OCA at 05:39, 5 July 2023

OCA — Wed, 05 Jul 2023 05:39:03 GMT

←Older revision		Revision as of 05:39, 5 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8a4g is ~~ON HOLD until Paper Publication~~	+	==IPNS H270E variant in complex with Cd and ACV==
-		+	<StructureSection load='8a4g' size='340' side='right'caption='[[8a4g]], [[Resolution\|resolution]] 1.97Å' scene=''>
-	~~Authors~~: ~~Rabe~~, P., ~~Schofield~~, C.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8a4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A4G FirstGlance]. <br>
-	~~Description~~: ~~IPNS H270E variant in complex with Cd and~~ ACV	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACV:L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE'>ACV</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: Rabe, P]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a4g OCA], [https://pdbe.org/8a4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a4g RCSB], [https://www.ebi.ac.uk/pdbsum/8a4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a4g ProSAT]</span></td></tr>
-	[[Category: Schofield~~, C.J~~]]	+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IPNA_EMENI IPNA_EMENI] Isopenicillin N synthase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic (PubMed:3319778, PubMed:11755401). IpnA catalyzes the cyclization of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) to form isopenicillin N (IPN) that contains the beta-lactam nucleus (PubMed:3319778, PubMed:11755401, PubMed:28703303). The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS acvA. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase penDE to yield penicillin in the peroxisomal matrix (By similarity).[UniProtKB:P08703]<ref>PMID:11755401</ref> <ref>PMID:28703303</ref> <ref>PMID:3319778</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aspergillus nidulans FGSC A4]]
		+	[[Category: Large Structures]]
		+	[[Category: Rabe P]]
		+	[[Category: Schofield CJ]]

OCA: Protected "8a4g" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2022 07:18:50 GMT

Protected "8a4g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:18, 22 June 2022

OCA: New page: '''Unreleased structure''' The entry 8a4g is ON HOLD until Paper Publication Authors: Rabe, P., Schofield, C.J. Description: IPNS H270E variant in complex with Cd and ACV [[Category: U...

OCA — Wed, 22 Jun 2022 07:18:49 GMT

New page: '''Unreleased structure''' The entry 8a4g is ON HOLD until Paper Publication Authors: Rabe, P., Schofield, C.J. Description: IPNS H270E variant in complex with Cd and ACV [[Category: U...

New page

'''Unreleased structure'''

The entry 8a4g is ON HOLD until Paper Publication

Authors: Rabe, P., Schofield, C.J.

Description: IPNS H270E variant in complex with Cd and ACV
[[Category: Unreleased Structures]]
[[Category: Rabe, P]]
[[Category: Schofield, C.J]]

8a4g - Revision history

OCA at 05:39, 5 July 2023

OCA: Protected "8a4g" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8a4g is ON HOLD until Paper Publication Authors: Rabe, P., Schofield, C.J. Description: IPNS H270E variant in complex with Cd and ACV [[Category: U...