OCA at 07:11, 12 July 2023

OCA — Wed, 12 Jul 2023 07:11:15 GMT

←Older revision		Revision as of 07:11, 12 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7ye6 is ~~ON HOLD~~	+	==BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc==
		+	<StructureSection load='7ye6' size='340' side='right'caption='[[7ye6]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7ye6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YE6 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ye6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ye6 OCA], [https://pdbe.org/7ye6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ye6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ye6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ye6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BAMA_ECOLI BAMA_ECOLI] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery.<ref>PMID:15951436</ref> <ref>PMID:16102012</ref> <ref>PMID:16824102</ref> <ref>PMID:20378773</ref> <ref>PMID:21823654</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The outer membrane structure is common in Gram-negative bacteria, mitochondria and chloroplasts, and contains outer membrane beta-barrel proteins (OMPs) that are essential interchange portals of materials(1-3). All known OMPs share the antiparallel beta-strand topology(4), implicating a common evolutionary origin and conserved folding mechanism. Models have been proposed for bacterial beta-barrel assembly machinery (BAM) to initiate OMP folding(5,6); however, mechanisms by which BAM proceeds to complete OMP assembly remain unclear. Here we report intermediate structures of BAM assembling an OMP substrate, EspP, demonstrating sequential conformational dynamics of BAM during the late stages of OMP assembly, which is further supported by molecular dynamics simulations. Mutagenic in vitro and in vivo assembly assays reveal functional residues of BamA and EspP for barrel hybridization, closure and release. Our work provides novel insights into the common mechanism of OMP assembly.

-	~~Authors~~:	+	Structural basis of BAM-mediated outer membrane beta-barrel protein assembly.,Shen C, Chang S, Luo Q, Chan KC, Zhang Z, Luo B, Xie T, Lu G, Zhu X, Wei X, Dong C, Zhou R, Zhang X, Tang X, Dong H Nature. 2023 May;617(7959):185-193. doi: 10.1038/s41586-023-05988-8. Epub 2023 , Apr 26. PMID:37100902<ref>PMID:37100902</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7ye6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Escherichia coli O157:H7]]
		+	[[Category: Large Structures]]
		+	[[Category: Chang S]]
		+	[[Category: Dong C]]
		+	[[Category: Dong H]]
		+	[[Category: Lu G]]
		+	[[Category: Luo B]]
		+	[[Category: Luo Q]]
		+	[[Category: Shen C]]
		+	[[Category: Tang X]]
		+	[[Category: Wei X]]
		+	[[Category: Zhang X]]
		+	[[Category: Zhang Z]]
		+	[[Category: Zhu X]]

OCA: Protected "7ye6" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2022 04:02:04 GMT

Protected "7ye6" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:02, 13 July 2022

OCA: New page: '''Unreleased structure''' The entry 7ye6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Jul 2022 04:02:02 GMT

New page: '''Unreleased structure''' The entry 7ye6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ye6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ye6 - Revision history

OCA at 07:11, 12 July 2023

OCA: Protected "7ye6" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ye6 is ON HOLD Authors: Description: Category: Unreleased Structures