7zah - Revision history

OCA at 19:54, 7 September 2022

OCA — Wed, 07 Sep 2022 19:54:14 GMT

←Older revision		Revision as of 19:54, 7 September 2022
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-	====	+	==Cryo-EM structure of an archaeal aIF5B bound to the ssu translation initiation complex==
-	<StructureSection load='7zah' size='340' side='right'caption='[[7zah]]' scene=''>	+	<StructureSection load='7zah' size='340' side='right'caption='[[7zah]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7zah]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi_GE5 Pyrococcus abyssi GE5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZAH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZAH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zah OCA], [https://pdbe.org/7zah PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zah RCSB], [https://www.ebi.ac.uk/pdbsum/7zah PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zah ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4AC:N(4)-ACETYLCYTIDINE-5-MONOPHOSPHATE'>4AC</scene>, <scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=6MZ:N6-METHYLADENOSINE-5-MONOPHOSPHATE'>6MZ</scene>, <scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene>, <scene name='pdbligand=B8H:[(2~{R},3~{S},4~{R},5~{S})-5-[1-methyl-2,4-bis(oxidanylidene)pyrimidin-5-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+dihydrogen+phosphate'>B8H</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=LHH:[(2~{R},3~{R},4~{R},5~{R})-5-(4-acetamido-2-oxidanylidene-pyrimidin-1-yl)-4-methoxy-3-oxidanyl-oxolan-2-yl]methyl+dihydrogen+phosphate'>LHH</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zah OCA], [https://pdbe.org/7zah PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zah RCSB], [https://www.ebi.ac.uk/pdbsum/7zah PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zah ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/RS3A_PYRAB RS3A_PYRAB]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors e/aIF5B and e/aIF1A. In eukaryotes, the role of eIF5B in ribosomal subunit joining is established and structural data showing eIF5B bound to the full ribosome were obtained. To achieve its function, eIF5B collaborates with eIF1A. However, structural data illustrating how these two factors interact on the small ribosomal subunit have long been awaited. The role of the archaeal counterparts, aIF5B and aIF1A, remains to be extensively addressed. Here, we study the late steps of Pyrococcus abyssi translation initiation. Using in vitro reconstituted initiation complexes and light scattering, we show that aIF5B bound to GTP accelerates subunit joining without the need for GTP hydrolysis. We report the crystallographic structures of aIF5B bound to GDP and GTP and analyze domain movements associated to these two nucleotide states. Finally, we present the cryo-EM structure of an initiation complex containing 30S bound to mRNA, Met-tRNAiMet, aIF5B and aIF1A at 2.7 A resolution. Structural data shows how archaeal 5B and 1A factors cooperate to induce a conformation of the initiator tRNA favorable to subunit joining. Archaeal and eukaryotic features of late steps of translation initiation are discussed.
		+
		+	Role of aIF5B in archaeal translation initiation.,Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E Nucleic Acids Res. 2022 Jun 24;50(11):6532-6548. doi: 10.1093/nar/gkac490. PMID:35694843<ref>PMID:35694843</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7zah" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pyrococcus abyssi GE5]]
		+	[[Category: Bourgeois G]]
		+	[[Category: Coureux PD]]
		+	[[Category: Kazan R]]
		+	[[Category: Mechulam Y]]
		+	[[Category: Schmitt E]]

OCA at 04:55, 3 August 2022

OCA — Wed, 03 Aug 2022 04:55:23 GMT

←Older revision		Revision as of 04:55, 3 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zah ~~is ON HOLD until sometime in the future~~	+	====
-		+	<StructureSection load='7zah' size='340' side='right'caption='[[7zah]]' scene=''>
-	~~Authors~~: ~~Coureux, P~~.D.~~, Bourgeois, G~~.~~, Mechulam, Y~~.~~, Schmitt, E~~.~~, Kazan, R~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Cryo-EM structure of an archaeal aIF5B bound to the ssu translation initiation complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zah OCA], [https://pdbe.org/7zah PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zah RCSB], [https://www.ebi.ac.uk/pdbsum/7zah PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zah ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Kazan~~, ~~R]]~~	+	__TOC__
-	[~~[Category~~: ~~Bourgeois~~, G]]	+	</StructureSection>
-	[~~[Category~~: ~~Schmitt~~, E]]	+	[[Category: Large Structures]]
-	[[Category: ~~Coureux, P.D~~]]	+	[[Category: Z-disk]]
-	[[Category: ~~Mechulam, Y~~]]	+

OCA at 04:04, 13 July 2022

OCA — Wed, 13 Jul 2022 04:04:11 GMT

←Older revision		Revision as of 04:04, 13 July 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zah is ON HOLD	+	The entry 7zah is ON HOLD until sometime in the future

	Authors: Coureux, P.D., Bourgeois, G., Mechulam, Y., Schmitt, E., Kazan, R.		Authors: Coureux, P.D., Bourgeois, G., Mechulam, Y., Schmitt, E., Kazan, R.
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	Description: Cryo-EM structure of an archaeal aIF5B bound to the ssu translation initiation complex		Description: Cryo-EM structure of an archaeal aIF5B bound to the ssu translation initiation complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Schmitt, E]]
-	[[Category: Mechulam, Y]]
	[[Category: Kazan, R]]		[[Category: Kazan, R]]
	[[Category: Bourgeois, G]]		[[Category: Bourgeois, G]]
		+	[[Category: Schmitt, E]]
	[[Category: Coureux, P.D]]		[[Category: Coureux, P.D]]
		+	[[Category: Mechulam, Y]]

OCA: Protected "7zah" [edit=sysop:move=sysop]

OCA — Wed, 30 Mar 2022 09:50:25 GMT

Protected "7zah" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:50, 30 March 2022

OCA: New page: '''Unreleased structure''' The entry 7zah is ON HOLD Authors: Coureux, P.D., Bourgeois, G., Mechulam, Y., Schmitt, E., Kazan, R. Description: Cryo-EM structure of an archaeal aIF5B bou...

OCA — Wed, 30 Mar 2022 09:50:24 GMT

New page: '''Unreleased structure''' The entry 7zah is ON HOLD Authors: Coureux, P.D., Bourgeois, G., Mechulam, Y., Schmitt, E., Kazan, R. Description: Cryo-EM structure of an archaeal aIF5B bou...

New page

'''Unreleased structure'''

The entry 7zah is ON HOLD

Authors: Coureux, P.D., Bourgeois, G., Mechulam, Y., Schmitt, E., Kazan, R.

Description: Cryo-EM structure of an archaeal aIF5B bound to the ssu translation initiation complex
[[Category: Unreleased Structures]]
[[Category: Schmitt, E]]
[[Category: Mechulam, Y]]
[[Category: Kazan, R]]
[[Category: Bourgeois, G]]
[[Category: Coureux, P.D]]