7zrq - Revision history

OCA at 13:34, 1 February 2024

OCA — Thu, 01 Feb 2024 13:34:02 GMT

←Older revision		Revision as of 13:34, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7zrq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZRQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[7zrq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZRQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.68Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zrq OCA], [https://pdbe.org/7zrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zrq RCSB], [https://www.ebi.ac.uk/pdbsum/7zrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zrq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zrq OCA], [https://pdbe.org/7zrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zrq RCSB], [https://www.ebi.ac.uk/pdbsum/7zrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zrq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:02, 28 December 2022

OCA — Wed, 28 Dec 2022 09:02:42 GMT

←Older revision		Revision as of 09:02, 28 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7zrq is ~~ON HOLD~~ ~~until 00 0001~~	+	==1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIIdelta peptide complex==
		+	<StructureSection load='7zrq' size='340' side='right'caption='[[7zrq]], [[Resolution\|resolution]] 1.68Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7zrq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZRQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zrq OCA], [https://pdbe.org/7zrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zrq RCSB], [https://www.ebi.ac.uk/pdbsum/7zrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zrq ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Long QT syndrome (LQTS) is a human inherited heart condition that can cause life-threatening arrhythmia including sudden cardiac death. Mutations in the ubiquitous Ca(2+)-sensing protein calmodulin (CaM) are associated with LQTS, but the molecular mechanism by which these mutations lead to irregular heartbeats is not fully understood. Here, we use a multidisciplinary approach including protein biophysics, structural biology, confocal imaging and patch-clamp electrophysiology to determine the effect of the disease-associated CaM mutation E140G on CaM structure and function. We present novel data showing that mutant-regulated CaMKIIdelta kinase activity is impaired with a significant reduction in enzyme autophosphorylation rate. We report the first high-resolution crystal structure of a LQTS-associated CaM variant in complex with the CaMKIIdelta peptide, which shows significant structural differences, compared to the wild-type complex. Furthermore, we demonstrate that the E140G mutation significantly disrupted Ca(v)1.2 Ca(2+)/CaM-dependent inactivation, while cardiac ryanodine receptor (RyR2) activity remained unaffected. In addition, we show that the LQTS-associated mutation alters CaM's Ca(2+) binding characteristics, secondary structure content and interaction with key partners involved in excitation-contraction coupling (CaMKIIdelta, Ca(v)1.2, RyR2). In conclusion, LQTS-associated CaM mutation E140G severely impacts the structure-function relationship of CaM and its regulation of CaMKIIdelta and Ca(v)1.2. This provides a crucial insight into the molecular factors contributing to CaM-mediated arrhythmias with a central role for CaMKIIdelta.

-	~~Authors: Helassa~~, N., ~~Antonyuk~~, S.	+	Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIdelta autophosphorylation and L-type calcium channel (Ca(v)1.2) inactivation.,Prakash O, Gupta N, Milburn A, McCormick L, Deugi V, Fisch P, Wyles J, Thomas NL, Antonyuk S, Dart C, Helassa N J Biol Chem. 2022 Dec 7:102777. doi: 10.1016/j.jbc.2022.102777. PMID:36496072<ref>PMID:36496072</ref>

-	~~Description: 1~~.~~68 Angstrom crystal structure~~ of Ca/~~CaM~~-~~E140G~~:~~CaMKIIdelta peptide complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Helassa, N~~]]	+	<div class="pdbe-citations 7zrq" style="background-color:#fffaf0;"></div>
-	[[Category: Antonyuk, S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Antonyuk S]]
		+	[[Category: Helassa N]]

OCA at 04:04, 13 July 2022

OCA — Wed, 13 Jul 2022 04:04:43 GMT

←Older revision		Revision as of 04:04, 13 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7zrq is ON HOLD	+	The entry 7zrq is ON HOLD until 00 0001

	Authors: Helassa, N., Antonyuk, S.		Authors: Helassa, N., Antonyuk, S.

OCA at 07:13, 22 June 2022

OCA — Wed, 22 Jun 2022 07:13:56 GMT

←Older revision		Revision as of 07:13, 22 June 2022
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	Authors: Helassa, N., Antonyuk, S.		Authors: Helassa, N., Antonyuk, S.

-	Description: 1.68 Angstrom crystal structure of Ca/CaM-E140G:~~CaMKIId~~ peptide complex	+	Description: 1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIIdelta peptide complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Helassa, N]]		[[Category: Helassa, N]]
	[[Category: Antonyuk, S]]		[[Category: Antonyuk, S]]

OCA: Protected "7zrq" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 05:10:04 GMT

Protected "7zrq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:10, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7zrq is ON HOLD Authors: Helassa, N., Antonyuk, S. Description: 1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIId peptide complex [[Category:...

OCA — Thu, 12 May 2022 05:10:02 GMT

New page: '''Unreleased structure''' The entry 7zrq is ON HOLD Authors: Helassa, N., Antonyuk, S. Description: 1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIId peptide complex [[Category:...

New page

'''Unreleased structure'''

The entry 7zrq is ON HOLD

Authors: Helassa, N., Antonyuk, S.

Description: 1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIId peptide complex
[[Category: Unreleased Structures]]
[[Category: Helassa, N]]
[[Category: Antonyuk, S]]