7z34 - Revision history

OCA at 06:19, 19 July 2023

OCA — Wed, 19 Jul 2023 06:19:15 GMT

←Older revision		Revision as of 06:19, 19 July 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7z34]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z34 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7z34]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z34 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z34 OCA], [https://pdbe.org/7z34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z34 RCSB], [https://www.ebi.ac.uk/pdbsum/7z34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z34 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z34 OCA], [https://pdbe.org/7z34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z34 RCSB], [https://www.ebi.ac.uk/pdbsum/7z34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z34 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AFG2_YEAST AFG2_YEAST]] ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (PubMed:12006565, PubMed:17646390, PubMed:23185031, PubMed:24371142). Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RLP24 from the pre-ribosomal particles (PubMed:17646390, PubMed:23185031, PubMed:24371142). This step facilitates the subsequent release of other shuttling proteins such as NOG1 and allows the transition of the pre-ribosomal particles to later maturation forms that bind REI1 (PubMed:17646390, PubMed:23185031, PubMed:24371142). Essential for viability (PubMed:8109176, PubMed:24371142).<ref>PMID:12006565</ref> <ref>PMID:17646390</ref> <ref>PMID:23185031</ref> <ref>PMID:24371142</ref> <ref>PMID:8109176</ref>	+	[https://www.uniprot.org/uniprot/AFG2_YEAST AFG2_YEAST] ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (PubMed:12006565, PubMed:17646390, PubMed:23185031, PubMed:24371142). Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RLP24 from the pre-ribosomal particles (PubMed:17646390, PubMed:23185031, PubMed:24371142). This step facilitates the subsequent release of other shuttling proteins such as NOG1 and allows the transition of the pre-ribosomal particles to later maturation forms that bind REI1 (PubMed:17646390, PubMed:23185031, PubMed:24371142). Essential for viability (PubMed:8109176, PubMed:24371142).<ref>PMID:12006565</ref> <ref>PMID:17646390</ref> <ref>PMID:23185031</ref> <ref>PMID:24371142</ref> <ref>PMID:8109176</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7z34" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7z34" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:43, 21 September 2022

OCA — Wed, 21 Sep 2022 07:43:39 GMT

←Older revision		Revision as of 07:43, 21 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7z34 is ~~ON HOLD~~	+	==Structure of pre-60S particle bound to DRG1(AFG2)==
		+	<StructureSection load='7z34' size='340' side='right'caption='[[7z34]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7z34]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z34 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z34 OCA], [https://pdbe.org/7z34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z34 RCSB], [https://www.ebi.ac.uk/pdbsum/7z34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z34 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/AFG2_YEAST AFG2_YEAST]] ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (PubMed:12006565, PubMed:17646390, PubMed:23185031, PubMed:24371142). Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RLP24 from the pre-ribosomal particles (PubMed:17646390, PubMed:23185031, PubMed:24371142). This step facilitates the subsequent release of other shuttling proteins such as NOG1 and allows the transition of the pre-ribosomal particles to later maturation forms that bind REI1 (PubMed:17646390, PubMed:23185031, PubMed:24371142). Essential for viability (PubMed:8109176, PubMed:24371142).<ref>PMID:12006565</ref> <ref>PMID:17646390</ref> <ref>PMID:23185031</ref> <ref>PMID:24371142</ref> <ref>PMID:8109176</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases.

-	~~Authors: Prattes~~, M., Grishkovskaya, I., ~~Bergler~~, H., ~~Haselbach~~, D.	+	Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.,Prattes M, Grishkovskaya I, Hodirnau VV, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H Nat Struct Mol Biol. 2022 Sep 12. pii: 10.1038/s41594-022-00832-5. doi:, 10.1038/s41594-022-00832-5. PMID:36097293<ref>PMID:36097293</ref>

-	~~Description: Structure~~ of ~~pre~~-~~60S particle bound to DRG1(AFG2)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bergler, H]]	+	<div class="pdbe-citations 7z34" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Prattes, M~~]]	+	== References ==
-	[[Category: Haselbach, D]]	+	<references/>
-	[[Category: ~~Grishkovskaya, I~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Bergler H]]
		+	[[Category: Grishkovskaya I]]
		+	[[Category: Haselbach D]]
		+	[[Category: Prattes M]]

OCA at 09:14, 18 May 2022

OCA — Wed, 18 May 2022 09:14:31 GMT

←Older revision		Revision as of 09:14, 18 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7z34 is ON HOLD ~~until sometime in the future~~	+	The entry 7z34 is ON HOLD

	Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D.		Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D.

OCA at 01:45, 21 April 2022

OCA — Thu, 21 Apr 2022 01:45:21 GMT

←Older revision		Revision as of 01:45, 21 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7z34 is ON HOLD	+	The entry 7z34 is ON HOLD until sometime in the future

	Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D.		Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D.
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	Description: Structure of pre-60S particle bound to DRG1(AFG2)		Description: Structure of pre-60S particle bound to DRG1(AFG2)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Grishkovskaya, I]]
	[[Category: Bergler, H]]		[[Category: Bergler, H]]
	[[Category: Prattes, M]]		[[Category: Prattes, M]]
	[[Category: Haselbach, D]]		[[Category: Haselbach, D]]
		+	[[Category: Grishkovskaya, I]]

OCA: Protected "7z34" [edit=sysop:move=sysop]

OCA — Wed, 09 Mar 2022 05:11:45 GMT

Protected "7z34" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 9 March 2022

OCA: New page: '''Unreleased structure''' The entry 7z34 is ON HOLD Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D. Description: Structure of pre-60S particle bound to DRG1(AFG2) ...

OCA — Wed, 09 Mar 2022 05:11:44 GMT

New page: '''Unreleased structure''' The entry 7z34 is ON HOLD Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D. Description: Structure of pre-60S particle bound to DRG1(AFG2) ...

New page

'''Unreleased structure'''

The entry 7z34 is ON HOLD

Authors: Prattes, M., Grishkovskaya, I., Bergler, H., Haselbach, D.

Description: Structure of pre-60S particle bound to DRG1(AFG2)
[[Category: Unreleased Structures]]
[[Category: Grishkovskaya, I]]
[[Category: Bergler, H]]
[[Category: Prattes, M]]
[[Category: Haselbach, D]]