8a0j - Revision history

OCA at 07:55, 1 May 2024

OCA — Wed, 01 May 2024 07:55:53 GMT

←Older revision		Revision as of 07:55, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8a0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense_IL3000 Trypanosoma congolense IL3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0J FirstGlance]. <br>		<table><tr><td colspan='2'>[[8a0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense_IL3000 Trypanosoma congolense IL3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0j OCA], [https://pdbe.org/8a0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0j RCSB], [https://www.ebi.ac.uk/pdbsum/8a0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0j ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0j OCA], [https://pdbe.org/8a0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0j RCSB], [https://www.ebi.ac.uk/pdbsum/8a0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/G0V1V5_TRYCI G0V1V5_TRYCI]		[https://www.uniprot.org/uniprot/G0V1V5_TRYCI G0V1V5_TRYCI]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Chromosome segregation requires assembly of the macromolecular kinetochore complex onto centromeric DNA. While most eukaryotes have canonical kinetochore proteins that are widely conserved among eukaryotes, evolutionarily divergent kinetoplastids have a unique set of kinetochore proteins. Little is known about the mechanism of kinetochore assembly in kinetoplastids. Here we characterize two homologous kinetoplastid kinetochore proteins, KKT2 and KKT3, that constitutively localize at centromeres. They have three domains that are highly conserved among kinetoplastids: an N-terminal kinase domain of unknown function, the centromere localization domain in the middle, and the C-terminal domain that has weak similarity to polo boxes of Polo-like kinases. We show that the kinase activity of KKT2 is essential for accurate chromosome segregation, while that of KKT3 is dispensable for cell growth in Trypanosoma brucei. Crystal structures of their divergent polo boxes reveal differences between KKT2 and KKT3. We also show that the divergent polo boxes of KKT3 are sufficient to recruit KKT2 in trypanosomes. Furthermore, we demonstrate that the divergent polo boxes of KKT2 directly interact with KKT1 and that KKT1 interacts with KKT6. These results show that the divergent polo boxes of KKT2 and KKT3 are protein-protein interaction domains, which initiate kinetochore assembly in T. brucei.
-
-	Divergent polo boxes in KKT2 bind KKT1 to initiate the kinetochore assembly cascade in Trypanosoma brucei.,Ishii M, Ludzia P, Marciano G, Allen W, Nerusheva OO, Akiyoshi B Mol Biol Cell. 2022 Sep 21:mbcE22070269T. doi: 10.1091/mbc.E22-07-0269-T. PMID:36129769<ref>PMID:36129769</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8a0j" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:06, 30 November 2022

OCA — Wed, 30 Nov 2022 11:06:22 GMT

←Older revision		Revision as of 11:06, 30 November 2022
Line 1:		Line 1:

	==Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain==		==Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain==
-	<StructureSection load='8a0j' size='340' side='right'caption='[[8a0j]]' scene=''>	+	<StructureSection load='8a0j' size='340' side='right'caption='[[8a0j]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8a0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense_IL3000 Trypanosoma congolense IL3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0J FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0j OCA], [https://pdbe.org/8a0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0j RCSB], [https://www.ebi.ac.uk/pdbsum/8a0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0j ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0j OCA], [https://pdbe.org/8a0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0j RCSB], [https://www.ebi.ac.uk/pdbsum/8a0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0V1V5_TRYCI G0V1V5_TRYCI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chromosome segregation requires assembly of the macromolecular kinetochore complex onto centromeric DNA. While most eukaryotes have canonical kinetochore proteins that are widely conserved among eukaryotes, evolutionarily divergent kinetoplastids have a unique set of kinetochore proteins. Little is known about the mechanism of kinetochore assembly in kinetoplastids. Here we characterize two homologous kinetoplastid kinetochore proteins, KKT2 and KKT3, that constitutively localize at centromeres. They have three domains that are highly conserved among kinetoplastids: an N-terminal kinase domain of unknown function, the centromere localization domain in the middle, and the C-terminal domain that has weak similarity to polo boxes of Polo-like kinases. We show that the kinase activity of KKT2 is essential for accurate chromosome segregation, while that of KKT3 is dispensable for cell growth in Trypanosoma brucei. Crystal structures of their divergent polo boxes reveal differences between KKT2 and KKT3. We also show that the divergent polo boxes of KKT3 are sufficient to recruit KKT2 in trypanosomes. Furthermore, we demonstrate that the divergent polo boxes of KKT2 directly interact with KKT1 and that KKT1 interacts with KKT6. These results show that the divergent polo boxes of KKT2 and KKT3 are protein-protein interaction domains, which initiate kinetochore assembly in T. brucei.
		+
		+	Divergent polo boxes in KKT2 bind KKT1 to initiate the kinetochore assembly cascade in Trypanosoma brucei.,Ishii M, Ludzia P, Marciano G, Allen W, Nerusheva OO, Akiyoshi B Mol Biol Cell. 2022 Sep 21:mbcE22070269T. doi: 10.1091/mbc.E22-07-0269-T. PMID:36129769<ref>PMID:36129769</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8a0j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Trypanosoma congolense IL3000]]
	[[Category: Akiyoshi B]]		[[Category: Akiyoshi B]]
	[[Category: Allen W]]		[[Category: Allen W]]

OCA at 05:13, 15 June 2022

OCA — Wed, 15 Jun 2022 05:13:15 GMT

←Older revision		Revision as of 05:13, 15 June 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 8a0j is ON HOLD~~	+	==Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain==
-		+	<StructureSection load='8a0j' size='340' side='right'caption='[[8a0j]]' scene=''>
-	~~Authors: Ishii, M., Ludzia, P., Marciano, G., Allen, W., Nerusheva, O., Akiyoshi, B.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A0J FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a0j OCA], [https://pdbe.org/8a0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a0j RCSB], [https://www.ebi.ac.uk/pdbsum/8a0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a0j ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Allen~~, W]]	+	__TOC__
-	[[Category: ~~Ludzia, P~~]]	+	</StructureSection>
-	[[Category: Akiyoshi, B]]	+	[[Category: Large Structures]]
-	[[Category: ~~Marciano, G~~]]	+	[[Category: Akiyoshi B]]
-	[[Category: Ishii, M]]	+	[[Category: Allen W]]
-	[[Category: Nerusheva, O]]	+	[[Category: Ishii M]]
		+	[[Category: Ludzia P]]
		+	[[Category: Marciano G]]
		+	[[Category: Nerusheva O]]

OCA: Protected "8a0j" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2022 04:21:27 GMT

Protected "8a0j" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:21, 8 June 2022

OCA: New page: '''Unreleased structure''' The entry 8a0j is ON HOLD Authors: Ishii, M., Ludzia, P., Marciano, G., Allen, W., Nerusheva, O., Akiyoshi, B. Description: Crystal structure of the kinetopl...

OCA — Wed, 08 Jun 2022 04:21:26 GMT

New page: '''Unreleased structure''' The entry 8a0j is ON HOLD Authors: Ishii, M., Ludzia, P., Marciano, G., Allen, W., Nerusheva, O., Akiyoshi, B. Description: Crystal structure of the kinetopl...

New page

'''Unreleased structure'''

The entry 8a0j is ON HOLD

Authors: Ishii, M., Ludzia, P., Marciano, G., Allen, W., Nerusheva, O., Akiyoshi, B.

Description: Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain
[[Category: Unreleased Structures]]
[[Category: Allen, W]]
[[Category: Ludzia, P]]
[[Category: Akiyoshi, B]]
[[Category: Marciano, G]]
[[Category: Ishii, M]]
[[Category: Nerusheva, O]]