OCA at 10:10, 24 November 2022

OCA — Thu, 24 Nov 2022 10:10:29 GMT

←Older revision		Revision as of 10:10, 24 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7y6i is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of human sodium-chloride cotransporter==
		+	<StructureSection load='7y6i' size='340' side='right'caption='[[7y6i]], [[Resolution\|resolution]] 2.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7y6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y6I FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LPE:1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>LPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y6i OCA], [https://pdbe.org/7y6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y6i RCSB], [https://www.ebi.ac.uk/pdbsum/7y6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y6i ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/S12A3_HUMAN S12A3_HUMAN] Gitelman syndrome. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/S12A3_HUMAN S12A3_HUMAN] Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (PubMed:21613606, PubMed:22009145). Receptor for the pro-inflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).[UniProtKB:P59158]<ref>PMID:21613606</ref> <ref>PMID:22009145</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The sodium-chloride cotransporter NCC mediates the coupled import of sodium and chloride across the plasma membrane, playing vital roles in kidney extracellular fluid volume and blood pressure control. Here, we present the full-length structure of human NCC, with 2.9 A for the transmembrane domain and 3.8 A for the carboxyl-terminal domain. NCC adopts an inward-open conformation and a domain-swap dimeric assembly. Conserved ion binding sites among the cation-chloride cotransporters and the Na2 site are observed in our structure. A unique His residue in the substrate pocket in NCC potentially interacts with Na1 and Cl1 and might also mediate the coordination of Na2 through a Ser residue. Putative observed water molecules are indicated to participate in the coordination of ions and TM coupling. Together with transport activity assays, our structure provides the first glimpse of NCC and defines ion binding sites, promoting drug development for hypertension targeting on NCC.

-	~~Authors~~:	+	Cryo-EM structure of the human sodium-chloride cotransporter NCC.,Nan J, Yuan Y, Yang X, Shan Z, Liu H, Wei F, Zhang W, Zhang Y Sci Adv. 2022 Nov 11;8(45):eadd7176. doi: 10.1126/sciadv.add7176. Epub 2022 Nov , 9. PMID:36351028<ref>PMID:36351028</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7y6i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Nan J]]
		+	[[Category: Shan Z]]
		+	[[Category: Yang X]]
		+	[[Category: Yuan Y]]
		+	[[Category: Zhang YQ]]

OCA at 14:55, 6 July 2022

OCA — Wed, 06 Jul 2022 14:55:53 GMT

←Older revision		Revision as of 14:55, 6 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7y6i is ON HOLD	+	The entry 7y6i is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7y6i" [edit=sysop:move=sysop]

OCA — Wed, 29 Jun 2022 06:09:14 GMT

Protected "7y6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:09, 29 June 2022

OCA: New page: '''Unreleased structure''' The entry 7y6i is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Jun 2022 06:09:14 GMT

New page: '''Unreleased structure''' The entry 7y6i is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7y6i is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7y6i - Revision history

OCA at 10:10, 24 November 2022

OCA at 14:55, 6 July 2022

OCA: Protected "7y6i" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7y6i is ON HOLD Authors: Description: Category: Unreleased Structures