7ypv - Revision history

OCA at 18:11, 29 November 2023

OCA — Wed, 29 Nov 2023 18:11:07 GMT

←Older revision		Revision as of 18:11, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ypv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lavendulae_subsp._lavendulae Streptomyces lavendulae subsp. lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPV FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ypv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lavendulae_subsp._lavendulae Streptomyces lavendulae subsp. lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OI9:Streptothricin+F'>OI9</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.415Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OI9:Streptothricin+F'>OI9</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypv OCA], [https://pdbe.org/7ypv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypv RCSB], [https://www.ebi.ac.uk/pdbsum/7ypv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypv OCA], [https://pdbe.org/7ypv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypv RCSB], [https://www.ebi.ac.uk/pdbsum/7ypv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypv ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:36, 31 May 2023

OCA — Wed, 31 May 2023 05:36:24 GMT

←Older revision		Revision as of 05:36, 31 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7ypv is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of OrE-ST-F==
		+	<StructureSection load='7ypv' size='340' side='right'caption='[[7ypv]], [[Resolution\|resolution]] 2.42Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7ypv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lavendulae_subsp._lavendulae Streptomyces lavendulae subsp. lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OI9:Streptothricin+F'>OI9</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypv OCA], [https://pdbe.org/7ypv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypv RCSB], [https://www.ebi.ac.uk/pdbsum/7ypv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypv ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G9MBU1_STRLA G9MBU1_STRLA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a noncanonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycinothricin to form the antibiotic BD-12. X-ray crystallography was used to investigate this complex enzymatic process, which showed Orf1 has two substrate-binding sites that sit 13.5 A apart unlike canonical FAD-dependent oxidoreductases. One site could accommodate glycine and the other glycinothricin or glycylthricin. Moreover, an intermediate-enzyme adduct with a NOS-covalent linkage was observed in the later site, where it acts as a two-scissile-bond linkage facilitating nucleophilic addition and cofactor-free decarboxylation. The chain length of nucleophilic acceptors vies with bond cleavage sites at either N-O or O-S accounting for N-formimidoylation or N-iminoacetylation. The resultant product is no longer sensitive to aminoglycoside-modifying enzymes, a strategy that antibiotic-producing species employ to counter drug resistance in competing species.

-	~~Authors~~:	+	N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry.,Wang YL, Chang CY, Hsu NS, Lo IW, Lin KH, Chen CL, Chang CF, Wang ZC, Ogasawara Y, Dairi T, Maruyama C, Hamano Y, Li TL Nat Commun. 2023 May 3;14(1):2528. doi: 10.1038/s41467-023-38218-w. PMID:37137912<ref>PMID:37137912</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7ypv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptomyces lavendulae subsp. lavendulae]]
		+	[[Category: Li TL]]
		+	[[Category: Wang YL]]

OCA at 03:32, 18 August 2022

OCA — Thu, 18 Aug 2022 03:32:02 GMT

←Older revision		Revision as of 03:32, 18 August 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ypv is ON HOLD	+	The entry 7ypv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ypv" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2022 03:49:23 GMT

Protected "7ypv" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:49, 10 August 2022

OCA: New page: '''Unreleased structure''' The entry 7ypv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Aug 2022 03:49:22 GMT

New page: '''Unreleased structure''' The entry 7ypv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ypv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]