OCA at 13:30, 1 February 2024

OCA — Thu, 01 Feb 2024 13:30:47 GMT

←Older revision		Revision as of 13:30, 1 February 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 7zb8 is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of SARS-CoV-2 Main Protease (Mpro) variant K61A at 2.48 A resolution==
-		+	<StructureSection load='7zb8' size='340' side='right'caption='[[7zb8]], [[Resolution\|resolution]] 2.48Å' scene=''>
-	~~Authors~~: ~~Paknia~~, E., ~~Rabe von Pappenheim~~, F., ~~Funk~~, L.-M., ~~Tittmann~~, K., ~~Chari~~, A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7zb8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZB8 FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure~~ of ~~SARS~~-~~CoV~~-2 ~~Main Protease~~ (~~Mpro~~) ~~variant K61A at~~ 2.~~48 A resolution~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.48Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>
-	[[Category: ~~Funk, L.-M~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zb8 OCA], [https://pdbe.org/7zb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zb8 RCSB], [https://www.ebi.ac.uk/pdbsum/7zb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zb8 ProSAT]</span></td></tr>
-	[[Category: ~~Tittmann, K~~]]	+	</table>
-	[[Category: ~~Chari, A~~]]	+	== Function ==
-	[[Category: Paknia, E]]	+	[https://www.uniprot.org/uniprot/R1AB_SARS2 R1AB_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN] (PubMed:32198291). Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7]<ref>PMID:32198291</ref> Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7] Responsible for replication and transcription of the viral RNA genome.[UniProtKB:P0C6X7] Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.[UniProtKB:P0C6X7] Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. Acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens.[UniProtKB:P0C6X7] Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.[UniProtKB:P0C6X7] Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.[UniProtKB:P0C6X7]
-	[[Category: Rabe ~~Von~~ Pappenheim, F]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Chari A]]
		+	[[Category: Funk L-M]]
		+	[[Category: Paknia E]]
		+	[[Category: Rabe von Pappenheim F]]
		+	[[Category: Tittmann K]]

OCA at 09:21, 6 April 2022

OCA — Wed, 06 Apr 2022 09:21:35 GMT

←Older revision		Revision as of 09:21, 6 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zb8 is ON HOLD	+	The entry 7zb8 is ON HOLD until Paper Publication

	Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A.		Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A.

OCA: Protected "7zb8" [edit=sysop:move=sysop]

OCA — Wed, 30 Mar 2022 09:51:36 GMT

Protected "7zb8" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:51, 30 March 2022

OCA: New page: '''Unreleased structure''' The entry 7zb8 is ON HOLD Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A. Description: Crystal Structure of SARS-CoV-2 Mai...

OCA — Wed, 30 Mar 2022 09:51:35 GMT

New page: '''Unreleased structure''' The entry 7zb8 is ON HOLD Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A. Description: Crystal Structure of SARS-CoV-2 Mai...

New page

'''Unreleased structure'''

The entry 7zb8 is ON HOLD

Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A.

Description: Crystal Structure of SARS-CoV-2 Main Protease (Mpro) variant K61A at 2.48 A resolution
[[Category: Unreleased Structures]]
[[Category: Funk, L.-M]]
[[Category: Tittmann, K]]
[[Category: Chari, A]]
[[Category: Paknia, E]]
[[Category: Rabe Von Pappenheim, F]]

7zb8 - Revision history

OCA at 13:30, 1 February 2024

OCA at 09:21, 6 April 2022

OCA: Protected "7zb8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7zb8 is ON HOLD Authors: Paknia, E., Rabe von Pappenheim, F., Funk, L.-M., Tittmann, K., Chari, A. Description: Crystal Structure of SARS-CoV-2 Mai...