OCA at 12:34, 26 July 2023

OCA — Wed, 26 Jul 2023 12:34:17 GMT

←Older revision		Revision as of 12:34, 26 July 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ykc is ~~ON HOLD~~	+	==crystal structure of the Phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (ARO3) from Saccharomyces cerevisiae==
		+	<StructureSection load='7ykc' size='340' side='right'caption='[[7ykc]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7ykc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YKC FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ykc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ykc OCA], [https://pdbe.org/7ykc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ykc RCSB], [https://www.ebi.ac.uk/pdbsum/7ykc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ykc ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AROF_YEAST AROF_YEAST] Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key enzyme in the shikimate pathway for the biosynthesis of aromatic compounds. (L)-Phe and (L)-Tyr bind to the two main DAHPS isoforms and inhibit their enzyme activities, respectively. Synthetic biologists aim to relieve such inhibitions in order to improve the productivity of aromatic compounds. In this work, we reported a point mutant of yeast DHAPS, Aro3(D154N), which retains the wild type enzyme activity but converts it highly inert to the inhibition by (L)-Phe. The Aro3 crystal structure along with the molecular dynamics simulations analysis suggests that the D154N mutation distant from the inhibitor binding cavity may reduce the binding affinity of (L)-Phe. Growth assays demonstrated that substitution of the conserved D154 with asparagine suffices to relieve the inhibition of (L)-Phe on Aro3, (L)-Tyr on Aro4, and the inhibitions on their corresponding homologues from diverse yeasts. The importance of our discovery is highlighted by the observation of 29.1% and 43.6% increase of yield for the production of tyrosol and salidroside respectively upon substituting ARO3 with ARO3(D154N). We anticipate that this allele would be used broadly to increase the yield of various aromatic products in metabolically diverse microorganisms.

-	~~Authors:~~ Liu, H., ~~Luo~~, Y.	+	Mechanistic investigation of a D to N mutation in DAHP synthase that dictates carbon flux into the shikimate pathway in yeast.,Liu H, Xiao Q, Wu X, Ma H, Li J, Guo X, Liu Z, Zhang Y, Luo Y Commun Chem. 2023 Jul 15;6(1):152. doi: 10.1038/s42004-023-00946-x. PMID:37454208<ref>PMID:37454208</ref>

-	~~Description: crystal structure~~ of the ~~Phenylalanine~~-~~regulated 3~~-~~deoxy-D-arabino-heptulosonate-7-phosphate synthase (ARO3) from Saccharomyces cerevisiae~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Luo, Y~~]]	+	<div class="pdbe-citations 7ykc" style="background-color:#fffaf0;"></div>
-	[[Category: Liu, H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Liu H]]
		+	[[Category: Luo Y]]

OCA at 03:44, 12 July 2023

OCA — Wed, 12 Jul 2023 03:44:23 GMT

←Older revision		Revision as of 03:44, 12 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ykc is ON HOLD ~~until 2024-07-22~~	+	The entry 7ykc is ON HOLD

	Authors: Liu, H., Luo, Y.		Authors: Liu, H., Luo, Y.

OCA at 03:34, 25 August 2022

OCA — Thu, 25 Aug 2022 03:34:14 GMT

←Older revision		Revision as of 03:34, 25 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ykc is ON HOLD	+	The entry 7ykc is ON HOLD until 2024-07-22

	Authors: Liu, H., Luo, Y.		Authors: Liu, H., Luo, Y.

OCA: Protected "7ykc" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2022 16:29:32 GMT

Protected "7ykc" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:29, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 7ykc is ON HOLD Authors: Liu, H., Luo, Y. Description: crystal structure of the Phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate...

OCA — Wed, 27 Jul 2022 16:29:31 GMT

New page: '''Unreleased structure''' The entry 7ykc is ON HOLD Authors: Liu, H., Luo, Y. Description: crystal structure of the Phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate...

New page

'''Unreleased structure'''

The entry 7ykc is ON HOLD

Authors: Liu, H., Luo, Y.

Description: crystal structure of the Phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (ARO3) from Saccharomyces cerevisiae
[[Category: Unreleased Structures]]
[[Category: Luo, Y]]
[[Category: Liu, H]]

7ykc - Revision history

OCA at 12:34, 26 July 2023

OCA at 03:44, 12 July 2023

OCA at 03:34, 25 August 2022

OCA: Protected "7ykc" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ykc is ON HOLD Authors: Liu, H., Luo, Y. Description: crystal structure of the Phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate...