7z7o - Revision history

OCA at 11:21, 15 November 2023

OCA — Wed, 15 Nov 2023 11:21:42 GMT

←Older revision		Revision as of 11:21, 15 November 2023
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	<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]], [[Resolution\|resolution]] 1.51Å' scene=''>		<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]], [[Resolution\|resolution]] 1.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7z7o]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z7o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=IO8:'>IO8</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.51Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IO8:2-[2-(aminomethyl)-4-(1H-indol-3-ylmethyl)-5-oxidanylidene-4H-imidazol-1-yl]ethanoic+acid'>IO8</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1S4NYF2_BRALA A0A1S4NYF2_BRALA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7z7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7z7o" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Branchiostoma lanceolatum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clavel, D]]	+	[[Category: Clavel D]]
-	[[Category: Dupuy, J]]	+	[[Category: Dupuy J]]
-	[[Category: Royant, A]]	+	[[Category: Royant A]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Mneongreen]]~~	+
-	~~[[Category: Rational design]]~~	+
-	~~[[Category: Trp-based fluorescent chromophore~~]]	+

OCA at 10:30, 18 May 2022

OCA — Wed, 18 May 2022 10:30:02 GMT

←Older revision		Revision as of 10:30, 18 May 2022
Line 1:		Line 1:

	==Structure of the fluorescent protein NeonCyan0.95 at pH 7.5==		==Structure of the fluorescent protein NeonCyan0.95 at pH 7.5==
-	<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]]' scene=''>	+	<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]], [[Resolution\|resolution]] 1.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7z7o]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IO8:'>IO8</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	mNeonGreen, an engineered green fluorescent protein (GFP) derived from lancelet, is one of the most brightly fluorescent homologues of Aequorea victoria jellyfish GFP (avGFP) yet reported. In this work, we investigated whether this bright fluorescence might be retained in homologs of mNeonGreen with modified chromophore structures and altered fluorescent hues. We found mNeonGreen to be generally less tolerant than avGFP to chromophore modification by substitution of the key chromophore-forming tyrosine residue with other aromatic amino acids. However, we were ultimately successful in creating a variant, designated as NeonCyan1, with a tryptophan-derived cyan fluorescent protein (CFP)-type chromophore, and two additional mutants with distinct spectral hues. Structural, computational, and photophysical characterization of NeonCyan1 and its variants provided insight into the factors that control the fluorescence emission color. Though not recommended as replacements for contemporary CFP variants, we demonstrate that NeonCyan1 variants are potentially suitable for live cell imaging applications.
		+
		+	Cyan fluorescent proteins derived from mNeonGreen.,Zarowny L, Clavel D, Johannson R, Duarte K, Depernet H, Dupuy J, Baker H, Brown A, Royant A, Campbell RE Protein Eng Des Sel. 2022 Apr 13. pii: 6567951. doi: 10.1093/protein/gzac004. PMID:35417013<ref>PMID:35417013</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7z7o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clavel D]]	+	[[Category: Clavel, D]]
-	[[Category: Dupuy J]]	+	[[Category: Dupuy, J]]
-	[[Category: Royant A]]	+	[[Category: Royant, A]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Mneongreen]]
		+	[[Category: Rational design]]
		+	[[Category: Trp-based fluorescent chromophore]]

OCA at 12:55, 4 May 2022

OCA — Wed, 04 May 2022 12:55:23 GMT

←Older revision		Revision as of 12:55, 4 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7z7o ~~is ON HOLD~~	+	==Structure of the fluorescent protein NeonCyan0.95 at pH 7.5==
-		+	<StructureSection load='7z7o' size='340' side='right'caption='[[7z7o]]' scene=''>
-	~~Authors~~: ~~Clavel~~, D., ~~Dupuy~~, J., ~~Royant, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z7O FirstGlance]. <br>
-	~~Description~~: ~~Structure of the fluorescent protein NeonCyan0~~.~~95 at pH 7~~.5	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z7o OCA], [https://pdbe.org/7z7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z7o RCSB], [https://www.ebi.ac.uk/pdbsum/7z7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z7o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Clavel, D]]	+	__TOC__
-	[[Category: ~~Royant, A~~]]	+	</StructureSection>
-	[[Category: ~~Dupuy, J~~]]	+	[[Category: Large Structures]]
		+	[[Category: Clavel D]]
		+	[[Category: Dupuy J]]
		+	[[Category: Royant A]]

OCA at 10:38, 27 April 2022

OCA — Wed, 27 Apr 2022 10:38:14 GMT

←Older revision		Revision as of 10:38, 27 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7z7o is ON HOLD ~~until sometime in the future~~	+	The entry 7z7o is ON HOLD

	Authors: Clavel, D., Dupuy, J., Royant, A.		Authors: Clavel, D., Dupuy, J., Royant, A.

OCA at 09:20, 6 April 2022

OCA — Wed, 06 Apr 2022 09:20:59 GMT

←Older revision		Revision as of 09:20, 6 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7z7o is ON HOLD	+	The entry 7z7o is ON HOLD until sometime in the future

	Authors: Clavel, D., Dupuy, J., Royant, A.		Authors: Clavel, D., Dupuy, J., Royant, A.

OCA: Protected "7z7o" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2022 10:10:30 GMT

Protected "7z7o" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:10, 23 March 2022

OCA: New page: '''Unreleased structure''' The entry 7z7o is ON HOLD Authors: Clavel, D., Dupuy, J., Royant, A. Description: Structure of the fluorescent protein NeonCyan0.95 at pH 7.5 [[Category: Unr...

OCA — Wed, 23 Mar 2022 10:10:29 GMT

New page: '''Unreleased structure''' The entry 7z7o is ON HOLD Authors: Clavel, D., Dupuy, J., Royant, A. Description: Structure of the fluorescent protein NeonCyan0.95 at pH 7.5 [[Category: Unr...

New page

'''Unreleased structure'''

The entry 7z7o is ON HOLD

Authors: Clavel, D., Dupuy, J., Royant, A.

Description: Structure of the fluorescent protein NeonCyan0.95 at pH 7.5
[[Category: Unreleased Structures]]
[[Category: Clavel, D]]
[[Category: Royant, A]]
[[Category: Dupuy, J]]