8akh - Revision history

OCA at 08:12, 7 February 2024

OCA — Wed, 07 Feb 2024 08:12:28 GMT

←Older revision		Revision as of 08:12, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8akh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AKH FirstGlance]. <br>		<table><tr><td colspan='2'>[[8akh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AKH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8akh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8akh OCA], [https://pdbe.org/8akh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8akh RCSB], [https://www.ebi.ac.uk/pdbsum/8akh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8akh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8akh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8akh OCA], [https://pdbe.org/8akh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8akh RCSB], [https://www.ebi.ac.uk/pdbsum/8akh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8akh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0P7JVD2_LACPN A0A0P7JVD2_LACPN]		[https://www.uniprot.org/uniprot/A0A0P7JVD2_LACPN A0A0P7JVD2_LACPN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that lipoteichoic acids (LTAs) but not wall teichoic acids are D-alanylated in Lactiplantibacillus plantarum(NC8) cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum(NC8) and Drosophila melanogaster as a symbiosis model, we establish that D-alanylated LTAs (D-Ala-LTAs) are direct cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTAs to host physiology in other symbiotic models.
-
-	Structure-function analysis of Lactiplantibacillus plantarum DltE& reveals D-alanylated lipoteichoic acids as direct cues supporting Drosophila juvenile growth.,Nikolopoulos N, Matos R, Ravaud S, Courtin P, Akherraz H, Palussiere S, Gueguen-Chaignon V, Salomon-Mallet M, Guillot A, Guerardel Y, Chapot-Chartier MP, Grangeasse C, Leulier F Elife. 2023 Apr 12;12:e84669. doi: 10.7554/eLife.84669. PMID:37042660<ref>PMID:37042660</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8akh" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:03, 26 April 2023

OCA — Wed, 26 Apr 2023 17:03:35 GMT

←Older revision		Revision as of 17:03, 26 April 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8akh is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of DltE from L. plantarum soaked with LTA==
		+	<StructureSection load='8akh' size='340' side='right'caption='[[8akh]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8akh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AKH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8akh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8akh OCA], [https://pdbe.org/8akh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8akh RCSB], [https://www.ebi.ac.uk/pdbsum/8akh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8akh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0P7JVD2_LACPN A0A0P7JVD2_LACPN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that lipoteichoic acids (LTAs) but not wall teichoic acids are D-alanylated in Lactiplantibacillus plantarum(NC8) cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum(NC8) and Drosophila melanogaster as a symbiosis model, we establish that D-alanylated LTAs (D-Ala-LTAs) are direct cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTAs to host physiology in other symbiotic models.

-	~~Authors~~:	+	Structure-function analysis of Lactiplantibacillus plantarum DltE& reveals D-alanylated lipoteichoic acids as direct cues supporting Drosophila juvenile growth.,Nikolopoulos N, Matos R, Ravaud S, Courtin P, Akherraz H, Palussiere S, Gueguen-Chaignon V, Salomon-Mallet M, Guillot A, Guerardel Y, Chapot-Chartier MP, Grangeasse C, Leulier F Elife. 2023 Apr 12;12:e84669. doi: 10.7554/eLife.84669. PMID:37042660<ref>PMID:37042660</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8akh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lactiplantibacillus plantarum]]
		+	[[Category: Large Structures]]
		+	[[Category: Grangeasse C]]
		+	[[Category: Nikolopoulos N]]
		+	[[Category: Ravaud S]]

OCA at 15:22, 7 September 2022

OCA — Wed, 07 Sep 2022 15:22:32 GMT

←Older revision		Revision as of 15:22, 7 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8akh is ON HOLD	+	The entry 8akh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8akh" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2022 03:54:53 GMT

Protected "8akh" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:54, 10 August 2022

OCA: New page: '''Unreleased structure''' The entry 8akh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Aug 2022 03:54:52 GMT

New page: '''Unreleased structure''' The entry 8akh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8akh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]