OCA at 20:10, 16 November 2022

OCA — Wed, 16 Nov 2022 20:10:35 GMT

←Older revision		Revision as of 20:10, 16 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zrd is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM map of the WT KdpFABC complex in the E1-P tight conformation, stabilised with the inhibitor orthovanadate==
		+	<StructureSection load='7zrd' size='340' side='right'caption='[[7zrd]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7zrd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZRD FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zrd OCA], [https://pdbe.org/7zrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zrd RCSB], [https://www.ebi.ac.uk/pdbsum/7zrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zrd ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KDPA_ECOLI KDPA_ECOLI] Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:2849541, PubMed:8499455, PubMed:23930894). This subunit binds and transports the potassium across the cytoplasmic membrane (PubMed:7896809).<ref>PMID:23930894</ref> <ref>PMID:2849541</ref> <ref>PMID:7896809</ref> <ref>PMID:8499455</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	KdpFABC is a high-affinity prokaryotic K<sup>+</sup> uptake system that forms a functional chimera between a channel-like subunit (KdpA) and a P-type ATPase (KdpB). At high K<sup>+</sup> levels, KdpFABC needs to be inhibited to prevent excessive K<sup>+</sup> accumulation to the point of toxicity. This is achieved by a phosphorylation of the serine residue in the TGES<sub>162</sub> motif in the A domain of the pump subunit KdpB (KdpB<sub>S162-P</sub>). Here, we explore the structural basis of inhibition by KdpB<sub>S162</sub> phosphorylation by determining the conformational landscape of KdpFABC under inhibiting and non-inhibiting conditions. Under turnover conditions, we identified a new inhibited KdpFABC state that we termed E1P tight, which is not part of the canonical Post-Albers transport cycle of P-type ATPases. It likely represents the biochemically described stalled E1P state adopted by KdpFABC upon KdpB<sub>S162</sub> phosphorylation. The E1P tight state exhibits a compact fold of the three cytoplasmic domains and is likely adopted when the transition from high-energy E1P states to E2P states is unsuccessful. This study represents a structural characterization of a biologically relevant off-cycle state in the P-type ATPase family and supports the emerging discussion of P-type ATPase regulation by such states.

-	~~Authors~~:	+	Inhibited KdpFABC transitions into an E1 off-cycle state.,Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C Elife. 2022 Oct 18;11. pii: 80988. doi: 10.7554/eLife.80988. PMID:36255052<ref>PMID:36255052</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7zrd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Corey RA]]
		+	[[Category: Haenelt I]]
		+	[[Category: Hielkema L]]
		+	[[Category: Paulino C]]
		+	[[Category: Silberberg JM]]
		+	[[Category: Stansfeld PJ]]
		+	[[Category: Stock C]]
		+	[[Category: Wunnicke D]]

OCA at 04:13, 8 June 2022

OCA — Wed, 08 Jun 2022 04:13:09 GMT

←Older revision		Revision as of 04:13, 8 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zrd is ON HOLD	+	The entry 7zrd is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7zrd" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 05:09:23 GMT

Protected "7zrd" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:09, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7zrd is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 12 May 2022 05:09:22 GMT

New page: '''Unreleased structure''' The entry 7zrd is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7zrd is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7zrd - Revision history

OCA at 20:10, 16 November 2022

OCA at 04:13, 8 June 2022

OCA: Protected "7zrd" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7zrd is ON HOLD Authors: Description: Category: Unreleased Structures