OCA at 13:30, 1 February 2024

OCA — Thu, 01 Feb 2024 13:30:58 GMT

←Older revision		Revision as of 13:30, 1 February 2024
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-	====	+	==Unspecific peroxygenase from Collariella virescens==
-	<StructureSection load='7zcl' size='340' side='right'caption='[[7zcl]]' scene=''>	+	<StructureSection load='7zcl' size='340' side='right'caption='[[7zcl]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7zcl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Collariella_virescens Collariella virescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZCL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zcl OCA], [https://pdbe.org/7zcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zcl RCSB], [https://www.ebi.ac.uk/pdbsum/7zcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zcl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zcl OCA], [https://pdbe.org/7zcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zcl RCSB], [https://www.ebi.ac.uk/pdbsum/7zcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zcl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unspecific peroxygenases (UPOs) are extracellular fungal enzymes of biotechnological interest as self-sufficient (and more stable) counterparts of cytochrome P450 monooxygenases, the latter being present in most living cells. Expression hosts and structural information are crucial for exploiting UPO diversity (over eight thousand UPO-type genes were identified in sequenced genomes) in target reactions of industrial interest. However, while many thousands of entries in the Protein Data Bank include molecular coordinates of P450 enzymes, only 19 entries correspond to UPO enzymes, and UPO structures from only two species (Agrocybe aegerita and Hypoxylon sp.) have been published to date. In the present study, two UPOs from the basidiomycete Marasmius rotula (rMroUPO) and the ascomycete Collariella virescens (rCviUPO) were crystallized after sequence optimization and Escherichia coli expression as active soluble enzymes. Crystals of rMroUPO and rCviUPO were obtained at sufficiently high resolution (1.45 and 1.95 A, respectively) and the corresponding structures were solved by molecular replacement. The crystal structures of the two enzymes (and two mutated variants) showed dimeric proteins. Complementary biophysical and molecular biology studies unveiled the diverse structural bases of the dimeric nature of the two enzymes. Intermolecular disulfide bridge and parallel association between two alpha-helices, among other interactions, were identified at the dimer interfaces. Interestingly, one of the rCviUPO variants incorporated the ability to produce fatty acid diepoxides-reactive compounds with valuable cross-linking capabilities-due to removal of the enzyme C-terminal tail located near the entrance of the heme access channel. In conclusion, different dimeric arrangements could be described in (short) UPO crystal structures.
		+
		+	Structural Characterization of Two Short Unspecific Peroxygenases: Two Different Dimeric Arrangements.,Linde D, Santillana E, Fernandez-Fueyo E, Gonzalez-Benjumea A, Carro J, Gutierrez A, Martinez AT, Romero A Antioxidants (Basel). 2022 Apr 30;11(5). pii: antiox11050891. doi:, 10.3390/antiox11050891. PMID:35624755<ref>PMID:35624755</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7zcl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Collariella virescens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Romero A]]
		+	[[Category: Santillana E]]

OCA at 10:18, 18 May 2022

OCA — Wed, 18 May 2022 10:18:48 GMT

←Older revision		Revision as of 10:18, 18 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zcl ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7zcl' size='340' side='right'caption='[[7zcl]]' scene=''>
-	~~Authors~~: ~~Santillana, E~~.~~, Romero, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Unspecific peroxygenase from Collariella virescens~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zcl OCA], [https://pdbe.org/7zcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zcl RCSB], [https://www.ebi.ac.uk/pdbsum/7zcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zcl ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Santillana, E~~]]	+	__TOC__
-	[[Category: ~~Romero, A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:46, 13 April 2022

OCA — Wed, 13 Apr 2022 08:46:13 GMT

←Older revision

Revision as of 08:46, 13 April 2022

OCA: Protected "7zcl" [edit=sysop:move=sysop]

OCA — Wed, 06 Apr 2022 09:22:44 GMT

Protected "7zcl" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:22, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7zcl is ON HOLD Authors: Santillana, E., Romero, A. Description: Unspecific peroxygenase from Collariella virescens [[Category: Unreleased Structur...

OCA — Wed, 06 Apr 2022 09:22:43 GMT

New page: '''Unreleased structure''' The entry 7zcl is ON HOLD Authors: Santillana, E., Romero, A. Description: Unspecific peroxygenase from Collariella virescens [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 7zcl is ON HOLD

Authors: Santillana, E., Romero, A.

Description: Unspecific peroxygenase from Collariella virescens
[[Category: Unreleased Structures]]
[[Category: Romero, A]]
[[Category: Santillana, E]]

7zcl - Revision history

OCA at 13:30, 1 February 2024

OCA at 10:18, 18 May 2022

OCA at 08:46, 13 April 2022

OCA: Protected "7zcl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7zcl is ON HOLD Authors: Santillana, E., Romero, A. Description: Unspecific peroxygenase from Collariella virescens [[Category: Unreleased Structur...