[Category: Luo, Y]]
[[Category: Zhang, S]]
[[Category: Fu, C]]
[[Category: Sun, Z]]

7y5g - Revision history

OCA at 10:06, 14 December 2022

OCA at 06:08, 29 June 2022

OCA: Protected "7y5g" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7y5g is ON HOLD Authors: Zhang, S., Fu, C., Luo, Y., Sun, Z., Su, Z., Zhou, X. Description: Cryo-EM structure of a eukaryotic ZnT8 in the presence ...