OCA at 11:18, 1 February 2023

OCA — Wed, 01 Feb 2023 11:18:08 GMT

←Older revision		Revision as of 11:18, 1 February 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7yjr is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of MCR-1-S treated by sodium aurothiomalate==
		+	<StructureSection load='7yjr' size='340' side='right'caption='[[7yjr]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7yjr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjr OCA], [https://pdbe.org/7yjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjr RCSB], [https://www.ebi.ac.uk/pdbsum/7yjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MCR1_ECOLX MCR1_ECOLX] Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A gives polymyxin resistance (PubMed:26603172).<ref>PMID:26603172</ref> Confers resistance to polymyxin-type antibiotics; expression of the Mcr-1 protein in E.coli increases colistin and polymyxin B minimal inhibitory concentration (MIC) from 0.5 mg/ml to 2.0 mg/ml. The pHNSHP45 plasmid can transfer efficiently (0.1 to 0.001) to other E.coli strains by conjugation and increases polymxin MIC by 8- to 16-fold; it may not require selective pressure to be maintained in the cell. When transformed into K.pneumoniae or P.aeruginosa it also increases polymxin MIC 8- to 16-fold. In a murine (BALB/c mice) thigh infection study using an mcr1-encoding plasmid isolated from a human patient, the plasmid confers in vivo protection against colistin (PubMed:26603172).<ref>PMID:26603172</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The emergence and rapid spread of the mobile colistin resistance gene mcr-1 among bacterial species and hosts significantly challenge the efficacy of "last-line" antibiotic colistin. Previously, we reported silver nitrate and auranofin serve as colistin adjuvants for combating mcr-1-positive bacteria. Herein, we uncovered more gold-based drugs and nanoparticles, and found that they exhibited varying degree of synergisms with colistin on killing mcr-1-positive bacteria. However, pre-activation of the drugs by either glutathione or N-acetyl cysteine, thus releasing and accumulating gold ions, is perquisite for their abilities to substitute zinc cofactor from MCR-1 enzyme. X-ray crystallography and biophysical studies further supported the proposed mechanism. This study not only provides basis for combining gold-based drugs and colistin for combating mcr-1-positive bacterial infections, but also undoubtedly opens a new horizon for metabolism details of gold-based drugs in overcoming antimicrobial resistance.

-	~~Authors~~:	+	Gold drugs as colistin adjuvants in the fight against MCR-1 producing bacteria.,Zhang Q, Wang M, Hu X, Yan A, Ho PL, Li H, Sun H J Biol Inorg Chem. 2023 Jan 20. doi: 10.1007/s00775-022-01983-y. PMID:36662362<ref>PMID:36662362</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7yjr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Sun H]]
		+	[[Category: Wang M]]
		+	[[Category: Zhang Q]]

OCA at 03:44, 10 August 2022

OCA — Wed, 10 Aug 2022 03:44:12 GMT

←Older revision		Revision as of 03:44, 10 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yjr is ON HOLD	+	The entry 7yjr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7yjr" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2022 16:28:29 GMT

Protected "7yjr" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:28, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 7yjr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2022 16:28:28 GMT

New page: '''Unreleased structure''' The entry 7yjr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7yjr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7yjr - Revision history

OCA at 11:18, 1 February 2023

OCA at 03:44, 10 August 2022

OCA: Protected "7yjr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7yjr is ON HOLD Authors: Description: Category: Unreleased Structures