OCA at 19:26, 19 October 2022

OCA — Wed, 19 Oct 2022 19:26:21 GMT

←Older revision		Revision as of 19:26, 19 October 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8abz is ~~ON HOLD~~	+	==RNA polymerase at U-rich pause bound to non-regulatory RNA - pause prone, closed clamp state==
		+	<StructureSection load='8abz' size='340' side='right'caption='[[8abz]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8abz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ABZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ABZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IGU:2-DEOXYISOGUANINE-5-MONOPHOSPHATE'>IGU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8abz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8abz OCA], [https://pdbe.org/8abz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8abz RCSB], [https://www.ebi.ac.uk/pdbsum/8abz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8abz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RPOA_ECOLI RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at intrinsic terminators in all kingdoms of life. In contrast, the regulatory RNA putL suppresses pausing and termination in cis. However, how nascent RNA modulates its own synthesis remains largely unknown. We present cryo-EM reconstructions of RNAP captured during transcription of putL variants or an unrelated sequence at a U-rich pause site. Our results suggest how putL suppresses pausing and promotes its synthesis. We demonstrate that transcribing a U-rich sequence, a ubiquitous trigger of intrinsic termination, promotes widening of the RNAP nucleic-acid-binding channel. Widening destabilizes RNAP interactions with DNA and RNA to facilitate transcript dissociation reminiscent of intrinsic transcription termination. Surprisingly, RNAP remains bound to DNA after transcript release. Our results provide the structural framework to understand RNA-mediated intrinsic transcription termination.

-	~~Authors: Dey~~, S., ~~Weixlbaumer~~, A.	+	Structural insights into RNA-mediated transcription regulation in bacteria.,Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A Mol Cell. 2022 Oct 7. pii: S1097-2765(22)00909-1. doi:, 10.1016/j.molcel.2022.09.020. PMID:36220101<ref>PMID:36220101</ref>

-	~~Description: RNA polymerase at~~ U-~~rich pause bound to non~~-~~regulatory RNA~~ -~~pause prone, closed clamp state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Dey, S]]	+	<div class="pdbe-citations 8abz" style="background-color:#fffaf0;"></div>
-	[[Category: Weixlbaumer, A]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Large Structures]]
		+	[[Category: Dey S]]
		+	[[Category: Weixlbaumer A]]

OCA: Protected "8abz" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2022 04:09:43 GMT

Protected "8abz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:09, 13 July 2022

OCA: New page: '''Unreleased structure''' The entry 8abz is ON HOLD Authors: Dey, S., Weixlbaumer, A. Description: RNA polymerase at U-rich pause bound to non-regulatory RNA -pause prone, closed clam...

OCA — Wed, 13 Jul 2022 04:09:42 GMT

New page: '''Unreleased structure''' The entry 8abz is ON HOLD Authors: Dey, S., Weixlbaumer, A. Description: RNA polymerase at U-rich pause bound to non-regulatory RNA -pause prone, closed clam...

New page

'''Unreleased structure'''

The entry 8abz is ON HOLD

Authors: Dey, S., Weixlbaumer, A.

Description: RNA polymerase at U-rich pause bound to non-regulatory RNA -pause prone, closed clamp state
[[Category: Unreleased Structures]]
[[Category: Dey, S]]
[[Category: Weixlbaumer, A]]

8abz - Revision history

OCA at 19:26, 19 October 2022

OCA: Protected "8abz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8abz is ON HOLD Authors: Dey, S., Weixlbaumer, A. Description: RNA polymerase at U-rich pause bound to non-regulatory RNA -pause prone, closed clam...