7yzo - Revision history

OCA at 19:53, 7 September 2022

OCA — Wed, 07 Sep 2022 19:53:35 GMT

←Older revision		Revision as of 19:53, 7 September 2022
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	==Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA==		==Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA==
-	<StructureSection load='7yzo' size='340' side='right'caption='[[7yzo]]' scene=''>	+	<StructureSection load='7yzo' size='340' side='right'caption='[[7yzo]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7yzo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzo OCA], [https://pdbe.org/7yzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzo RCSB], [https://www.ebi.ac.uk/pdbsum/7yzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzo ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzo OCA], [https://pdbe.org/7yzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzo RCSB], [https://www.ebi.ac.uk/pdbsum/7yzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/SBCC_ECOLI SBCC_ECOLI]] SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA double-strand breaks (DSBs) threaten genome stability and are linked to tumorigenesis in humans. Repair of DSBs requires the removal of attached proteins and hairpins through a poorly understood but physiologically critical endonuclease activity by the Mre11-Rad50 complex. Here, we report cryoelectron microscopy (cryo-EM) structures of the bacterial Mre11-Rad50 homolog SbcCD bound to a protein-blocked DNA end and a DNA hairpin. The structures reveal that Mre11-Rad50 bends internal DNA for endonucleolytic cleavage and show how internal DNA, DNA ends, and hairpins are processed through a similar ATP-regulated conformational state. Furthermore, Mre11-Rad50 is loaded onto blocked DNA ends with Mre11 pointing away from the block, explaining the distinct biochemistries of 3' --> 5' exonucleolytic and endonucleolytic incision through the way Mre11-Rad50 interacts with diverse DNA ends. In summary, our results unify Mre11-Rad50's enigmatic nuclease diversity within a single structural framework and reveal how blocked DNA ends and hairpins are processed.
		+
		+	Structural mechanism of endonucleolytic processing of blocked DNA ends and hairpins by Mre11-Rad50.,Gut F, Kashammer L, Lammens K, Bartho JD, Boggusch AM, van de Logt E, Kessler B, Hopfner KP Mol Cell. 2022 Aug 12. pii: S1097-2765(22)00715-8. doi:, 10.1016/j.molcel.2022.07.019. PMID:35987200<ref>PMID:35987200</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7yzo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
	[[Category: Bartho J]]		[[Category: Bartho J]]
	[[Category: Gut F]]		[[Category: Gut F]]

OCA at 11:13, 31 August 2022

OCA — Wed, 31 Aug 2022 11:13:26 GMT

←Older revision		Revision as of 11:13, 31 August 2022
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-	====	+	==Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA==
	<StructureSection load='7yzo' size='340' side='right'caption='[[7yzo]]' scene=''>		<StructureSection load='7yzo' size='340' side='right'caption='[[7yzo]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzo OCA], [https://pdbe.org/7yzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzo RCSB], [https://www.ebi.ac.uk/pdbsum/7yzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzo ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzo OCA], [https://pdbe.org/7yzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzo RCSB], [https://www.ebi.ac.uk/pdbsum/7yzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzo ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bartho J]]
		+	[[Category: Gut F]]
		+	[[Category: Hopfner KP]]
		+	[[Category: Kaeshammer L]]
		+	[[Category: Kessler B]]
		+	[[Category: Lammens K]]
		+	[[Category: Van de Logt E]]

OCA at 06:24, 18 August 2022

OCA — Thu, 18 Aug 2022 06:24:19 GMT

←Older revision		Revision as of 06:24, 18 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7yzo ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7yzo' size='340' side='right'caption='[[7yzo]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzo OCA], [https://pdbe.org/7yzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzo RCSB], [https://www.ebi.ac.uk/pdbsum/7yzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:46, 30 March 2022

OCA — Wed, 30 Mar 2022 09:46:11 GMT

←Older revision		Revision as of 09:46, 30 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7yzo is ON HOLD	+	The entry 7yzo is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7yzo" [edit=sysop:move=sysop]

OCA — Wed, 02 Mar 2022 05:20:32 GMT

Protected "7yzo" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7yzo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Mar 2022 05:20:31 GMT

New page: '''Unreleased structure''' The entry 7yzo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7yzo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]