7zp0 - Revision history

OCA at 08:08, 10 April 2024

OCA — Wed, 10 Apr 2024 08:08:35 GMT

←Older revision		Revision as of 08:08, 10 April 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A2J1D710_ECOLX A0A2J1D710_ECOLX] Member of a two-component regulatory system.[RuleBase:RU364088]		[https://www.uniprot.org/uniprot/A0A2J1D710_ECOLX A0A2J1D710_ECOLX] Member of a two-component regulatory system.[RuleBase:RU364088]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The CusS histidine kinase is a member of Escherichia coli two-component signal transduction system, engaged in a response to copper ions excess in the cell periplasm. The periplasmic sensor domain of CusS binds the free copper ions and the CusS kinase core phosphorylates the cognate CusR which regulates transcription of the efflux pomp CusCBA. A small amount of copper ions is indispensable for the aerobic cell metabolism. Nonetheless, its excess in the cytoplasm generates damaging and reactive hydroxyl radicals. For that reason, understanding the bacterial copper sensing mechanisms can contribute to reducing bacterial copper-resistance and developing bactericidal copper-based materials. The crystal structure of the CusS kinase core was solved at the resolution of 1.4 A. The cytoplasmic catalytic core domains formed a homodimer. Based on the obtained structure, the intramolecular and intermolecular interactions crucial for the mechanism of CusS autophosphorylation were described.
-
-	Crystal structure of the Escherichia coli CusS kinase core.,Cociurovscaia A, Bujacz G, Pietrzyk-Brzezinska AJ J Struct Biol. 2022 Jul 27;214(3):107883. doi: 10.1016/j.jsb.2022.107883. PMID:35907487<ref>PMID:35907487</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7zp0" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:33, 1 February 2024

OCA — Thu, 01 Feb 2024 13:33:39 GMT

←Older revision		Revision as of 13:33, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7zp0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_'BL21-Gold(DE3)pLysS_AG' Escherichia coli 'BL21-Gold(DE3)pLysS AG']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZP0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7zp0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_'BL21-Gold(DE3)pLysS_AG' Escherichia coli 'BL21-Gold(DE3)pLysS AG']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZP0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.398Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zp0 OCA], [https://pdbe.org/7zp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zp0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zp0 OCA], [https://pdbe.org/7zp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zp0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A2J1D710_ECOLX A0A2J1D710_ECOLX]] Member of a two-component regulatory system.[RuleBase:RU364088]	+	[https://www.uniprot.org/uniprot/A0A2J1D710_ECOLX A0A2J1D710_ECOLX] Member of a two-component regulatory system.[RuleBase:RU364088]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:36, 7 September 2022

OCA — Wed, 07 Sep 2022 19:36:26 GMT

←Older revision		Revision as of 19:36, 7 September 2022
Line 1:		Line 1:

	==Crystal structure of CusS histidine kinase catalytic core from Escherichia coli==		==Crystal structure of CusS histidine kinase catalytic core from Escherichia coli==
-	<StructureSection load='7zp0' size='340' side='right'caption='[[7zp0]]' scene=''>	+	<StructureSection load='7zp0' size='340' side='right'caption='[[7zp0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZP0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7zp0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_'BL21-Gold(DE3)pLysS_AG' Escherichia coli 'BL21-Gold(DE3)pLysS AG']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZP0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zp0 OCA], [https://pdbe.org/7zp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zp0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zp0 OCA], [https://pdbe.org/7zp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zp0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A2J1D710_ECOLX A0A2J1D710_ECOLX]] Member of a two-component regulatory system.[RuleBase:RU364088]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The CusS histidine kinase is a member of Escherichia coli two-component signal transduction system, engaged in a response to copper ions excess in the cell periplasm. The periplasmic sensor domain of CusS binds the free copper ions and the CusS kinase core phosphorylates the cognate CusR which regulates transcription of the efflux pomp CusCBA. A small amount of copper ions is indispensable for the aerobic cell metabolism. Nonetheless, its excess in the cytoplasm generates damaging and reactive hydroxyl radicals. For that reason, understanding the bacterial copper sensing mechanisms can contribute to reducing bacterial copper-resistance and developing bactericidal copper-based materials. The crystal structure of the CusS kinase core was solved at the resolution of 1.4 A. The cytoplasmic catalytic core domains formed a homodimer. Based on the obtained structure, the intramolecular and intermolecular interactions crucial for the mechanism of CusS autophosphorylation were described.
		+
		+	Crystal structure of the Escherichia coli CusS kinase core.,Cociurovscaia A, Bujacz G, Pietrzyk-Brzezinska AJ J Struct Biol. 2022 Jul 27;214(3):107883. doi: 10.1016/j.jsb.2022.107883. PMID:35907487<ref>PMID:35907487</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7zp0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:39, 31 August 2022

OCA — Wed, 31 Aug 2022 06:39:39 GMT

←Older revision		Revision as of 06:39, 31 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7zp0 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of CusS histidine kinase catalytic core from Escherichia coli==
-		+	<StructureSection load='7zp0' size='340' side='right'caption='[[7zp0]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZP0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zp0 OCA], [https://pdbe.org/7zp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zp0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bujacz G]]
		+	[[Category: Cociurovscaia A]]
		+	[[Category: Pietrzyk-Brzezinka A]]

OCA at 09:17, 18 May 2022

OCA — Wed, 18 May 2022 09:17:18 GMT

←Older revision		Revision as of 09:17, 18 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zp0 is ON HOLD	+	The entry 7zp0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7zp0" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2022 11:49:10 GMT

Protected "7zp0" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:49, 4 May 2022

OCA: New page: '''Unreleased structure''' The entry 7zp0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 May 2022 11:49:09 GMT

New page: '''Unreleased structure''' The entry 7zp0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7zp0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]