7zr2 - Revision history

OCA at 13:33, 1 February 2024

OCA — Thu, 01 Feb 2024 13:33:55 GMT

←Older revision		Revision as of 13:33, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7zr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7zr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZR2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zr2 OCA], [https://pdbe.org/7zr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zr2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zr2 OCA], [https://pdbe.org/7zr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zr2 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 7zr2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7zr2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Spike protein 3D structures\|Spike protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:29, 9 November 2022

OCA — Wed, 09 Nov 2022 07:29:33 GMT

←Older revision		Revision as of 07:29, 9 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7zr2 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of a chimeric protein mimic of SARS-CoV-2 Spike HR1 in complex with HR2==
		+	<StructureSection load='7zr2' size='340' side='right'caption='[[7zr2]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7zr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZR2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zr2 OCA], [https://pdbe.org/7zr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7zr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zr2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SARS-CoV-2 spike (S) protein mediates virus attachment to the cells and fusion between viral and cell membranes. Membrane fusion is driven by mutual interaction between the highly conserved heptad-repeat regions 1 and 2 (HR1 and HR2) of the S2 subunit of the spike. For this reason, these S2 regions are interesting therapeutic targets for COVID-19. Although HR1 and HR2 have been described as transiently exposed during the fusion process, no significant antibody responses against these S2 regions have been reported. Here we designed chimeric proteins that imitate highly stable HR1 helical trimers and strongly bind to HR2. The proteins have broad inhibitory activity against WT B.1 and BA.1 viruses. Sera from COVID-19 convalescent donors showed significant levels of reactive antibodies (IgG and IgA) against the HR1 mimetic proteins, whereas these antibody responses were absent in sera from uninfected donors. Moreover, both inhibitory activity and antigenicity of the proteins correlate positively with their structural stability but not with the number of amino acid changes in their HR1 sequences, indicating a conformational and conserved nature of the involved epitopes. Our results reveal previously undetected spike epitopes that may guide the design of new robust COVID-19 vaccines and therapies.

-	~~Authors: Camara~~-~~Artigas, A., Gavira, J.A~~., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F.	+	Novel chimeric proteins mimicking SARS-CoV-2 spike epitopes with broad inhibitory activity.,Cano-Munoz M, Polo-Megias D, Camara-Artigas A, Gavira JA, Lopez-Rodriguez MJ, Laumond G, Schmidt S, Demiselle J, Bahram S, Moog C, Conejero-Lara F Int J Biol Macromol. 2022 Oct 8. pii: S0141-8130(22)02263-2. doi:, 10.1016/j.ijbiomac.2022.10.031. PMID:36220405<ref>PMID:36220405</ref>

-	~~Description: Crystal structure of~~ a ~~chimeric protein mimic~~ of ~~SARS~~-~~CoV~~-~~2 Spike HR1 in complex with HR2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Polo-Megias, D~~]]	+	<div class="pdbe-citations 7zr2" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Gavira, J.~~A]]	+	== References ==
-	[[Category: Conejero-Lara, F]]	+	<references/>
-	[[Category: ~~Camara-Artigas, A~~]]	+	__TOC__
-	[[Category: ~~Cano~~-~~Munoz, M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Camara-Artigas A]]
		+	[[Category: Cano-Munoz M]]
		+	[[Category: Conejero-Lara F]]
		+	[[Category: Gavira JA]]
		+	[[Category: Polo-Megias D]]

OCA at 06:52, 25 May 2022

OCA — Wed, 25 May 2022 06:52:44 GMT

←Older revision		Revision as of 06:52, 25 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zr2 is ON HOLD	+	The entry 7zr2 is ON HOLD until Paper Publication

	Authors: Camara-Artigas, A., Gavira, J.A., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F.		Authors: Camara-Artigas, A., Gavira, J.A., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Polo-Megias, D]]		[[Category: Polo-Megias, D]]
-	[[Category: Camara-Artigas, A]]
	[[Category: Gavira, J.A]]		[[Category: Gavira, J.A]]
-	[[Category: Cano-Munoz, M]]
	[[Category: Conejero-Lara, F]]		[[Category: Conejero-Lara, F]]
		+	[[Category: Camara-Artigas, A]]
		+	[[Category: Cano-Munoz, M]]

OCA: Protected "7zr2" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 05:08:47 GMT

Protected "7zr2" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7zr2 is ON HOLD Authors: Camara-Artigas, A., Gavira, J.A., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F. Description: Crystal structure of a c...

OCA — Thu, 12 May 2022 05:08:46 GMT

New page: '''Unreleased structure''' The entry 7zr2 is ON HOLD Authors: Camara-Artigas, A., Gavira, J.A., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F. Description: Crystal structure of a c...

New page

'''Unreleased structure'''

The entry 7zr2 is ON HOLD

Authors: Camara-Artigas, A., Gavira, J.A., Cano-Munoz, M., Polo-Megias, D., Conejero-Lara, F.

Description: Crystal structure of a chimeric protein mimic of SARS-CoV-2 Spike HR1 in complex with HR2
[[Category: Unreleased Structures]]
[[Category: Polo-Megias, D]]
[[Category: Camara-Artigas, A]]
[[Category: Gavira, J.A]]
[[Category: Cano-Munoz, M]]
[[Category: Conejero-Lara, F]]