OCA at 09:00, 28 December 2022

OCA — Wed, 28 Dec 2022 09:00:03 GMT

←Older revision		Revision as of 09:00, 28 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7yyi is ~~ON HOLD until Paper~~ Publication	+	==Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) TMD==
		+	<StructureSection load='7yyi' size='340' side='right'caption='[[7yyi]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7yyi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YYI FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yyi OCA], [https://pdbe.org/7yyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yyi RCSB], [https://www.ebi.ac.uk/pdbsum/7yyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yyi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MGT5A_HUMAN MGT5A_HUMAN] Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Signal-Peptide Peptidase Like-3 (SPPL3) is an intramembrane cleaving aspartyl protease that causes secretion of extracellular domains from type-II transmembrane proteins. Numerous Golgi-localized glycosidases and glucosyltransferases have been identified as physiological SPPL3 substrates. By SPPL3 dependent processing, glycan-transferring enzymes are deactivated inside the cell, as their active site-containing domain is cleaved and secreted. Thus, SPPL3 impacts on glycan patterns of many cellular and secreted proteins and can regulate protein glycosylation. However, the characteristics that make a substrate a favourable candidate for SPPL3-dependent cleavage remain unknown. To gain insights into substrate requirements, we investigated the function of a GxxxG motif located in the transmembrane domain of N-acetylglucosaminyltransferase V (GnTV), a well-known SPPL3 substrate. SPPL3-dependent secretion of the substrate's ectodomain was affected by mutations disrupting the GxxxG motif. Using deuterium/hydrogen exchange and NMR spectroscopy, we studied the effect of these mutations on the helix flexibility of the GnTV transmembrane domain and observed that increased flexibility facilitates SPPL3-dependent shedding and vice versa. This study provides first insights into the characteristics of SPPL3 substrates, combining molecular biology, biochemistry, and biophysical techniques and its results will provide the basis for better understanding the characteristics of SPPL3 substrates with implications for the substrates of other intramembrane proteases.

-	~~Authors~~:	+	Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage.,Papadopoulou AA, Stelzer W, Silber M, Schlosser C, Spitz C, Haug-Kroper M, Straub T, Muller SA, Lichtenthaler SF, Muhle-Goll C, Langosch D, Fluhrer R Sci Rep. 2022 Dec 5;12(1):20987. doi: 10.1038/s41598-022-24772-8. PMID:36470941<ref>PMID:36470941</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7yyi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Muhle-Goll C]]
		+	[[Category: Silber M]]

OCA at 09:45, 30 March 2022

OCA — Wed, 30 Mar 2022 09:45:55 GMT

←Older revision		Revision as of 09:45, 30 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yyi is ON HOLD	+	The entry 7yyi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7yyi" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2022 06:48:45 GMT

Protected "7yyi" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:48, 23 February 2022

OCA: New page: '''Unreleased structure''' The entry 7yyi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 23 Feb 2022 06:48:44 GMT

New page: '''Unreleased structure''' The entry 7yyi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7yyi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7yyi - Revision history

OCA at 09:00, 28 December 2022

OCA at 09:45, 30 March 2022

OCA: Protected "7yyi" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7yyi is ON HOLD Authors: Description: Category: Unreleased Structures