7yx8 - Revision history

OCA at 05:04, 8 September 2022

OCA — Thu, 08 Sep 2022 05:04:52 GMT

←Older revision		Revision as of 05:04, 8 September 2022
Line 1:		Line 1:

-	====	+	==Crystal structure of the AM0627 (E326A) inactive mutant in complex with PSGL-1-like bis-T glycopeptide and Zn2+==
-	<StructureSection load='7yx8' size='340' side='right'caption='[[7yx8]]' scene=''>	+	<StructureSection load='7yx8' size='340' side='right'caption='[[7yx8]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7yx8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Akkermansia_muciniphila Akkermansia muciniphila] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YX8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yx8 OCA], [https://pdbe.org/7yx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yx8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yx8 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yx8 OCA], [https://pdbe.org/7yx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yx8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yx8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/B2UPI7_AKKM8 B2UPI7_AKKM8]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mucinases of human gut bacteria cleave peptide bonds in mucins strictly depending on the presence of neighboring O-glycans. The Akkermansia muciniphila AM0627 mucinase cleaves specifically in between contiguous (bis) O-glycans of defined truncated structures, suggesting that this enzyme may recognize clustered O-glycan patches. Here, we report the structure and molecular mechanism of AM0627 in complex with a glycopeptide containing a bis-T (Galbeta1-3GalNAcalpha1-O-Ser/Thr) O-glycan, revealing that AM0627 recognizes both the sugar moieties and the peptide sequence. AM0627 exhibits preference for bis-T over bis-Tn (GalNAcalpha1-O-Ser/Thr) O-glycopeptide substrates, with the first GalNAc residue being essential for cleavage. AM0627 follows a mechanism relying on a nucleophilic water molecule and a catalytic base Glu residue. Structural comparison among mucinases identifies a conserved Tyr engaged in sugar-pi interactions in both AM0627 and the Bacteroides thetaiotaomicron BT4244 mucinase as responsible for the common activity of these two mucinases with bis-T/Tn substrates. Our work illustrates how mucinases through tremendous flexibility adapt to the diversity in distribution and patterns of O-glycans on mucins.
		+
		+	Structural and mechanistic insights into the cleavage of clustered O-glycan patches-containing glycoproteins by mucinases of the human gut.,Taleb V, Liao Q, Narimatsu Y, Garcia-Garcia A, Companon I, Borges RJ, Gonzalez-Ramirez AM, Corzana F, Clausen H, Rovira C, Hurtado-Guerrero R Nat Commun. 2022 Jul 26;13(1):4324. doi: 10.1038/s41467-022-32021-9. PMID:35882872<ref>PMID:35882872</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7yx8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Akkermansia muciniphila]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Borges RJ]]
		+	[[Category: Clausen H]]
		+	[[Category: Companon I]]
		+	[[Category: Corzana F]]
		+	[[Category: Garcia-Garcia A]]
		+	[[Category: Gonzalez-Ramirez AM]]
		+	[[Category: Hurtado-Guerrero R]]
		+	[[Category: Liao Q]]
		+	[[Category: Narimatsu Y]]
		+	[[Category: Rovira C]]
		+	[[Category: Taleb V]]

OCA at 06:57, 20 July 2022

OCA — Wed, 20 Jul 2022 06:57:38 GMT

←Older revision		Revision as of 06:57, 20 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7yx8 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7yx8' size='340' side='right'caption='[[7yx8]]' scene=''>
-	~~Authors~~: ~~Taleb, V~~.~~, Liao, Q~~.~~, Narimatsu, Y~~.~~, Garcia~~-~~Garcia, A~~.~~, Companon, I~~.~~, Borges, R~~.J.~~, Gonzalez-Ramirez, A~~.M., ~~Corzana, F~~.~~, Clausen, H~~.~~, Rovira, C~~.~~, Hurtado~~-~~Guerrero~~, R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of the AM0627 (E326A) inactive mutant in complex with PSGL-1-like bis-T glycopeptide and Zn2+~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yx8 OCA], [https://pdbe.org/7yx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yx8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yx8 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	~~[[Category: Borges~~, ~~R.J]]~~	+	__TOC__
-	[~~[Category~~: ~~Gonzalez-Ramirez, A~~.M]]	+	</StructureSection>
-	~~[[Category: Hurtado-Guerrero~~, ~~R]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Taleb, V~~]]	+	[[Category: Z-disk]]
-	~~[[Category: Clausen~~, ~~H]]~~	+
-	[~~[Category~~: ~~Narimatsu, Y]]~~	+
-	~~[[Category: Garcia~~-~~Garcia, A]~~]	+
-	~~[[Category: Corzana, F]]~~	+
-	~~[[Category: Companon, I]]~~	+
-	[[Category: ~~Rovira, C~~]]	+
-	[[Category: ~~Liao, Q~~]]	+

OCA at 10:37, 27 April 2022

OCA — Wed, 27 Apr 2022 10:37:07 GMT

←Older revision		Revision as of 10:37, 27 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yx8 is ON HOLD	+	The entry 7yx8 is ON HOLD until Paper Publication

-	Authors: Taleb, V., Liao, Q., Narimatsu, Y., Garcia-Garcia, A., Companon, I., Borges, R.J., Corzana, F., Clausen, H., Rovira, C., Hurtado-Guerrero, R.	+	Authors: Taleb, V., Liao, Q., Narimatsu, Y., Garcia-Garcia, A., Companon, I., Borges, R.J., Gonzalez-Ramirez, A.M., Corzana, F., Clausen, H., Rovira, C., Hurtado-Guerrero, R.

	Description: Crystal structure of the AM0627 (E326A) inactive mutant in complex with PSGL-1-like bis-T glycopeptide and Zn2+		Description: Crystal structure of the AM0627 (E326A) inactive mutant in complex with PSGL-1-like bis-T glycopeptide and Zn2+
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Borges, R.J]]		[[Category: Borges, R.J]]
		+	[[Category: Gonzalez-Ramirez, A.M]]
	[[Category: Hurtado-Guerrero, R]]		[[Category: Hurtado-Guerrero, R]]
	[[Category: Taleb, V]]		[[Category: Taleb, V]]

OCA: Protected "7yx8" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2022 06:46:43 GMT

Protected "7yx8" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:46, 23 February 2022

OCA: New page: '''Unreleased structure''' The entry 7yx8 is ON HOLD Authors: Taleb, V., Liao, Q., Narimatsu, Y., Garcia-Garcia, A., Companon, I., Borges, R.J., Corzana, F., Clausen, H., Rovira, C., Hu...

OCA — Wed, 23 Feb 2022 06:46:42 GMT

New page: '''Unreleased structure''' The entry 7yx8 is ON HOLD Authors: Taleb, V., Liao, Q., Narimatsu, Y., Garcia-Garcia, A., Companon, I., Borges, R.J., Corzana, F., Clausen, H., Rovira, C., Hu...

New page

'''Unreleased structure'''

The entry 7yx8 is ON HOLD

Authors: Taleb, V., Liao, Q., Narimatsu, Y., Garcia-Garcia, A., Companon, I., Borges, R.J., Corzana, F., Clausen, H., Rovira, C., Hurtado-Guerrero, R.

Description: Crystal structure of the AM0627 (E326A) inactive mutant in complex with PSGL-1-like bis-T glycopeptide and Zn2+
[[Category: Unreleased Structures]]
[[Category: Borges, R.J]]
[[Category: Hurtado-Guerrero, R]]
[[Category: Taleb, V]]
[[Category: Clausen, H]]
[[Category: Narimatsu, Y]]
[[Category: Garcia-Garcia, A]]
[[Category: Corzana, F]]
[[Category: Companon, I]]
[[Category: Rovira, C]]
[[Category: Liao, Q]]