OCA at 21:14, 28 June 2023

OCA — Wed, 28 Jun 2023 21:14:20 GMT

←Older revision		Revision as of 21:14, 28 June 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7yvp is ~~ON HOLD~~	+	==Omicron BA.4/5 SARS-CoV-2 S in complex with TH272/281 Fab==
		+	<StructureSection load='7yvp' size='340' side='right'caption='[[7yvp]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7yvp]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YVP FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yvp OCA], [https://pdbe.org/7yvp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yvp RCSB], [https://www.ebi.ac.uk/pdbsum/7yvp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yvp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SARS-CoV-2 Omicron variant evades most currently approved neutralizing antibodies (nAbs) and caused drastic decrease of plasma neutralizing activity elicited by vaccination or prior infection, urging the need for the development of pan-variant antivirals. Breakthrough infection induces a hybrid immunological response with potentially broad, potent and durable protection against variants, therefore, convalescent plasma from breakthrough infection may provide a broadened repertoire for identifying elite nAbs. We performed single-cell RNA sequencing (scRNA-seq) and BCR sequencing (scBCR-seq) of B cells from BA.1 breakthrough-infected patients who received 2 or 3 previous doses of inactivated vaccine. Elite nAbs, mainly derived from the IGHV2-5 and IGHV3-66/53 germlines, showed potent neutralizing activity across Wuhan-Hu-1, Delta, Omicron sublineages BA.1 and BA.2 at picomolar NT(50) values. Cryo-EM analysis revealed diverse modes of spike recognition and guides the design of cocktail therapy. A single injection of paired antibodies cocktail provided potent protection in the K18-hACE2 transgenic female mouse model of SARS-CoV-2 infection.

-	~~Authors: Guo~~, Y., Zhang, G., Liang, J., Liu, F., ~~Rao~~, Z.	+	Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection.,Guo Y, Zhang G, Yang Q, Xie X, Lu Y, Cheng X, Wang H, Liang J, Tang J, Gao Y, Shang H, Dai J, Shi Y, Zhou J, Zhou J, Guo H, Yang H, Qi J, Liu L, Ma S, Zhang B, Huo Q, Xie Y, Wu J, Dong F, Zhang S, Lou Z, Gao Y, Song Z, Wang W, Sun Z, Yang X, Xiong D, Liu F, Chen X, Zhu P, Wang X, Cheng T, Rao Z Nat Commun. 2023 Jun 15;14(1):3537. doi: 10.1038/s41467-023-39267-x. PMID:37322000<ref>PMID:37322000</ref>

-	~~Description: Omicron BA~~.4/~~5 SARS~~-~~CoV~~-~~2 S in complex with TH272~~/~~281 Fab~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Liu, F~~]]	+	<div class="pdbe-citations 7yvp" style="background-color:#fffaf0;"></div>
-	[[Category: Guo, Y]]	+	== References ==
-	[[Category: Liang, J]]	+	<references/>
-	[[Category: Rao, Z]]	+	__TOC__
-	[[Category: Zhang, G]]	+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Guo Y]]
		+	[[Category: Liang J]]
		+	[[Category: Liu F]]
		+	[[Category: Rao Z]]
		+	[[Category: Zhang G]]

OCA at 05:33, 14 September 2022

OCA — Wed, 14 Sep 2022 05:33:39 GMT

←Older revision		Revision as of 05:33, 14 September 2022
Line 3:		Line 3:
	The entry 7yvp is ON HOLD		The entry 7yvp is ON HOLD

-	Authors: ~~Liu~~, F., Zhang, G., Liang, J., ~~Rao~~, Z., ~~Guo~~, Y.	+	Authors: Guo, Y., Zhang, G., Liang, J., Liu, F., Rao, Z.

-	Description: Omicron BA.5 SARS-CoV-2 S in complex with TH272/281 Fab	+	Description: Omicron BA.4/5 SARS-CoV-2 S in complex with TH272/281 Fab
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Guo, Y]]
-	[[Category: Rao, Z]]
	[[Category: Liu, F]]		[[Category: Liu, F]]
		+	[[Category: Guo, Y]]
	[[Category: Liang, J]]		[[Category: Liang, J]]
		+	[[Category: Rao, Z]]
	[[Category: Zhang, G]]		[[Category: Zhang, G]]

OCA: Protected "7yvp" [edit=sysop:move=sysop]

OCA — Thu, 25 Aug 2022 03:40:33 GMT

Protected "7yvp" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:40, 25 August 2022

OCA: New page: '''Unreleased structure''' The entry 7yvp is ON HOLD Authors: Liu, F., Zhang, G., Liang, J., Rao, Z., Guo, Y. Description: Omicron BA.5 SARS-CoV-2 S in complex with TH272/281 Fab [[Cat...

OCA — Thu, 25 Aug 2022 03:40:32 GMT

New page: '''Unreleased structure''' The entry 7yvp is ON HOLD Authors: Liu, F., Zhang, G., Liang, J., Rao, Z., Guo, Y. Description: Omicron BA.5 SARS-CoV-2 S in complex with TH272/281 Fab [[Cat...

New page

'''Unreleased structure'''

The entry 7yvp is ON HOLD

Authors: Liu, F., Zhang, G., Liang, J., Rao, Z., Guo, Y.

Description: Omicron BA.5 SARS-CoV-2 S in complex with TH272/281 Fab
[[Category: Unreleased Structures]]
[[Category: Guo, Y]]
[[Category: Rao, Z]]
[[Category: Liu, F]]
[[Category: Liang, J]]
[[Category: Zhang, G]]

7yvp - Revision history

OCA at 21:14, 28 June 2023

OCA at 05:33, 14 September 2022

OCA: Protected "7yvp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7yvp is ON HOLD Authors: Liu, F., Zhang, G., Liang, J., Rao, Z., Guo, Y. Description: Omicron BA.5 SARS-CoV-2 S in complex with TH272/281 Fab [[Cat...