OCA at 07:30, 8 March 2023

OCA — Wed, 08 Mar 2023 07:30:20 GMT

←Older revision		Revision as of 07:30, 8 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8agu is ~~ON HOLD until Paper~~ Publication	+	==Yeast RQC complex in state E==
		+	<StructureSection load='8agu' size='340' side='right'caption='[[8agu]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8agu]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AGU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CT:HYPUSINE'>5CT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8agu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8agu OCA], [https://pdbe.org/8agu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8agu RCSB], [https://www.ebi.ac.uk/pdbsum/8agu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8agu ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RL35A_YEAST RL35A_YEAST]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. During RQC, dissociation of stalled ribosomes is followed by elongation of the nascent peptide with alanine and threonine residues, driven by Rqc2 independently of mRNA, the small ribosomal subunit and guanosine triphosphate (GTP)-hydrolyzing factors. The resulting CAT tails (carboxy-terminal tails) and ubiquitination by Ltn1 mark nascent peptides for proteasomal degradation. Here we present ten cryogenic electron microscopy (cryo-EM) structures, revealing the mechanistic basis of individual steps of the CAT tailing cycle covering initiation, decoding, peptidyl transfer, and tRNA translocation. We discovered eIF5A as a crucial eukaryotic RQC factor enabling peptidyl transfer. Moreover, we observed dynamic behavior of RQC factors and tRNAs allowing for processivity of the CAT tailing cycle without additional energy input. Together, these results elucidate key differences as well as common principles between CAT tailing and canonical translation.

-	~~Authors~~:	+	Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.,Tesina P, Ebine S, Buschauer R, Thoms M, Matsuo Y, Inada T, Beckmann R Mol Cell. 2023 Feb 16;83(4):607-621.e4. doi: 10.1016/j.molcel.2023.01.020. PMID:36804914<ref>PMID:36804914</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8agu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Beckmann R]]
		+	[[Category: Buschauer R]]
		+	[[Category: Tesina P]]

OCA at 16:36, 19 October 2022

OCA — Wed, 19 Oct 2022 16:36:07 GMT

←Older revision		Revision as of 16:36, 19 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8agu is ON HOLD	+	The entry 8agu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8agu" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2022 16:37:45 GMT

Protected "8agu" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:37, 27 July 2022

OCA: New page: '''Unreleased structure''' The entry 8agu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2022 16:37:44 GMT

New page: '''Unreleased structure''' The entry 8agu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8agu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8agu - Revision history

OCA at 07:30, 8 March 2023

OCA at 16:36, 19 October 2022

OCA: Protected "8agu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8agu is ON HOLD Authors: Description: Category: Unreleased Structures